Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

Kim, Wijin; Park, Eungyeong; Yoo, Hyuk Sang; Park, Jong-Min; Jung, Young Mee; Park, Ju Hyun

doi:10.3390/nano12172934

Cited by 5 publications

(5 citation statements)

References 212 publications

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“…Additionally, in larger spheroids, stem cell markers may not effectively penetrate the core of the spheroid, limiting the ability to assess the stem cell population in the inner regions [ 176 , 177 ]. Researchers can employ several strategies to overcome these challenges and effectively use stem cell markers in 3D spheroid cultures [ 178 , 179 ]. An alternative method involves combining stem cells and other cellular markers to better understand the cellular composition within the spheroid.…”

Section: Challenges and Future Prospectivesmentioning

confidence: 99%

“…Controlling the size of spheroids is another strategy to enhance marker penetration and access to the innermost cells. Utilizing microfluidic techniques allows for the accurate regulation of spheroid size, ensuring effective penetration of markers throughout all regions of the spheroid [ 178 , 179 ]. Additionally, single-cell analysis methods, such as single-cell RNA sequencing and proteomic analysis, enable the characterization of individual cells within spheroids.…”

Section: Challenges and Future Prospectivesmentioning

confidence: 99%

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Scaffold-based 3D cell culture models in cancer research

Abuwatfa,

Pitt,

Husseini

2024

J Biomed Sci

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Three-dimensional (3D) cell cultures have emerged as valuable tools in cancer research, offering significant advantages over traditional two-dimensional (2D) cell culture systems. In 3D cell cultures, cancer cells are grown in an environment that more closely mimics the 3D architecture and complexity of in vivo tumors. This approach has revolutionized cancer research by providing a more accurate representation of the tumor microenvironment (TME) and enabling the study of tumor behavior and response to therapies in a more physiologically relevant context. One of the key benefits of 3D cell culture in cancer research is the ability to recapitulate the complex interactions between cancer cells and their surrounding stroma. Tumors consist not only of cancer cells but also various other cell types, including stromal cells, immune cells, and blood vessels. These models bridge traditional 2D cell cultures and animal models, offering a cost-effective, scalable, and ethical alternative for preclinical research. As the field advances, 3D cell cultures are poised to play a pivotal role in understanding cancer biology and accelerating the development of effective anticancer therapies. This review article highlights the key advantages of 3D cell cultures, progress in the most common scaffold-based culturing techniques, pertinent literature on their applications in cancer research, and the ongoing challenges. Graphical Abstract

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Section: Challenges and Future Prospectivesmentioning

confidence: 99%

Section: Challenges and Future Prospectivesmentioning

confidence: 99%

Scaffold-based 3D cell culture models in cancer research

Abuwatfa,

Pitt,

Husseini

2024

J Biomed Sci

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“…Super-resolution imaging and single molecular tools for in-vitro nano-manipulation have made it easier to identify and characterize the molecules and the mechanics of structural transformations within mesenchymal stem cells and matrices. These advancements facilitate the exploration of the mesenchymal stem cell niche and aid in the development of new classes of mesenchymal stem cell-based nanoformulations that facilitate the production of “used biomaterials” for applications in tissue engineering and regenerative medicine [ 293 , 294 ].…”

Section: Pharmacological and Immunological Barriers In Stem Cell Memb...mentioning

confidence: 99%

Precision Nanomedicine with Bio-Inspired Nanosystems: Recent Trends and Challenges in Mesenchymal Stem Cells Membrane-Coated Bioengineered Nanocarriers in Targeted Nanotherapeutics

Baig,

Ahmad,

Pathan

et al. 2024

JoX

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In the recent past, the formulation and development of nanocarriers has been elaborated into the broader fields and opened various avenues in their preclinical and clinical applications. In particular, the cellular membrane-based nanoformulations have been formulated to surpass and surmount the limitations and restrictions associated with naïve or free forms of therapeutic compounds and circumvent various physicochemical and immunological barriers including but not limited to systemic barriers, microenvironmental roadblocks, and other cellular or subcellular hinderances—which are quite heterogeneous throughout the diseases and patient cohorts. These limitations in drug delivery have been overcome through mesenchymal cells membrane-based precision therapeutics, where these interventions have led to the significant enhancements in therapeutic efficacies. However, the formulation and development of nanocarriers still focuses on optimization of drug delivery paradigms with a one-size-fits-all resolutions. As mesenchymal stem cell membrane-based nanocarriers have been engineered in highly diversified fashions, these are being optimized for delivering the drug payloads in more and better personalized modes, entering the arena of precision as well as personalized nanomedicine. In this Review, we have included some of the advanced nanocarriers which have been designed and been utilized in both the non-personalized as well as precision applicability which can be employed for the improvements in precision nanotherapeutics. In the present report, authors have focused on various other aspects of the advancements in stem cells membrane-based nanoparticle conceptions which can surmount several roadblocks and barriers in drug delivery and nanomedicine. It has been suggested that well-informed designing of these nanocarriers will lead to appreciable improvements in the therapeutic efficacy in therapeutic payload delivery applications. These approaches will also enable the tailored and customized designs of MSC-based nanocarriers for personalized therapeutic applications, and finally amending the patient outcomes.

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“…Addressing these two fundamental questions will enable researchers to better define their requirements for in vitro tumorigenicity assessment. In addition to the manual efforts, the proper use of advanced technologies, such as automated robotics [107,108], sensitive biosensors [109,110], and machine intelligence [111][112][113], may also help the academia and industry to reach a global consensus on tumorigenicity assessment.…”

Section: Outlook and Conclusionmentioning

confidence: 99%

Assessing Tumorigenicity in Stem Cell-Derived Therapeutic Products: A Critical Step in Safeguarding Regenerative Medicine

Wang

2023

Bioengineering

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Stem cells hold promise in regenerative medicine due to their ability to proliferate and differentiate into various cell types. However, their self-renewal and multipotency also raise concerns about their tumorigenicity during and post-therapy. Indeed, multiple studies have reported the presence of stem cell-derived tumors in animal models and clinical administrations. Therefore, the assessment of tumorigenicity is crucial in evaluating the safety of stem cell-derived therapeutic products. Ideally, the assessment needs to be performed rapidly, sensitively, cost-effectively, and scalable. This article reviews various approaches for assessing tumorigenicity, including animal models, soft agar culture, PCR, flow cytometry, and microfluidics. Each method has its advantages and limitations. The selection of the assay depends on the specific needs of the study and the stage of development of the stem cell-derived therapeutic product. Combining multiple assays may provide a more comprehensive evaluation of tumorigenicity. Future developments should focus on the optimization and standardization of microfluidics-based methods, as well as the integration of multiple assays into a single platform for efficient and comprehensive evaluation of tumorigenicity.

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Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

Cited by 5 publications

References 212 publications

Scaffold-based 3D cell culture models in cancer research

Scaffold-based 3D cell culture models in cancer research

Precision Nanomedicine with Bio-Inspired Nanosystems: Recent Trends and Challenges in Mesenchymal Stem Cells Membrane-Coated Bioengineered Nanocarriers in Targeted Nanotherapeutics

Assessing Tumorigenicity in Stem Cell-Derived Therapeutic Products: A Critical Step in Safeguarding Regenerative Medicine

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