Decreased Galectin-3 and -7 Expressions in Old-Aged Skin and Their Differential Expression in Skin Equivalents

Choi, Jee Woong; Nam, Kyung Mi; Choi, Hyo Geun; Lee, Dong Hun; Huh, Chang Hun; Park, Kyung Chan

doi:10.5021/ad.2018.30.3.375

Cited by 4 publications

(5 citation statements)

References 9 publications

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“…Our findings with regard to the Gal 7 expression pattern of human adult epidermis are in agreement with those reported by Akimoto et al (28), who suggested that, in view of Gal 7 expression in both the epidermis and in dermal components, this galectin is important for cell-cell contact and/or adhesion in the epidermis and for cell-extracellular matrix interactions in the dermis. Our findings are also in line with the results of a study by Choi et al (34), who provided evidence suggesting that Gal 7 is a sensitive marker for differentiated and active keratinocytes of human adult epidermis, in which its expression reveals a progressive age-dependent decrease.…”

Section: Discussionsupporting

confidence: 93%

The expression pattern of galectins during human epidermal morphogenesis

Pasmatzi

Papadionysiou

Monastirli

et al. 2020

Acta Dermatovenerologica Alpina Pannonica Et Adriatica

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Introduction: Galectins constitute a phylogenetically conserved family of proteins with high binding affinity for glycoconjugates bearing β-galactoside residues. Surprisingly, knowledge of the expression pattern of galectins during human epidermal morphogenesis is very limited. Methods: Fifty-eight biopsy skin specimens obtained from human embryos and 10 biopsy specimens obtained from healthy adult volunteers were processed for immunohistochemistry using a panel of antibodies against galectins 1, 3, 7, and 9. Results: Fetal human epidermis was devoid of any galectin 1 immunoreactivity, whereas clear-cut changes were found in the galectin 3 immunoreactivity of fetal epidermis with advancing gestational age. The expression pattern of galectins 7 and 9 remained constant at all stages of gestation. Conclusions:The changes in galectin 3 immunoreactivity of human fetal epidermis with advancing gestational age, which are reported here for the first time, suggest that this galectin and its ligands may be implicated in the molecular events underlying human epidermal morphogenesis. It remains to be elucidated in future investigations whether the expression pattern of galectins, particularly that of galectin 3, in the developing human epidermis reveals alterations in fetuses with inherited cutaneous disorders that may be important for prenatal diagnosis of these disorders.

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Section: Discussionsupporting

confidence: 93%

The expression pattern of galectins during human epidermal morphogenesis

Pasmatzi

Papadionysiou

Monastirli

et al. 2020

Acta Dermatovenerologica Alpina Pannonica Et Adriatica

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“…We show that dermal biglycan is also subjected to modification by photoageing which may impair functionality within regions (Figure 3c) corresponding to three of its leucine‐rich repeats (Figure ). Although reductions in epidermal galectin‐7 expression (important in wound repair) was shown recently in intrinsically aged skin (Choi et al, 2018), we show that dermal galectin‐7 is additionally susceptible to photoageing (Figure 3d).…”

Section: Resultssupporting

confidence: 43%

“…Protein identities contained within sub-categories are also listed (Figure S5). Although reductions in epidermal galectin-7 expression (important in wound repair) was shown recently in intrinsically aged skin (Choi et al, 2018), we show that dermal galectin-7 is additionally susceptible to photoageing (Figure 3d). Epidermal K2 and K10 had evidence of photoageing-dependent structural modifications evidenced by peptide yield differences (Figure 3e,f) within the coil domains (Figure S5).…”

Section: Peptide Location Fingerprinting Reveals Regional Fluctuation...supporting

confidence: 38%

Peptide location fingerprinting reveals modification‐associated biomarker candidates of ageing in human tissue proteomes

et al. 2021

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“…Galectin-7 plays a key role in epidermal cell migration and wound re-epithelialisation (32). Although reductions in epidermal galectin-7 expression was shown recently in intrinsically aged skin (33), our study demonstrates that dermal galectin-7 is additionally susceptible to modifications as a consequence of photoageing.…”

Section: Resultsmentioning

confidence: 42%

Peptide location fingerprinting reveals modification-associated biomarkers of ageing in human tissue proteomes

Ozols

Eckersley

Mellody

et al. 2020

Preprint

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Although dysfunctional protein homeostasis (proteostasis) is a key factor in many age-related diseases, the untargeted identification of structural modifications in proteins remains challenging. Peptide location fingerprinting is a proteomic analysis technique capable of identifying structural modification-associated differences in mass spectrometry (MS) datasets of complex biological samples. A new webtool (Manchester Peptide Location Fingerprinter), applied to photoaged and intrinsically aged skin proteomes, can relatively quantify peptides (spectral counting) and map statistically significant differences to regions within protein structures. New photoageing biomarkers were identified in multiple proteins including matrix components (collagens and proteoglycans), oxidation and protease modulators (peroxiredoxins and SERPINs) and cytoskeletal proteins (keratins). Crucially, for many extracellular biomarkers, structural modification-associated differences were not correlated with relative abundance (by ion intensity). By applying peptide location fingerprinting to published MS datasets, (identifying biomarkers including collagen V and versican in ageing tendon) we demonstrate the potential of the MPLF webtool to discover novel biomarkers.

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Decreased Galectin-3 and -7 Expressions in Old-Aged Skin and Their Differential Expression in Skin Equivalents

Cited by 4 publications

References 9 publications

The expression pattern of galectins during human epidermal morphogenesis

The expression pattern of galectins during human epidermal morphogenesis

Peptide location fingerprinting reveals modification‐associated biomarker candidates of ageing in human tissue proteomes

Peptide location fingerprinting reveals modification-associated biomarkers of ageing in human tissue proteomes

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