Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

Rodrigues, Patrísia de Oliveira; Pereira, Joelma; Santos, Douglas Queiroz; Gurgel, Leandro Vinícius Alves; Pasquini, Daniel; Baffi, Milla Alves

doi:10.1016/j.indcrop.2017.08.031

Cited by 30 publications

(9 citation statements)

References 46 publications

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“…Cellobiose is a stronger inhibitor than glucose and its accumulation causes feedback inhibition against most cellobiohydrolases and endoglucanases, and thus it becomes important to supply enough β-glucosidases to hydrolyze cellobiose to glucose in order to eliminate such inhibition (Wang and Lu, 2016). The sufficient amount of β-glucosidase may lead to alleviation of the feedback inhibition by cellobiose (de Oliveira Rodrigues et al, 2017). Other accessory enzymes like hemicelluloses, esterases, arabinofuranosidases, laccases remove the physical protective covering of the cellulase microfibrils thereby, improving the enzyme accessibility (Gonçalves et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

et al. 2018

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Multiple enzymes are required for efficient hydrolysis of lignocellulosic biomass and no wild type organism is capable of producing all enzymes in desired levels. In this study, steam explosion of wheat straw was carried out at pilot scale and a synthetic enzyme mixture (EnzMix) was developed by partially replacing the cellulase with critical dosages of commercially available accessory enzymes (β-glucosidase, xylanase and laccase) through central composite design. Highest degree of synergism (DS) was observed with β-glucosidase (1.68) followed by xylanase (1.36). Finally, benchmarking of EnzMix (Celluclast, β-glucosidase and xylanase in a protein ratio of 20.40: 38.43: 41.16, respectively) and other leading commercial enzymes was carried out. Interestingly, hydrolysis improved by 75% at 6 h and 30% at 24 h, respectively in comparison of control. By this approach, 25% reduction in enzyme dosage was observed for obtaining the same hydrolysis yield with opitimized enzyme cocktail. Thus, development of enzyme cocktail is an effective and sustainable approach for high hydrolysis efficiency.

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Section: Introductionmentioning

confidence: 99%

Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

et al. 2018

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“…Entre os pré-tratamentos (PT) físico-mecânicos, o mais comum é a moagem ou trituração da biomassa que reduz o tamanho das partículas, tendo como principais vantagens a redução das regiões cristalinas da celulose e o aumento da área superficial do substrato (RAMOS et al, 2015;WANDERLEY et al, 2013 HILDÉN et al, 2000). Assim, diversos estudos têm sido realizados utilizando fungos filamentosos para produção de enzimas por fermentação em estado sólido (FES) utilizando resíduos lignocelulósicos, como o próprio bagaço de cana ( MORETTI et al, 2012;BANSAL et al, 2014;DE CASSIA PEREIRA et al, 2015;RODRIGUES et al, 2017;DU et al, 2018;). Grande parte desses trabalhos apresentam a produção de coquetéis enzimáticos por fungos cultivados isoladamente (DA CUNHA et al, 2018;LOPEZ-RAMIREZ et al, 2018;.…”

Section: Produção De Etanol De 1ª E 2ª Geraçãounclassified

“…Os processos catalisados por enzimas são mais vantajosos em sacarificações de biomassa lignocelulósica, devido à baixa formação de inibidores e degradação de açúcares por requererem condições mais amenas de temperatura, pH e pressão atmosférica (ZABED et al, 2016). Assim, a bioconversão enzimática de bagaço de cana-de-açúcar tem se destacado em relação à hidrólise ácida (DE CASSIA PEREIRA et al, 2015;DU et al, 2018;MORETTI et al, 2012;RODRIGUES et al, 2017). Para maximizar a hidrólise, além de um pré-tratamento químico, um consórcio otimizado de enzimas deve ser aplicado, de acordo com a composição da lignocelulose em termos de lignina, hemiceluloses e celulose (ROCHA et al, 2015).…”

Section: Trametes Versicolor 561unclassified

“…Assim como diferentes ascomicetos, como as espécies do gênero Aspergillus, também são bons produtores principalmente de celulases e hemicelulases (DOS SANTOS et al, 2019;RODRIGUES et al, 2017).…”

Section: Introdução E Justificativaunclassified

“…e A. fumigatus SCBM6 (Fu), respectivamente, previamente determinadas porRodrigues et al (2017), apenas nos extratos produzidos por essas culturas isoladas. Nos demais extratos, a temperatura dos ensaios foi padronizada em 50ºC, em uma mistura de reação contendo 50 µL de Uma unidade de atividade enzimática foi definida como a quantidade de enzima necessária para liberar 1 µmol de xilose por minuto de reação, a partir de uma curva padrão de xilose.5.4.6.…”

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Uso de coquetéis lignocelulolíticos produzidos por consórcio fúngico na sacarificação de bagaço de cana-de-açúcar e produção de etanol

