Degradation pattern of porous CaCO 3 and hydroxyapatite microspheres in vitro and in vivo for potential application in bone tissue engineering

Zhong, Qiwei; Li, Wenhua; Su, Xiuping; Luo, Geng; Zhou, Ying; Kundu, Subhas C.; Yao, Juming; Cai, Yurong

doi:10.1016/j.colsurfb.2016.03.020

Cited by 50 publications

(31 citation statements)

References 35 publications

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“…HE et al, showed that CaCO 3 sintered at a low temperature (650 °C) is not robust enough and high degradation might be seen 21) . Moreover, compounds with higher contents of CaCO 3 are degraded at a higher rate 33,34) . Hence, we consider that CaTiO 3 -CaCO 3 heat treated for 30 min and therefore with higher CaCO 3 content might induce rapid release of Ca 2+ to the environment resulting in larger areas of mineralized matrix around the cells.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Efficacy of CaCO3 Content in CaTiO3– CaCO3 Composites for Bone Growth

Rodrı́guez

Sánchez

Felice

et al. 2018

J. Hard Tissue Biology.

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The eff ect of CaTiO 3 compounded with diff erent amounts of CaCO 3 on osteoblastic KUSA/A1 cells was evaluated. CaTiO 3 -CaCO 3 composites were obtained by alkoxide method, a simple, low-cost and reproducible technique used for largescale production of material. The content of CaCO 3 in our samples was controlled by varying the sintering time of the overall process. Composite morphology was assessed by scanning electron microscopy (SEM) showing particles with sizes ranging from100 to 500 nm. The presence of CaCO 3 was revealed by XRD and thermogravimetric analyses, which suggested that samples treated at 650ºC for 30 min contained higher amounts of CaCO 3 than samples treated for 2 and 10 h. Additionally, in vitro studies demonstrated that CaTiO 3 -CaCO 3 composites sintered for 30 min induced augmented cell proliferation and mineralization in comparison to composites sintered for longer periods of time. Hence, our fi ndings clearly suggest that the amount of CaCO 3 within CaTiO 3 -CaCO 3 composites exerts a critical eff ect on osteoblastic cells response. Enhanced bone regeneration could be achieved by increasing the content of CaCO 3 within the composites, thus establishing CaTiO 3 -CaCO 3 as a promising material for bone augmentation procedures in dental fi eld.

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Section: Discussionmentioning

confidence: 99%

In Vitro Efficacy of CaCO3 Content in CaTiO3– CaCO3 Composites for Bone Growth

Rodrı́guez

Sánchez

Felice

et al. 2018

J. Hard Tissue Biology.

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“…Alginic acid sodium salt from brown algae (Sigma-Aldrich, St. Louis, MO, USA) and HA were purchased for this study. The preparation procedures for AL, AL/HA, and AL/HA/SF were described in previous publications [62,63]. Briefly, Mulberry silk cocoons were cut and degummed in 0.2 M Na 2 CO 3 for 30 min followed by drying at 37 °C for 24 h. Degummed fibers were dissolved in 9.3 M LiBr and incubated for 4 h at 60 °C.…”

Section: Methodsmentioning

confidence: 99%

Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo

Kim

Kwon

et al. 2017

IJMS

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The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites. Forty Sprague Dawley rats were used for the animal experiments. Central calvarial bone (diameter: 8.0 mm) defects were grafted with AL, AL/HA, or AL/HA/SF. New bone formation was evaluated by histomorphometric analysis. To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation. Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation. The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation. Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation. IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation. Additionally, no evidence of the inflammatory reaction or giant cell formation was observed around the residual graft material. We concluded that the AL/HA/SF composite could be effective as a scaffold for bone tissue engineering.

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“…A series of experiments on temperature dependence of as-prepared calcium carbonate were carried out to obtain thermodynamic adsorption parameters, such as standard Gibbs free energy (∆G θ , J•mol −1 ), enthalpy (∆H θ , J•mol −1 ), and entropy (∆S θ , J•mol −1 •K −1 ) [24], which can be determined using Equations (6) and 7:…”

Section: Thermodynamic Parameters Analysismentioning

confidence: 99%

“…An imposing specific surface area, good biocompatibility and non-toxic are the obvious features of porous calcium carbonate. Therefore, porous calcium carbonate is widely applied into all walks of life, such as drug carriers [2][3][4], bioceramics [5,6], biomicrocapsules [7,8], biosensors [9], bone repair [10], biomimetic mineralization [6,11], and so on. Generally, material structures and crystal forms, affected strongly by preparation and operation conditions, have rather significant effects on their application.…”

Section: Introductionmentioning

confidence: 99%

The Characterization and Amoxicillin Adsorption Activity of Mesopore CaCO3 Microparticles Prepared Using Rape Flower Pollen

et al. 2019

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A precipitation reaction method was employed to prepare mesopore calcium carbonate (CaCO3) using rape flower pollen as the template. CaCO3 adsorbent was characterized using X-ray diffraction (XRD), scanning electronic microscopy (SEM), and Brunner−Emmet−Teller measurements (BET). The equilibrium adsorption data on amoxicillin were explained using Langmuir, Freundlich, and Temkin adsorption isotherm models. The pseudo-first order, second order, pseudo-second order, and intra-particle diffusion kinetic models were used to explore adsorption kinetics. Equilibrium adsorption of as-prepared CaCO3 was better depicted using the Langmuir adsorption model with an R2 of 0.9948. The separation factor (RL) was found to be in the range of 0 < RL < 1, indicating the favorable adsorption of amoxicillin. The adsorption capacity of mesopore CaCO3 reached 13.49 mg·g−1 in 0.2 g∙L−1 amoxicillin solution. The values of adsorption thermodynamic parameters (ΔHθ, ΔSθ, ΔGθ) were obtained. In addition, the adsorption process turned out to be endothermic and spontaneous for the CaCO3 product at 298 K, 308 K, and 318 K.

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Degradation pattern of porous CaCO 3 and hydroxyapatite microspheres in vitro and in vivo for potential application in bone tissue engineering

Cited by 50 publications

References 35 publications

<i>In Vitro</i> Efficacy of CaCO<sub>3</sub> Content in CaTiO<sub>3</sub>– CaCO<sub>3</sub> Composites for Bone Growth

<i>In Vitro</i> Efficacy of CaCO<sub>3</sub> Content in CaTiO<sub>3</sub>– CaCO<sub>3</sub> Composites for Bone Growth

Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo

The Characterization and Amoxicillin Adsorption Activity of Mesopore CaCO3 Microparticles Prepared Using Rape Flower Pollen

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