Degradation of Cytochrome P450 Heme and Lipid Peroxidation in Lead-Poisoned Rats

Penning, W; Scoppa, P.

doi:10.1007/978-1-4613-4124-6_51

Cited by 6 publications

(1 citation statement)

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“…However, after the initial week of exposure, it became clear that the high lead dose was not sublethal. In view of reports ( − ) that lead can cause oxidative stress to cell membranes of vertebrate species, and to shed light upon the possible mechanism for the observed toxicity, in-vitro experiments were also carried out to evaluate possible lead-induced oxidative injury to gill membranes.…”

Section: Introductionmentioning

confidence: 99%

Tissue Lead Concentration during Chronic Exposure of Pimephales promelas (Fathead Minnow) to Lead Nitrate in Aquarium Water

Spokas

Spur

Smith

et al. 2006

Environ. Sci. Technol.

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The fathead minnow is a useful species for evaluating the toxicity of wastewater effluents. While this fish is widely used for "survival" studies of metal toxicity, little or no work has been done on the tissue distribution of metals in fathead minnows. To determine the distribution of tissue lead, aquarium studies were conducted for several weeks with fish maintained in soft synthetic freshwater. Lead- (II) nitrate was added to three aquaria attaining concentrations of 20-30 ppb (aquarium B), 100-140 ppb (aquarium C), and roughly 200 ppb (aquarium D). Results were compared to controls (aquarium A). During the initial week, the majority of aquarium D fish died, whereas few deaths occurred in the other groups. Lead accumulation was dose- and tissue-dependent, with highest uptake by the gills. Gill concentrations of aquarium D fish averaged about 4-fold higherthan in skeleton or skin and muscle. In vitro, lead (2.5-25 ppm) caused dose-dependent reductions in the ratio of reduced glutathione/oxidized glutathione (GSH/GSSG) in gills incubated in physiological buffer. These findings demonstrate that fathead minnow gills bind and accumulate waterborne lead rapidly and preferentially and raise the possibility that gill lipid peroxidation contributes to lead toxicity at low water hardness.

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Section: Introductionmentioning

confidence: 99%