The Effect of Rusa unicolor Antler Extracts from East Kalimantan in Bone Turnover Cell Models

Widyowati, Retno; HARYADI, Dewi Melani; CHANG, Hsin-I; SURYAWAN, IPG Ngurah; UTAMA, Agung Widi

doi:10.4274/tjps.57805

Cited by 3 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The absorbance was ruminated at 570 nm by an enzyme-linked immunosorbent assay (ELISA) reader. All experiments were performed in triplicate, with the relative cell viability (%) declared as a portion relative to the unprocessed control cells [15,16].…”

Section: Cell Viability Assaymentioning

confidence: 99%

“…After incubation, the cell lysates were examined for ALP by adding 200 µL of p-nitrophenyl phosphate (PNPP) and diethanolamine buffer into each well for a period of 30 min and at room temperature. The 50 µL/well stop solution was inserted to stop the reaction while ELISA reader at 405 nm was applied to measure the absorbance [15,16].…”

Section: Differentiation Of Cellular Alkaline Phosphatase Activity (Alp)mentioning

confidence: 99%

See 1 more Smart Citation

Exploration of several plants from Baung Forest on bone formation cell models

Widyowati

Purwitasari

Oktarina

et al. 2021

Journal of Basic and Clinical Physiology and Pharmacology

Self Cite

View full text Add to dashboard Cite

Objectives Osteoporosis is an ailment described by a skeletal degradation of bone skeletal dominating to increases the chance of fracture. In order to find out the bone formation agents from Baung Forest plants, this research analyzed the effects of 96% ethanol extract of several plants from Baung Forest on antioxidant activity and the effect of osteoblast differentiation-related to the bone formation on the most potent extract. Methods The antioxidant effect and osteoblast differentiation of 96% ethanol extracts were evaluated by measuring DPPH scavenging and alkaline phosphatase in p-nitrophenyl phosphate effects by the enzyme-linked immunosorbent assay (ELISA) reader method, respectively. Results The 96% ethanol extract of Elaeocarpus serratus L. from Baung Forest had the strongest DPPH radical scavenging as anti oxidant (82.17%) and stimulated osteoblast differentiation (116%). Then, this extract had been fractionated based on polarity to become hexane, ethyl acetate, butanol, and aqueous fractions. All the fractions stimulated their alkaline phosphatase (ALP) activity to 138.11 ± 9.72%, 108 ± 5.05%, 148.56 ± 8.47, and 144.58 ± 1.04, respectively. Conclusions The extract and fractions of E. serratus L. can successfully inhibit DPPH radical scavenging value and increase ALP activities as markers of osteoblast functions.

show abstract

Section: Cell Viability Assaymentioning

confidence: 99%

Section: Differentiation Of Cellular Alkaline Phosphatase Activity (Alp)mentioning

confidence: 99%

Exploration of several plants from Baung Forest on bone formation cell models

Widyowati

Purwitasari

Oktarina

et al. 2021

Journal of Basic and Clinical Physiology and Pharmacology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some clinical evidences recommend a role for phytoestrogen in the therapy of osteoporosis [12][13][14][15]. Previous studies showed that 70% ethanol and aqueous extracts of deer antler from East Kalimantan increased the alkaline phosphatase (ALP) and mineralization activities of 7F2 cell that is an osteoblast cell lines [16,17]. These extracts contain several major constituents such as protein, lipids, ash, calcium, collagen, chondroitin sulfate and glucosamine [18,19].…”

Section: Introductionmentioning

confidence: 99%

The effect of deer antler from East Kalimantan to increase trabecular bone density and calcium levels in serum on osteoporotic mice

Widyowati

Suciati

Haryadi

et al. 2021

Journal of Basic and Clinical Physiology and Pharmacology

View full text Add to dashboard Cite

Objectives Glucocorticoid-induced osteoporosis (dexamethasone) is a primary cause of secondary osteoporosis by the decreasing formation and increasing resorption activities. Previously, the in vitro study showed that 70% ethanol and aqueous extract of deer antler have increased alkaline phosphatase in osteoblast cell that known as marker of bone formation. The mind of this study is to analyze the effect of deer antlers in increasing the bone trabecular density of osteoporosis-induced male mice. Methods This study used a post-test control group design. A total of 54 healthy male mice were randomly divided to nine groups, i.e., healthy control, osteoporotic, positive control, 70% ethanol (4, 8, and 12 mg/kg BW), and aqueous extracts (4, 8, and 12 mg/kg BW) of deer antler groups. All of the interventions were given 1 mL of test sample for 4 weeks orally. The bone densities were determined using histomorphometry by Image J and Adobe Photoshop. The statistical data were performed using SPSS 23 and statistical significance was set at p<0.05. Results The results showed that alendronate group, 70% ethanol, and aqueous extract groups increased bone density and calcium levels in serum (p<0.05) compared to osteoporotic group in dose dependent manner. It indicated that 70% ethanol and aqueous extract of deer antler stimulating bone turnover and aqueous extract showed the highest. Conclusions Dexamethasone induction for 4 weeks caused osteoporotic mice and the administration of 70% ethanol and aqueous extracts of deer antler from East Kalimantan increased trabecular bone density and calcium levels in dose dependent manner.

show abstract