Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L.

Abstract

Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L. A pale-pink strain (CC-FR2-10 T ) from the rhizosphere of the sacred tree Ficus religiosa L. in Taiwan was investigated by using a polyphasic taxonomic approach. The cells were Gram-positive, rod-shaped and non-spore-forming. Phylogenetic analyses using the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus, the highest sequence similarities being found with Deinococcus grandis (96?1 %), Deinococcus radiodurans (94?3 %), Deinococcus radiopugnans (93?2 %), Deinococcus indicus (93?0 %), Deinococcus proteolyticus (92?5 %), Deinococcus murrayi (92?4 %) and Deinococcus geothermalis (90?7 %). The DNA-DNA relatedness with respect to D. grandis DSM 3963 T was 17?9 %. Chemotaxonomic data revealed that strain CC-FR2-10 T contains only menaquinone MK-8 as the respiratory quinone, unknown phosphoglycolipids as the predominant polar lipids and 16 : 1v7c, 17 : 1v8c and 17 : 1v9c iso as the predominant fatty acids. The biochemical and chemotaxonomic properties demonstrate that strain CC-FR2-10 T represents a novel species, for which the name Deinococcus ficus sp. nov. is proposed. The type strain is

Deinococcus depolymerans sp. nov., a gamma- and UV-radiation-resistant bacterium, isolated from a naturally radioactive site

“…S4). These results are in agreement with previous results reported for species of the genus Deinococcus (Ferreira et al, 1997;de Groot et al, 2005;Lai et al, 2006;Kämpfer et al, 2008); however, strain TDMA-24 T showed a unique combination of unknown polar lipids, which might distinguish them from other members of the genus Deinococcus. For instance, strain TDMA-24 T contained unidentified phosphoglycolipid PGL3, seven unidentified lipids and five unidentified glycolipids, whereas D. aquaticus contained no PGL3, only four unidentified lipids and six unidentified glycolipids (Im et al, 2008).…”

“…The four strains contained a quinone system composed predominantly of MK-8. This is in agreement with the menaquinone systems of other species of the genus Deinococcus, which all contain menaquinone MK-8 as the major quinone (Embley et al, 1987;Ferreira et al, 1997;Kämpfer et al, 2008;Lai et al, 2006;Suresh et al, 2004).…”

“…The genus Deinococcus (Battista & Rainey, 2001;Brooks & Murray, 1981;Rainey et al, 1997) belongs to the family Deinococcaceae (Battista & Rainey, 2001;Brooks & Murray, 1981;Rainey et al, 1997) and comprises .40 species, 26 of which have been described as being highly resistant to radiation (Asker et al, 2008(Asker et al, , 2009de Groot et al, 2005;Hirsch et al, 2004;Im et al, 2008;Lai et al, 2006;Rainey et al, 2005;Suresh et al, 2004;Zhang et al, 2007). Four other species of the genus Deinococcus have been described as being sensitive to ionizing radiation (Callegan et al, 2008).…”

Deinococcus aquatilis sp. nov., isolated from water

“…Results are given in the species description. Strain CCUG 53370 T was unable to utilize L-arabinose, lactose, trehalose, D-xylose, D-mannose, melibiose or D-sorbitol and utilized N-acetyl-D-glucosamine weakly; in contrast, D. ficus CC-FR2-10 T utilized all of these compounds (Lai et al, 2006).…”

“…The taxonomy of the genus has been described extensively (Suresh et al, 2004;Hirsch et al, 2004;Ferreira et al, 1997;Rainey et al, 1997Rainey et al, , 2005, and the number of species is still growing (Asker et al, 2009;Callegan et al, 2008;de Groot et al, 2005;Lai et al, 2006;Rainey et al, 2007;Shashidhar & Bandekar, 2006;Zhang et al, 2007). Several novel Deinococcus strains have been isolated and well characterized from soils, including desert soil, foods, faeces and dust, with additional data on their extreme resistance to UV and gamma radiation and desiccation.…”