Degradation of tetracycline antibiotics by Arthrobacter nicotianae OTC-16

Shi, Yanke; Lin, Hui; Ma, Junwei; Zhu, Rongrong; Sun, Wanchun; Lin, Xiaoyan; Zhang, Jin; Zheng, Huabao; Zhang, Xin

doi:10.1016/j.jhazmat.2020.123996

Cited by 87 publications

(30 citation statements)

References 53 publications

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“…Overall, OTC evidently up-regulated the expression of these ARGs transiently, and the short-term up-regulation effect might play an important role in the biodegradation of OTC by strain OTC-16. Our previous studies confirmed that the presence of OTC inhibited early growth and reduced the biodegradation rate of strain OTC-16 at the initial stage 12 , but biodegradation improved rapidly 48 h later with a high expression of ARGs. The up-regulation of genes sul1 and ant2ia by OTC (Fig.…”

Section: Resultssupporting

confidence: 81%

“…The strain shows interesting biodegradation of oxytetracycline (OTC)/tetracycline in various matrices, such as in aqueous media under a wide range of pH, temperature, and antibiotic concentration; manure samples from different animals; and soils with different properties 12 . Preliminary analysis 12 showed that the application of strain OTC-16 did not increase some commonly studied tetracycline-resistant genes in the soil, such as tetW , tetB , tetA, and tetG , and could effectively inhibit the proliferation of tetW and tetB . Nonetheless, these results are insufficient to conclude that there is no risk in applying this degrading bacterium in the environment.…”

Section: Introductionmentioning

confidence: 99%

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Genomic insights into the antibiotic resistance pattern of the tetracycline-degrading bacterium, Arthrobacter nicotianae OTC-16

Zhang

Zhu

et al. 2021

Sci Rep

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Although many bacteria have the potential to remove antibiotic residues from environmental niches, the benefits of using antibiotic-degrading bacteria to manage antibiotic pollution should be assessed against the risk of the potential expansion of antimicrobial resistance. This study investigated the antibiotic resistance pattern of the bacterium Arthrobacter nicotianae OTC-16, which shows substantial biodegradation of oxytetracycline (OTC)/tetracycline. The results showed that this strain could be resistant to at least seven categories of 15 antibiotics, based on antimicrobial susceptibility testing. The genome of A. nicotianae OTC-16 contains one chromosome (3,643,989 bp) and two plasmids (plasmid1, 123,894 bp and plasmid2, 29,841 bp). Of the 3,561 genes isolated, eight were related to antibiotic resistance. During OTC degradation by the strain OTC-16, the expression of ant2ia, sul1, tet33, and cml_e8 in the plasmid, and one gene (tetV) in the chromosome were tracked using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Only the plasmid-derived resistance genes were up-regulated in the presence of OTC. The presence of OTC increased the tolerance of strain OTC-16 to streptomycin sulphate. The findings of this study can help deepen our understanding of the behavioural characteristics of resistance genes and adaptive evolution of drug-resistant bacteria.

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Section: Resultssupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Genomic insights into the antibiotic resistance pattern of the tetracycline-degrading bacterium, Arthrobacter nicotianae OTC-16

Zhang

Zhu

et al. 2021

Sci Rep

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“…The results of optimization experiments showed that strain M503 expressed a high degradation rate at a temperature range of 20 °C–40 °C. The highest degradation rate occurred at 35 °C, which was a higher temperature than some bacterial strains, such as S. maltophilia DT1, A. nicotianae OTC-16, B. naejangsanensis DD1, and S. mizutaii DD2 [ 34 , 35 , 49 ]. The difference in optimal temperature between bacteria and strain M503 showed that strain M503 might prefer a higher temperature to perform degradation functions.…”

Section: Discussionmentioning

confidence: 99%

“…This is evidence that the antibacterial potency of TC degradation products by strain M503 decreased. Similarly, many studies found that the biotransformation products of TCs have a lower antibacterial potency [ 35 , 49 ]. This finding is an indication that microorganisms have the intellective mechanism to decrease the toxicity of antibiotics to bacteria.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of Tetracycline Antibiotics by the Yeast Strain Cutaneotrichosporon dermatis M503

