Decreased responsiveness and development of activation markers of PLTs stored in plasma

Curvers, Joyce; Pampus, E.C.M. van; Feijge, Marion A.H.; Rombout‐Sestrienkova, Eva; Giesen, Peter; Heemskerk, Johan W. M.

doi:10.1111/j.0041-1132.2004.00628.x

Cited by 56 publications

(94 citation statements)

References 39 publications

(43 reference statements)

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“…P-selectin increased with time as shown by us and others [6,13,31]. PS exposure increased only minimally during the normal shelf-life of PCs but by day 12, the exposure had increased significantly.…”

Section: Discussionsupporting

confidence: 60%

“…The collagen beads did not change in percentage of beads with adhering platelets, but did increase the amount of platelets adhering to each bead during the first 7 days of storage. There are few studies on platelet adhesion during storage and they vary in the method of platelet preparation, storage length and storage media, making direct comparisons difficult, as all these factors affect platelet function [6,7,[9][10][11]. Furthermore these studies have tested platelets in presence of added red blood cells which could also influence their results.…”

Section: Discussionmentioning

confidence: 99%

“…The GPIIb/IIIa receptor is responsible for platelet binding to fibrinogen and aggregation. Several studies have shown a well preserved GPIIb/IIIa expression during storage [6,21,22] but GPIIb/IIIa have also been reported to increase [23] and the receptor can exist in an activated or non-activated form, thus further complicating interpretations. Analysis of the active conformation of GPIIb/IIIa was not assessed in our study, which would have been of interest since there are studies reporting that the GPIIb/IIIa receptor undergoes changes with storage [2,24], which might be one explanation to the increased platelet binding to fibrinogen beads observed in our study.…”

Section: Discussionmentioning

confidence: 99%

“…PS exposure increased only minimally during the normal shelf-life of PCs but by day 12, the exposure had increased significantly. Others have also shown an increase in PS exposure during storage, but to various degrees [6,16,18,30], the consequences of these apoptotic features during storage are largely unknown. Our results are in accordance with the results by Leytin et al [32] that platelet activation is triggered before exposure of apoptotic features and our results support the hypothesis that apoptosis is triggered beyond the current shelf-life [1,30].…”

Section: Discussionmentioning

confidence: 99%

“…One reason is that current available methods are time consuming, complicated to use, and require large sample volumes. The adhesion capacity of stored platelets has been studied using perfusion chambers [6][7][8] or cone and-plate devices [9,10]. These methods require the presence of red blood cells, which complicates the analysis of PCs and introduces a potential source of non-specific and unpredictable effects [8,9,11].…”

Section: Introductionmentioning

confidence: 99%

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Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

et al. 2014

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The aim of the present study was to set up and evaluate a novel method for studies of platelet adhesion and activation in blood and platelet suspensions such as platelet concentrate (PC) samples using protein-coated polystyrene beads and flow cytometry. To demonstrate its usefulness, we studied PCs during storage. PCs were prepared by apheresis technique (N = 7).Metabolic variables and platelet function was measured on day 1, 5, 7 and 12 of storage.Spontaneous and TRAP-6-induced adhesion to fibrinogen-and collagen-coated beads was analysed by flow cytometry. P-selectin and phosphatidyl serine (PS) expression was assessed on platelets bound to beads as well as on non-adherent platelets. Platelet adhesion to fibrinogen beads had increased by day 12 and adhesion to collagen beads at day 7 of storage (p < 0.05). TRAP-6 stimulation significantly increased the platelet adhesion to fibrinogen beads (p < 0.05) as well as the P-selectin and PS exposure on platelets bound to beads (p < 0.01) during the first 7 days of storage, but by day 12, significant changes were no longer induced by TRAP-6 stimulation.We demonstrate that our adhesion assay using protein-coated polystyrene beads can be used to assess the adhesion properties of platelets during storage without the addition of red blood cells. Therefore it may offer a useful tool for future studies of platelet adhesive capacity in transfusion medicine and other settings.3

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

et al. 2014

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Usefulness of delta value of platelet parameters on ADVIA 120 for the functional reactivity of stored platelets

Park

Lim

Cho³

2010

Clinical Laboratory Analysis

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An agonist-induced expression of CD62P by flow cytometry analysis for evaluating platelet functional reactivity has some disadvantages. We investigated the usefulness of platelet parameters by ADVIA 120 to predict an agonist-induced expression of CD62P in stored platelets. The CD62P expression by flow cytometry and the platelet parameters by ADVIA 120 were studied in samples from 27 platelet pheresis products. Delta (D) values were calculated as the degree of change of the platelet parameters studied with or without adenosine 5 0 -diphosphate sodium (ADP) stimulation. The CD62P expression of the ADP-activated platelets were correlated with the Dplatelet count (r 5 0.517) in the short-term storage group (within 10 hr from preparation), with the platelet component distribution width (PCDW) without ADP (r 5 À0.744) and the DPCDW (r 5 À0.755) in the long-term storage group (after 10 hr from preparation). Therefore, the delta values of platelet parameters on ADVIA 120 analysis in platelets between with and without ADP stimulation could be useful as a simple predictor for the functional reactivity of stored platelets.

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Flow Cytometry

Carubbi¹,

Masselli²,

Vitale³

2017

Platelets in Thrombotic and Non-Thrombotic Disorders

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Decreased responsiveness and development of activation markers of PLTs stored in plasma

Abstract: PLTs stored in plasma develop surface activation markers but, simultaneously, show markedly decreased responsiveness toward physiologic agonists. The plasma contains high coagulant activity, which is no longer PLT (activation)-dependent.

Cited by 56 publications

References 39 publications

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

Usefulness of delta value of platelet parameters on ADVIA 120 for the functional reactivity of stored platelets

Flow Cytometry

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