Protective Effect of Deer Bone Oil on Cartilage Destruction in Rats with Monosodium Iodoacetate (MIA)-Induced Osteoarthritis

Abstract

The anti-osteoarthritic activity of the methanol fraction of deer bone oil extract (DBO-M) was evaluated in interleukin (IL)-1β-inflamed primary rabbit chondrocytes and in rats with monosodium iodoacetate (MIA)-induced osteoarthritis. The active compound in DBO-M was analyzed using a direct infusion liquid chromatography quadrupole (LCQ) ion-trap electrospray ionization (ESI)-mass spectrometer (MS). DBO-M significantly suppressed the IL-1β-induced sulfated-glycosaminoglycan (s-GAG) release from chondrocyte, and lowered mRNA levels of the collagen-degrading enzymes matrix metalloproteinase (MMP)-1 and MMP-3 in a dose-dependent manner. Upon treatment with high doses of DBO-M, the levels of IL-1β, tumor necrosis factor (TNF)-α, and IL-6 decreased by around 40, 70, and 50%, respectively, compared to the control in the serum of rats with MIA-induced osteoarthritis. Bone volume fraction (BV/TV) and trabecular thickness (Tb.Th) increased by over 40% in rats treated with DBO-M compared to the values reported for the MIA-treated control group, while trabecular separation (Tb.Sp) showed a significant decrease (ca. 38%), as confirmed through micro-computed tomography (CT) analysis of MIA-induced destruction of articular bones. Furthermore, direct infusion ESI-MS analysis showed that DBO-M contains gangliosides, which are glycosphingolipids with monosialic acid (GM3), as a major compound. Our results suggest that DBO-M effectively improves MIA-induced osteoarthritis by suppressing inflammatory responses, and that gangliosides could be one of the DBO-derived anti-inflammatory components.

Comparison of the therapeutic effect of amniotic fluid stem cells and their exosomes on monoiodoacetate‐induced animal model of osteoarthritis

“…• Group 1 (four animals): OA joint, treated with 2 mg/50 μl/knee MIA intra-articular injection (MIA, Sigma Aldrich, Milano), a concentration comparable with the literature. 19,20 • Group 2 (eight animals): OA joint + CELLS, treated with 5 × 10 5 AFSC 3 weeks after MIA. • Group 3 (eight animals): OA joint + EXO, treated twice (10 days of distance) with 100 μg exosomes 3 weeks after MIA.…”

Pink1-Mediated Chondrocytic Mitophagy Contributes to Cartilage Degeneration in Osteoarthritis

“…Osteoarthritis (OA) is a complex disease involving the whole synovial joint tissue. OA is the most common form of arthritis, characterized by cartilage destruction, as well as synovial inflammation and subchondral bone remodeling [1,2]. Animal models of OA have greatly improved our understanding of its pathogenesis and treatment.…”

Nutraceutical supplements in management of pain and disability in osteoarthritis: a systematic review and meta-analysis of randomized clinical trials

“…Deer horn extract has been considered as a noteworthy health restorative in traditional medicine amongst several Asian countries 67 . Oily DBE and CPC were recently revealed to have anti-inflammatory properties and reduce the morphological deviations related with osteoarthritic cartilage damage in animal models of OA 68,69 .…”