Decomposition of sugarcane bagasse with lignocellulose-derived thermotolerant and thermoresistant Penicillia and Aspergilli

Boonyuen, Nattawut; Manoch, Leka; Luangsa-ard, Janet Jennifer; Piasai, Onuma; Chamswarng, Chiradej; Chuaseeharonnachai, Charuwan; Ueapattanakit, Jureerat; Arnthong, Jantima; Sri‐indrasutdhi, Veera

doi:10.1016/j.ibiod.2014.04.013

Cited by 19 publications

(9 citation statements)

References 59 publications

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“…In order to solve this challenge, it is important to search for efficient and economically viable enzyme-producing microorganisms (Moretti et al, 2012;Santos et al, 2015). The hydrolytic enzymes are mainly produced by filamentous fungi and several studies have been performed to select strains with a high rate of enzyme production, especially hemicellulases and cellulases (Boonyuen et al, 2014;Santos et al, 2015). Among the cellulases, β-glucosidases or cellobiases (EC 3.2.1.21) have the function of ending the hydrolysis of cellulose, releasing glucose from cellobioses and cellooligosaccharides previously generated by the action of other enzymes of the cellulolytic complex, which are fundamental for the success of the saccharification process (Murphy et al, 2013;Watanabe and Tokuda, 2010).…”

Section: Introductionmentioning

confidence: 99%

Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

Rodrigues

Pereira

Santos

et al. 2017

Industrial Crops and Products

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This study evaluated the synergistic efficiency of crude enzymatic extracts (CEE) produced by fungal strains Aspergillus fumigatus SCBM6 (AFE) and Aspergillus niger SCBM1 (ANE) in the hydrolysis of alkali pretreated sugarcane bagasse (2% NaOH (w v −1), ∼100°C, 4 h and liquid-to-solid ratio of 25 (v w −1)). CEEs were produced by solid-state fermentation (SSF) and presented high concentration of β-glucosidase (AFE = 78.4 U g −1 and ANE = 43.02 U g −1), β-xylosidase (ANE = 78.0 U g −1) and xylanase (AFE = 1774.5 U g −1 and ANE = 3289.6 U g −1). A 2 4 full design of experiments was used to optimize the hydrolysis of pretreated bagasse as a function of time, temperature, amount of pretreated bagasse and AFE-to-ANE ratio. After hydrolysis optimization, the maximum concentration of total reducing sugars (TRS) was 21.68 g L −1. It was also noticed a significant production of xylose (135.59 mg) and arabinose (58.10 mg) per gram of hemicelluloses and a slight production of glucose (20.18 mg) per gram of cellulose. As fungi consortium were excellent producers of hemicellulases, it is suggested that they could be used for pentoses saccharification and production of ethanol via C-5 fermentation, xylitol, biopolymers, among other bioproducts.

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Section: Introductionmentioning

confidence: 99%

Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

Rodrigues

Pereira

Santos

et al. 2017

Industrial Crops and Products

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“…Boonyuen N et al observed that microorganisms could decompose cellulose at a lower rate than hemicellulose, but higher than lignin. [48] As a comparison, lignin content suffered a more obvious increase than cellulose content ( Fig. 3a and 3b).…”

Section: Data Processing and Statistical Analysismentioning

confidence: 87%

The variability and causes of organic carbon retention ability of different agricultural straw types returned to soil

Wang

Zhang

et al. 2016

Environmental Technology

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Retaining the organic carbon (C) content of agricultural straw when returned to soil is restricted by rapid decomposition. In order to clarify the difference in returned straw decomposition and the causes, and to develop a straw returning mode with high-efficiency of organic C accumulation, the decomposition processes of corn, soybean, rice and wheat straws were systematically studied in fields. When returned in situ (the original planting area), the C in soybean straw was decomposed most quickly with a decomposition constant of 0.00542 d, but wheat straw showed a longer retention in soil with 0.00303 d. However, for ex situ return of all straw in one area away from in situ return, soybean straw was decomposed most slowly (0.00452 d) and wheat straw more quickly (0.00652 d). The sequence of C decomposition rate in 270 d was soybean > corn > rice > wheat (in situ) and corn > wheat > rice > soybean (ex situ). Both surrounding soil and straw nature were important factors influencing the decomposition rate. The farmland with rice and wheat rotation retained more C from returned straws due to its high moisture and low nitrogen (N) content, while the soybean field was a contrast. Soybean straw had a low decomposition rate after ex situ return due to its low N content and high C/N ratio. The farmland of wheat-rice rotation combined with soybean straw ex situ return may develop into a field of higher C retention ability.

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“…The viability of lignocellulosic biofuel industry will depend on development of cheaper and compatible enzyme cocktail for release of sugars from saccharification of biomass feedstock (Boonyuen et al, 2014 ). In this study, both xylanase and cellulase enzyme present cultural filtrate of Fusarium sp.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Xylanase Production through Response Surface Methodology by Fusarium sp. BVKT R2 Isolated from Forest Soil and Its Application in Saccharification

Ramanjaneyulu

Reddy

2016

Front. Microbiol.

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Xylanses are hydrolytic enzymes with wide applications in several industries like biofuels, paper and pulp, deinking, food, and feed. The present study was aimed at hitting at high yield xylanase producing fungi from natural resources. Two highest xylanase producing fungal isolates—Q12 and L1 were picked from collection of 450 fungal cultures for the utilization of xylan. These fungal isolates—Q12 and L1 were identified basing on ITS gene sequencing analysis as Fusarium sp. BVKT R2 (KT119615) and Fusarium strain BRR R6 (KT119619), respectively with construction of phylogenetic trees. Fusarium sp. BVKT R2 was further optimized for maximum xylanase production and the interaction effects between variables on production of xylanase were studied through response surface methodology. The optimal conditions for maximal production of xylanase were sorbitol 1.5%, yeast extract 1.5%, pH of 5.0, Temperature of 32.5°C, and agitation of 175 rpm. Under optimal conditions, the yields of xylanase production by Fusarium sp. BVKT R2 was as high as 4560 U/ml in SmF. Incubation of different lignocellulosic biomasses with crude enzyme of Fusarium sp. BVKT R2 at 37°C for 72 h could achieve about 45% saccharification. The results suggest that Fusarium sp. BVKT R2 has potential applications in saccharification process of biomass.

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Decomposition of sugarcane bagasse with lignocellulose-derived thermotolerant and thermoresistant Penicillia and Aspergilli

Cited by 19 publications

References 59 publications

Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

The variability and causes of organic carbon retention ability of different agricultural straw types returned to soil

Optimization of Xylanase Production through Response Surface Methodology by Fusarium sp. BVKT R2 Isolated from Forest Soil and Its Application in Saccharification

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