Delivering siRNA to control osteogenic differentiation and real-time detection of cell differentiation in human mesenchymal stem cells using multifunctional gold nanoparticles

Wu, Qian; Wang, Kaipeng; Wang, Xichao; Liang, Guohai; Li, Jinming

doi:10.1039/c9tb02899d

Cited by 18 publications

(13 citation statements)

References 38 publications

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“…In the field of bone and cartilage TE, it has been reported that AuNPs can deliver siRNA, miRNA and other biomacromolecules with the assistance of various modification (Pan et al, 2016;Wu et al, 2020). AuNPs as effective biomacromolecules delivery carriers provide adequate protection to prevent them from being degraded by enzymes.…”

Section: Biomacromolecules Deliverymentioning

confidence: 99%

Gold Nanomaterials and Bone/Cartilage Tissue Engineering: Biomedical Applications and Molecular Mechanisms

et al. 2021

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Recently, as our population increasingly ages with more pressure on bone and cartilage diseases, bone/cartilage tissue engineering (TE) have emerged as a potential alternative therapeutic technique accompanied by the rapid development of materials science and engineering. The key part to fulfill the goal of reconstructing impaired or damaged tissues lies in the rational design and synthesis of therapeutic agents in TE. Gold nanomaterials, especially gold nanoparticles (AuNPs), have shown the fascinating feasibility to treat a wide variety of diseases due to their excellent characteristics such as easy synthesis, controllable size, specific surface plasmon resonance and superior biocompatibility. Therefore, the comprehensive applications of gold nanomaterials in bone and cartilage TE have attracted enormous attention. This review will focus on the biomedical applications and molecular mechanism of gold nanomaterials in bone and cartilage TE. In addition, the types and cellular uptake process of gold nanomaterials are highlighted. Finally, the current challenges and future directions are indicated.

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Section: Biomacromolecules Deliverymentioning

confidence: 99%

Gold Nanomaterials and Bone/Cartilage Tissue Engineering: Biomedical Applications and Molecular Mechanisms

et al. 2021

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“…Gold nanoparticles (AuNPs) have been widely studied in medical fields, such as imaging, drug delivery, and theragnostic systems [ 152 ]. Wu et al [ 153 ] developed multifunctional AuNPs to control cell fate and simultaneously detect the osteogenic differentiation of hMSCs in real time. AuNP-polyethyleneimine-peptide-fluorescein isothiocyanate/small molecule-interfering RNA (AuNP-PEI-peptide-FITC/siRNA) nanocomplexes to control the osteogenic differentiation of hMSCs could be silenced by the peroxisome proliferator-activated receptor γ (PPARγ), an adipogenesis-related gene.…”

Section: Latest Technological Advances In Monitoring Stem Cell Status...mentioning

confidence: 99%

Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

et al. 2022

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In regenerative medicine, cell therapies using various stem cells have received attention as an alternative to overcome the limitations of existing therapeutic methods. Clinical applications of stem cells require the identification of characteristics at the single-cell level and continuous monitoring during expansion and differentiation. In this review, we recapitulate the application of various stem cells used in regenerative medicine and the latest technological advances in monitoring the differentiation process of stem cells. Single-cell RNA sequencing capable of profiling the expression of many genes at the single-cell level provides a new opportunity to analyze stem cell heterogeneity and to specify molecular markers related to the branching of differentiation lineages. However, this method is destructive and distorted. In addition, the differentiation process of a particular cell cannot be continuously tracked. Therefore, several spectroscopic methods have been developed to overcome these limitations. In particular, the application of Raman spectroscopy to measure the intrinsic vibration spectrum of molecules has been proposed as a powerful method that enables continuous monitoring of biochemical changes in the process of the differentiation of stem cells. This review provides a comprehensive overview of current analytical methods employed for stem cell engineering and future perspectives of nano-biosensing technologies as a platform for the in situ monitoring of stem cell status and differentiation.

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“…Gold nanoparticles (AuNPs) are one of the most popular nanomaterials for biosensing applications due to their unique optical and electrical properties, large surface area, easy surface modification, excellent biocompatibility, high extinction coefficients, and potential noncytotoxicity. [25][26][27][28] Herein, we develop a new gold nanoparticle-based single-molecule biosensor for simple and sensitive detection of Ago2 activity. In this biosensor, multiple Cy5-labeled signal probes are assembled on AuNPs to construct a fluorescence-quenched AuNP nanoprobe.…”

Section: Introductionmentioning

confidence: 99%

Construction of a gold nanoparticle-based single-molecule biosensor for simple and sensitive detection of Argonaute 2 activity

Jiang

Zhang

et al. 2022

J. Mater. Chem. B

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Delivering siRNA to control osteogenic differentiation and real-time detection of cell differentiation in human mesenchymal stem cells using multifunctional gold nanoparticles

Abstract:
Schematic representation of the multifunctional gold nanoparticles (AuNP-PEI-peptide-FITC) synthesis and siRNA adsorption to silence the PPARγ gene for controlling osteogenic differentiation and real-time detection of ongoing cell differentiation in hMSCs.

Cited by 18 publications

References 38 publications

Gold Nanomaterials and Bone/Cartilage Tissue Engineering: Biomedical Applications and Molecular Mechanisms

Gold Nanomaterials and Bone/Cartilage Tissue Engineering: Biomedical Applications and Molecular Mechanisms

Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

Construction of a gold nanoparticle-based single-molecule biosensor for simple and sensitive detection of Argonaute 2 activity

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Delivering siRNA to control osteogenic differentiation and real-time detection of cell differentiation in human mesenchymal stem cells using multifunctional gold nanoparticles

Abstract: Schematic representation of the multifunctional gold nanoparticles (AuNP-PEI-peptide-FITC) synthesis and siRNA adsorption to silence the PPARγ gene for controlling osteogenic differentiation and real-time detection of ongoing cell differentiation in hMSCs.

Cited by 18 publications

References 38 publications

Gold Nanomaterials and Bone/Cartilage Tissue Engineering: Biomedical Applications and Molecular Mechanisms

Gold Nanomaterials and Bone/Cartilage Tissue Engineering: Biomedical Applications and Molecular Mechanisms

Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

Construction of a gold nanoparticle-based single-molecule biosensor for simple and sensitive detection of Argonaute 2 activity

Contact Info

Product

Resources

About

Abstract:
Schematic representation of the multifunctional gold nanoparticles (AuNP-PEI-peptide-FITC) synthesis and siRNA adsorption to silence the PPARγ gene for controlling osteogenic differentiation and real-time detection of ongoing cell differentiation in hMSCs.