Deciphering the mechanism and structural features of polysorbate 80 during adsorption on PLGA nanoparticles by attenuated total reflectance – Fourier transform infrared spectroscopy

Abstract

Structural changes and adsorption mechanism of polysorbate 80 on PLGA nanoparticles by using novel extraction strategy and ATR-FTIR technique.

Inhibition of polyglutamine aggregation by SIMILAR huntingtin N‐terminal sequences: Prospective molecules for preclinical evaluation in Huntington's disease

“…These inhibitors can be tested now in cell and animal models for their potential to prevent huntingtin aggregation. Furthermore, they can be evaluated for their therapeutic potential in HD animal models by nanoparticle‐based delivery approaches to brain . They can also be utilized as chemical tools for deciphering aggregation pathways in animal and cell models of HD.…”

“…Furthermore, they can be evaluated for their therapeutic potential in HD animal models by nanoparticle-based delivery approaches to brain. [80,81] They can also be utilized as chemical tools for deciphering aggregation pathways in animal and cell models of HD.…”

Preparation and Drug Release Study of Novel Nanopharmaceuticals with Polysorbate 80 Surface Adsorption

“…With ΔH < 0 and ΔS < 0, the electrostatic force is the main force [41]. Therefore, according to our ITC findings, the hydrophobic interaction plays an [42], polysorbate 80 may have structural changes in the initial phase of the adsorption of CHP NPs, and the acyl chain obtains a highly flexible structure. At the time of adsorption, the acyl chain (−CH 2 ) and ester group (−C=O) interacting with the CHP NPs under the hydrophobic interaction force dominate hydrogen bonding, so the polysorbate 80 molecule approaches the surface of the CHP NPs to result in the monolayer plane structure (Figure 8(b)), which is consistent with the experimental results.…”

Analytical validation of an ATR-FTIR based method for quantifying the amount of polysorbate 80 adsorbed on PLGA nanoparticles

“…The extracted surfactant was then quantified using ATR-FTIR response and by plotting the values in the standard curve. (14) However, in the drug development to clinical translation efforts, an analytical method or a process needs to be validated for their usage in manufacturing and quality control. International Regulatory agencies such as Food and Drug Administration (FDA) encourages analytical method validation related to the production of any new drug form or for the application of Investigational New Drug (IND).…”