DEGRADATION OF ERGOTHIONEINE BY
            <i>ALCALIGENES FAECALIS</i>

Yanasugondha, Davi; Appleman, Maria D.

doi:10.1128/jb.74.3.381-385.1957

Cited by 25 publications

(7 citation statements)

References 15 publications

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“…There was also quite some variation in uptake between individuals, presumably reflecting variation in their expression of SLC22A4. Agrobacterium radiobacter (403) and other bacteria (404)(405)(406)(407)(408)(409) contain an ergothionase that degrades ERG to thiolurocanic acid (3-(1H-imidazol-5-yl)prop-2-enethioic S-acid) and trimethylamine, also implying that such cells possess one or more transporters for ERG. The thiolurocanic acid can be further degraded to glutamate (410) .…”

Section: Metabolism and Excretionmentioning

confidence: 99%

The biology of ergothioneine, an antioxidant nutraceutical

et al. 2020

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Ergothioneine (ERG) is an unusual thio-histidine betaine amino acid that has potent antioxidant activities. It is synthesised by a variety of microbes, especially fungi (including in mushroom fruiting bodies) and actinobacteria, but is not synthesised by plants and animals who acquire it via the soil and their diet, respectively. Animals have evolved a highly selective transporter for it, known as solute carrier family 22, member 4 (SLC22A4) in humans, signifying its importance, and ERG may even have the status of a vitamin. ERG accumulates differentially in various tissues, according to their expression of SLC22A4, favouring those such as erythrocytes that may be subject to oxidative stress. Mushroom or ERG consumption seems to provide significant prevention against oxidative stress in a large variety of systems. ERG seems to have strong cytoprotective status, and its concentration is lowered in a number of chronic inflammatory diseases. It has been passed as safe by regulatory agencies, and may have value as a nutraceutical and antioxidant more generally.

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Section: Metabolism and Excretionmentioning

confidence: 99%

The biology of ergothioneine, an antioxidant nutraceutical

et al. 2020

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“…Their chromatographic behaviour was indistinguishable from that of ergothioneine (Table 3). Other than ergothioneine, the only naturally occurring substance giving the Hunter reaction is thiolurocanic acid (Lawson, Morley & Woolf, 1951;Yanosugonolha & Appleman, 1957). This compound has, as yet, not been found in mammalian tissues and, though it gives the same colour as does ergothioneine with alkalinized sulphanilic acid, it does so without the 432 ERGOTHIONEINE AND THE CEREBELLAR FACTOR 433 liberation of trimethylamine.…”

Section: Presence Of Ergothioneine In the Central Nervous Systemmentioning

confidence: 99%

The presence of ergothioneine in the central nervous system and its probable identity with the cerebellar factor

Crossland¹,

Mitchell²,

Woodruff³

1966

The Journal of Physiology

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SUMMARY1. Ergothioneine has been detected and quantitatively estimated in the brains of mice, rats, guinea-pigs, rabbits, cats and sheep. It is present in the cerebellum in amounts ranging from about 3-0 ,ug/g in the cat to 10 ,ug/g in the guinea-pig. Amounts in the cerebral hemispheres are much smaller, ranging from < 041 ,ug/g in the cat to 1*6 gug/g in the guinea-pig.2. Large amounts of ergothioneine (about 30 ,ug/g) were found in the optic nerves of the rabbit. None was detected in the dorsal columns of the spinal cord of the same species.3. Ergothioneine has an excitatory action on the electrical activity of the cerebellum of the decerebrate rabbit and the excitatory actions of cerebellar extracts on this preparation are completely accounted for by their contained ergothioneine.4. It is concluded that the cerebellar factor of Crossland & Mitchell (1956) is identical with ergothioneine.

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“…Since both reactions proceeded most rapidly at pH 8.0, phosphate buffer at this pH was used routinely in subsequent experiments. In previous work (Yanasugondha and Appleman, 1957) Fig. 4.…”

Section: Resultsmentioning

confidence: 82%

DEGRADATION OF ERGOTHIONEINE BY CELL-FREE EXTRACTS OF ALCALIGENES FAECALIS

Kelly

Appleman

1961

J Bacteriol

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Yanasugondha and Appleman (1957) rein nutrient broth (Difco) plus yeast extract and ported that Alcaligenes faecalis is capable of then adapted by incubating overnight in phosadaptively degrading ergothioneine, with the phate buffer containing 1.0 Mlmole ergothioneine production of thiolurocanic acid and trimethylper ml. In a few experiments cells were grown in amine, followed by the disappearance of the a defined medium and then adapted by the same thiolurocanic acid, presumably due to its degra-procedure. dation to unknown products: The phosphate buffer used was a mixture of N(CH8)'.3+ 0.067 M KH2PO4 and 0.067 M Na2HPO4. In the N(CH,)t earlier experiments buffer at pH 7.0 was used. CH-C-CH2-CH-COO-Later, buffer at pH 8.0 was substituted.

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DEGRADATION OF ERGOTHIONEINE BY ALCALIGENES FAECALIS

Cited by 25 publications

References 15 publications

The biology of ergothioneine, an antioxidant nutraceutical

The biology of ergothioneine, an antioxidant nutraceutical

The presence of ergothioneine in the central nervous system and its probable identity with the cerebellar factor

DEGRADATION OF ERGOTHIONEINE BY CELL-FREE EXTRACTS OF ALCALIGENES FAECALIS

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