Tan

Kong

et al. 2022

Microorganisms

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In this study, the Cutaneotrichosporon dermatis strain M503 was isolated and could efficiently degrade tetracycline, doxycycline, and chlorotetracyline. The characteristics of tetracycline degradation were investigated under a broad range of cultural conditions. Response surface methodology (RSM) predicted that the highest degradation rate of tetracycline could be obtained under the following conditions: 39.69 °C, pH of 8.79, and inoculum dose of 4.0% (v/v, ~3.5 × 106 cells/mL in the medium). In accordance with the five identified degradation products of tetracycline, two putative degradation pathways, which included the shedding of methyl and amino groups, were proposed. Moreover, the well diffusion method showed that the strain of M503 decreases the antibacterial potency of tetracycline, doxycycline, and chlorotetracycline. These findings proposed a putative mechanism of tetracycline degradation by a fungus strain and contributed to the estimation of the fate of tetracycline in the aquatic environment.

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“…We previously isolated a tetracycline antibiotic-degrading bacterium Arthrobacter nicotianae OTC-16, which shows striking biodegradation of oxytetracycline/tetracycline in various matrices, for example, aqueous media under a wide range of pH, temperature, and antibiotic concentration; manure samples from different animals; and soils with different properties 10 . Preliminary analysis showed that the application of strain OTC-16 did not increase some commonly studied tetracycline-resistant genes in the soil such as tetW, tetB, tetA, and tetG, and could effectively inhibit the proliferation of tetW and tetB.…”

Section: Introductionmentioning

confidence: 99%

Genomic Insights Into Antibiotic Resistance Pattern of Tetracycline Degrading Bacterium Arthrobacter Nicotianae OTC-16

Zhang

Zhu

et al. 2021

Preprint

Self Cite

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Although many bacteria have the potential to remove antibiotic residues from environmental niches, the benefits of using antibiotic degrading bacteria to manage antibiotic pollution should be assessed against the risk of the potential expansion of antimicrobial resistance. This study investigated the antibiotic resistance pattern of the bacterium Arthrobacter nicotianae OTC 16, which shows substantial biodegradation of oxytetracycline/tetracycline. The results showed that this strain could be resistant to at least seven categories, 15 antibiotics, based on antimicrobial susceptibility testing. The genome of A. nicotianae OTC 16 contains one chromosome(3,643,989 bp) and two plasmids (plasmid1,123,894 bp and plasmid2, 29,841bp). Of the 3,561 genes isolated, eight were related to antibiotic resistance. During oxytetracycline (OTC) degradation by the strain OTC 16, the expression of ant2ia, sul1, tet33, and cml_e8 in the plasmid, and one gene(tetV) in the chromosome were tracked by real time quantitative reverse transcription polymerase chain reaction(qRT PCR). Only the plasmid derived resistance genes were up regulated under the pressure of OTC. The presence of OTC increased the tolerance of strain OTC 16 to streptomycin sulphate. This work deepens our understanding of the behavioural characteristics of resistance genes and adaptive evolution of resistant drug bacteria under the pressure of antibiotics.

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Degradation of tetracycline antibiotics by Arthrobacter nicotianae OTC-16

Cited by 87 publications

References 53 publications

Genomic insights into the antibiotic resistance pattern of the tetracycline-degrading bacterium, Arthrobacter nicotianae OTC-16

Genomic insights into the antibiotic resistance pattern of the tetracycline-degrading bacterium, Arthrobacter nicotianae OTC-16

Biodegradation of Tetracycline Antibiotics by the Yeast Strain Cutaneotrichosporon dermatis M503

Genomic Insights Into Antibiotic Resistance Pattern of Tetracycline Degrading Bacterium Arthrobacter Nicotianae OTC-16

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