Einfluß statischer magnetischer Felder auf die Prostaglandinsynthese in L-929- und 3T3-Maus-Fibroblasten

Abstract

The aim of this study was to examine the influence of static, inhomogeneous magnetic fields on prostaglandin synthesis in L-929 and 3T3 mouse fibroblasts. Two pairs of magnets, one CoSm and the other NdFeB, were attached 10 mm apart under the culture chamber. One pair was in the attracting position and the other in the repelling position. The maximum magnetic induction measured was 85 respectively 120 mT and the incubation time was 72 hours. Control groups consisted of untreated cells and cells under the influence of demagnetized magnets. The following measurements were taken: prostaglandin synthesis (PGE2), protein content (GP), and the number (Z) of cells (only L-929 mouse fibroblasts). The data were analyzed statistically using the Tukey test and the significance niveau was set at alpha = 0.05. The results using this method demonstrated that the measurable effects of in vitro treatment of mouse fibroblasts with static magnetic fields of varying induction did not significantly differ from the control groups. The sensitivity of our method was verified beforehand with a recombinant, human TNF alpha stimulated reference group.

Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability

“…It has been previously proposed that deadenylation is the rate limiting step of mRNA decay ( Breunig et al, 1993 ). The observation that mRNA half-lives positively correlate when measured using polyA selection compared to measurements in the absence of polyA enrichment support this model ( Figure 1—figure supplement 2B ).…”