Rodrigues¹

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The present study evaluated the production of lignocellulolytic cocktails by solid-state fermentation (SSF), using sugarcane bagasse and wheat bran as substrates and the fungal strains Aspergillus niger SCBM1 (Ni), Aspergillus fumigatus SCBM6 (Fu), Ganoderma lucidum 601 (Ga), Trametes versicolor 561 (Tr) and Pleurotus ostreatus PLO6 (Pl) in monocultures and/or in consortia. The crude extracts produced were evaluated in the hydrolysis of sugarcane bagasse pretreated by autohydrolysis (AH) and by autohydrolysis followed by alkaline pretreatment (NaOH) (AH-Soda). The enzymatic cocktails that presented the best yields in terms of fermentable sugars were selected and evaluated in new hydrolyses experiments with high loads of solids and with addition of the surfactant Triton X-100. At last, alcoholic fermentations of the hydrolysates containing the highest glucose concentrations were carried out. In the SSF performed with isolated strains, the fermentation that used A. niger SCBM1 as inoculum (Ni-F1 extract) was the most outstanding in the production of enzymes, with maximum activities for endoglicanae (82.70 U/g), exoglicanae (80.48 U/g), βxylosidase (145.01 U/g) and manganese peroxidase (3.38 U/g). For the SSF performed in co-culture of fungal strains, the extracts produced by consortia between Ni+Fu+Pl (F9), Ni+Tr+Pl (F6) and Ni+Ga+Pl (F10) presented the highest productions of cellulases and hemicellulases, mainly β-glucosidase (104.70, 171.09 and 106.10 U/g), β-xylosidase (89.72, 139.99 and 121.02 U/g) and xylanase (2582.38, 2267.96 and 1697.74 U/g), respectively. The crude cocktail produced from the consortium between Basidiomycetes (Tr+Ga+Pl) was the most efficient for laccase production (25.27 U/g). After the hydrolyses, the highest glucose concentrations were obtained in the hydrolysates of sugarcane bagasse pretreated by AH and AH-Soda using Ni extract (F1) with 10.78 and 10,32 g/L, Fu+Ga+Pl extract (F11) with 9.76 g/L (AH) and 10.60 g/L (AH-Soda) and Ni+Ga+Pl extract (F10) with 11.92 g/L in AH-Soda hydrolysate. For xylose, the highest release was obtained after F8 hydrolysis that used Ni+Fu+Tr extract in sugarcane bagasse pretreatment by AH (5.08 g/L). After alcoholic fermentation, 60.80 % (in relation to the theoretical maximum) of conversion was achieved in F1 (Ni hydrolysate), with a production of 3,20 g/L of ethanol and 58,15 % in F6 (3,08 g/L) of ethanol. In hydrolysis performed with high load of solids, the results obtained showed that the concentrations of total reducing sugars (TRS) increased from 15.54 to 44.84 g/L in Ni hydrolysate (F1), when the percentage of bagasse pretreatment by AH increased from 10 to 35 %. In the Ni+Tr+Pl (F6) hydrolysate, TRS concentration increased from 11.38 to 31.77 g/L under the same conditions. The addition of surfactant did not significantly influenced concentration of TRS in Ni hydrolysate (45.16 g/L). In the F6 hydrolysis, the TRS concentration increased from 30.94 to 48.16 g/L, suggesting that for this extract, the use of surfactant favored the enzymatic action. The...

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Multi‐enzymatic recovery of fungal cellulases (Aspergillus niger) through solid‐state fermentation of sugarcane bagasse

et al. 2021

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In this study, the optimum conditions for the multi‐enzymatic recovery of cellulases produced by Aspergillus niger were investigated using sugarcane bagasse. Two extraction methods were investigated: the two‐stage solid–liquid extraction (SLE) followed by ultrasound‐assisted extraction (UAE), and the single‐stage SLE. The ultrasound effects were evaluated using a Doehlert design, in which the pH (5.0–9.0) and sonication power (0.8–2.0 W ml−1) were independent variables. For the single‐stage SLE, temperature (25–45°C), time (10–60 min), and pH (5.0–9.0) were analyzed using the Box–Behnken design. Both processes were monitored to evaluate FPase, CMCase, and β‐glucosidase (U ml−1) activities. The maximum enzymatic activities (EA) obtained for FPase, CMCase, and β‐glucosidase in the SLE–UAE were 0.352, 0.321, and 1.412 U ml−1, respectively. Unlike in most previous studies, sonication was insignificant (p < 0.05) with respect to the enzymatic complex within the evaluated ranges. Moreover, sonication changed the EA when lower than 1.2 and higher than 1.6 W ml−1, mainly inhibiting the EA of β‐glucosidase. The single‐stage SLE was more effective than the two‐stage SLE–UAE, and the maximum EA values for FPase, CMCase, and β‐glucosidase were 0.354, 0.303, and 3.135 U ml−1, respectively. The single‐stage process was better because it consumed less energy, required simpler equipment, and provided higher efficiency in a shorter time. This study will improve diversified enzyme extraction from sugarcane bagasse, reduce enzyme production costs, and enhance bagasse utilization.

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Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

Cited by 30 publications

References 46 publications

Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

Uso de coquetéis lignocelulolíticos produzidos por consórcio fúngico na sacarificação de bagaço de cana-de-açúcar e produção de etanol

Multi‐enzymatic recovery of fungal cellulases (Aspergillus niger) through solid‐state fermentation of sugarcane bagasse

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