Analytical validation of an ATR-FTIR based method for quantifying the amount of polysorbate 80 adsorbed on PLGA nanoparticles

Abstract

Nanoparticle-based drug delivery systems for crossing blood-brain-barrier employ diverse strategies. Coating of the nanoparticles with non-ionic surfactants is often employed for enhancing the delivery process. Polysorbate 80 is one of...

Non-Ionic Surfactants for Stabilization of Polymeric Nanoparticles for Biomedical Uses

“…Therefore, the development of analytical methods that quantify the amount of PS present on the surface of the NP takes relevance. These methods aim to monitor and solve the optimum amount of PS [61,[90][91][92], avoiding undesirable reactions.…”

Stabilization of Carbon Nanotubes and Graphene by Tween-80: Mechanistic Insights from Spectroscopic and Simulation Studies

“…The FTIR assignments are based on those reported elsewhere . FTIR of pure Tween-80 exhibited peaks characteristic of Tween-80 such as those corresponding to ethylene oxide residues (C–O vibrations at 1092 cm –1 ), methylene groups of acyl/fatty acid chain (C–H scissoring vibrations at 1459 cm –1 , symmetric stretching vibrations at 2857 cm –1 , asymmetric stretching vibrations at 2922 cm –1 , CC vibrations at 1641 cm –1 ), and ester bond (stretching vibrations of CO bond at 1735 cm –1 ) as well as O–H stretching vibrations at 3460 cm –1 due to water/moisture. , The FTIR spectrum of 10 mg/mL of Tween-80 solution overlapped with the spectrum of pure Tween-80 with minor peak shifts as noted below. The EO peak (1095 cm –1 ) and ester peak (1736 cm –1 ) shifted to a slightly higher wavenumber in 10 mg/mL of Tween-80.…”

“…These results indicate a decrease in the fluidity of the methylene groups of the fatty acid chain, suggesting that Tween-80 interacts with SWCNTs via the fatty acid chain. 40,41 Similarly, interactions between MWCNT/graphene and Tween-80 were also studied at 0.5−1 mg CNP/mL. Major shifts were observed at the peak corresponding to asymmetric stretching of methylene in the case of MWCNTs, while the peak corresponding to C�C was shifted in both MWCNT and graphene (Figure 4).…”

Discrimination of commercial ibuprofen tablets by using a button sample holder and mid-infrared spectroscopy

“…27–29 The shoulder at 3560 cm −1 may indicate the presence of polysorbate 80. 30–32 The E-IBP coating spectrum contains a sharp band near 1700 cm −1 characteristic of the CO stretching vibration of ibuprofen that is not present in the A-IBP spectrum. 33–35 This band may be due to incorporation of ibuprofen in the coating, or because a thinner coating was used to make the E-IBP tablets, resulting in part of the interior being sampled when the coating surface was removed for analysis.…”