Degradative pathways in cultured synovial fibroblasts: Selective effects of pulsed electromagnetic fields

Abstract

A cell culture model for studying the cytokine-mediated degradation of connective tissue was exposed to clinically applied, low-frequency pulsed electromagnetic fields (PEMF), and levels of collagenolytic activity, two lysosomal hydrolases, and prostaglandin E2 were measured. PEMFs reduced the release of two lysosomal enzymes by cultured rabbit synovial fibroblasts but did not affect their response to mononuclear-cell-conditioned medium. PEMF did not alter levels of cytokine activity produced by a mixed mononuclear cell population, nor did they affect the cytokine-mediated release of collagenase or prostaglandin E2 by synovial fibroblasts. The relevance of these findings to the clinical application of PEMF to soft- and hard-tissue injuries is discussed.

Electromagnetic fields (EMFs) and adenosine receptors modulate prostaglandin E2 and cytokine release in human osteoarthritic synovial fibroblasts

“…To our knowledge, very few studies have investigated the EMF effect on SFs and no data have been reported on human OASFs and the modulation of cytokine release (Murray et al, 1988; Marino et al, 2003; Varani et al, 2008; De Mattei et al, 2009). On the other hand, our data agree with previous studies showing that EMFs inhibit PGE 2 and cytokine production both in “in vitro” and in “in vivo” models, as well as in clinical studies (Lohmann et al, 2000; Chang et al, 2005; Benazzo et al, 2008a; De Mattei et al, 2009; Gomez‐Ochoa et al, 2010; Rohde et al, 2010).…”

Pulsed Electromagnetic Field Inhibits Synovitis via Enhancing the Efferocytosis of Macrophages

“…Previous studies reported that PEMF could inhibit the secretion of inflammatory factors including IL-1β and TNF-α in several types of cells [23][24][25], indicating that the PEMF has a certain anti-inflammatory effect. Murray et al found that PEMF can reduce the release of lysosomal enzymes from rabbit synovial fibroblasts without affecting the release of collagenase and prostaglandin E2 [26]. De Mattei et al reported that PEMF can reduce the release of prostaglandin E2 from bovine synovial fibroblasts, and this inhibition is achieved by upregulating adenosine receptor [27].…”

Einfluß statischer magnetischer Felder auf die Prostaglandinsynthese in L-929- und 3T3-Maus-Fibroblasten

“…W~ihrend die Effekte von starken elektromagnetischen Wechselfeldern sehr gut untersucht wurden [19,21,26,[28][29][30]32], liegen fiber die Auswirkung statisch magnetischer Felder bis heute nur vereinzelt Untersuchungen vor [13,32]. W~ihrend die Effekte von starken elektromagnetischen Wechselfeldern sehr gut untersucht wurden [19,21,26,[28][29][30]32], liegen fiber die Auswirkung statisch magnetischer Felder bis heute nur vereinzelt Untersuchungen vor [13,32].…”