Deer Bone Oil Extract Suppresses Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Cells

Hyun-Min, Choi; Im, Suji; Park, Yooheon; Hong, Kyung Taek; Suh, Hyung Joo

doi:10.1248/bpb.b15-00952

Cited by 7 publications

(3 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, bone use has been limited in the field of food study due to its coarse and grainy texture, and bone is still mainly used as animal feed or plant fertilizer because of its low price (Buckley et al., 2012; Genisel et al, 2012). To improve the added value of animal bones, some of them are used for intensive processing, including extraction of bone protein (Dong et al., 2014; Song et al., 2016) and bone oil (Choi et al., 2016; Hagura et al., 2002), and preparation of natural hydroxyapatite (Heidari et al., 2018; Nam et al., 2019), gelatin (Li et al., 2013; Wang et al., 2016), and essence (You et al., 2020). However, the utilization of bone in these processes is limited, and more comprehensive use of bone still needs further study.…”

Section: Introductionmentioning

confidence: 99%

Effect of nanoprocessing on the physicochemical properties of bovine, porcine, chicken, and rabbit bone powders

et al. 2021

Food Science & Nutrition

View full text Add to dashboard Cite

The chemical composition and hardness of bovine bone, porcine bone, chicken bone, and rabbit bone were compared, as well as the influence of nanoprocessing on the physicochemical characteristics of these bone powders. A series of nanofabrication processes led to an increase in bone minerals and the loss of protein and fat. The hardness of softened bovine bone was still the largest, whereas chicken and rabbit bones were relatively soft. There were no significant differences in the functional groups between nanoscale bone powders. Overall, nanomachining significantly reduced and homogenized the bone particle size and improved the color and release rate of calcium ions of bone powders at the same time; these effects were different for several bones. Nanoscale rabbit bone had higher comminution efficiency, as well as satisfactory nutritional value, color, and product yield, which supports its strong development potential.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of nanoprocessing on the physicochemical properties of bovine, porcine, chicken, and rabbit bone powders

et al. 2021

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

“…Both of them did not have any cytotoxic effect on RAW 264.7 macrophages (Fig.1). According to Choi et al (2016), 26 the ability of deer bone methanol fraction in reducing NO production was a result of decreased regulation and expression mRNA from pro-inflammatory mediators, such as cyclooxygenase (COX-2), IL-12β, and IL-1β. The methanol fraction containing sugar played an essential role in lowering inflammatory responses by controlling pro-inflammatory cytokines and mediators.…”

Section: Extracts Of Deer Antlermentioning

confidence: 99%

The Effect of Rusa unicolor Antler Extracts from East Kalimantan in Bone Turnover Cell Models

Widyowati¹,

HARYADI²,

CHANG³

et al. 2019

tjps

View full text Add to dashboard Cite

Objectives: Osteoporosis is a disease described by a skeletal degradation of bone tissue domination to increase risk of fracture. Traditionally, Rusa unicolor antler from East Kalimantan is used to treat many kinds of diseases, and one of them is disease related to bone turnover. The present research aims to analyze the effects of 70% ethanol and aqueous extracts of Rusa unicolor antler from East Kalimantan on nitric oxide inhibition, osteoblast differentiation, and mineralization related to bone turnover. Materials and methods: The nitric oxide inhibition of the extracts in LPS-stimulated RAW 264.7 cells were evaluated by Griess reagent while osteoblast differentiations of extracts were evaluated by measuring alkaline phosphatase in p-nitrophenyl phosphate and their mineralization was determined using Alizarin Red Staining method. Results: The 70% ethanol and aqueous extracts inhibited cells inflammation (40% and 80%) and stimulated osteoblast differentiation (65% and 52%), respectively. In mineralization test, the aqueous extract showed two times higher effect than that of 70% ethanol extract. u n c o r r e c t e d p r o o f Conclusion: The extracts can be considered to successfully reduce expression of inflammatory markers on osteoblasts and maintain osteoblast functions.

show abstract

“…Several studies have shown the therapeutic effect of emu oil in osteoarthritis. 14,15) In recent years, we previously reported anti-inflammatory effect of deer bone oil extract (DBOE). 16) However, anti-osteoarthritis of deer bone oil extract has not been confirmed, and its active component has not been analyzed.…”

mentioning

confidence: 99%

Protective Effect of Deer Bone Oil on Cartilage Destruction in Rats with Monosodium Iodoacetate (MIA)-Induced Osteoarthritis

Hyun-Min

Im²,

Park

et al. 2016

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

The anti-osteoarthritic activity of the methanol fraction of deer bone oil extract (DBO-M) was evaluated in interleukin (IL)-1β-inflamed primary rabbit chondrocytes and in rats with monosodium iodoacetate (MIA)-induced osteoarthritis. The active compound in DBO-M was analyzed using a direct infusion liquid chromatography quadrupole (LCQ) ion-trap electrospray ionization (ESI)-mass spectrometer (MS). DBO-M significantly suppressed the IL-1β-induced sulfated-glycosaminoglycan (s-GAG) release from chondrocyte, and lowered mRNA levels of the collagen-degrading enzymes matrix metalloproteinase (MMP)-1 and MMP-3 in a dose-dependent manner. Upon treatment with high doses of DBO-M, the levels of IL-1β, tumor necrosis factor (TNF)-α, and IL-6 decreased by around 40, 70, and 50%, respectively, compared to the control in the serum of rats with MIA-induced osteoarthritis. Bone volume fraction (BV/TV) and trabecular thickness (Tb.Th) increased by over 40% in rats treated with DBO-M compared to the values reported for the MIA-treated control group, while trabecular separation (Tb.Sp) showed a significant decrease (ca. 38%), as confirmed through micro-computed tomography (CT) analysis of MIA-induced destruction of articular bones. Furthermore, direct infusion ESI-MS analysis showed that DBO-M contains gangliosides, which are glycosphingolipids with monosialic acid (GM3), as a major compound. Our results suggest that DBO-M effectively improves MIA-induced osteoarthritis by suppressing inflammatory responses, and that gangliosides could be one of the DBO-derived anti-inflammatory components.

show abstract

Deer Bone Oil Extract Suppresses Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Cells

Cited by 7 publications

References 36 publications

Effect of nanoprocessing on the physicochemical properties of bovine, porcine, chicken, and rabbit bone powders

Effect of nanoprocessing on the physicochemical properties of bovine, porcine, chicken, and rabbit bone powders

The Effect of Rusa unicolor Antler Extracts from East Kalimantan in Bone Turnover Cell Models

Protective Effect of Deer Bone Oil on Cartilage Destruction in Rats with Monosodium Iodoacetate (MIA)-Induced Osteoarthritis

Contact Info

Product

Resources

About