Deciphering the Factors for Nodulation and Symbiosis of Mesorhizobium Associated with Cicer arietinum in Northwest India

Singh, Raghvendra Pratap; Manchanda, Geetanjali; Yang, Yingjie; Singh, Devraj; Srivastava, Alok Kumar; Dubey, Ramesh Chandra; Zhang, Chengsheng

doi:10.3390/su11247216

Cited by 20 publications

(12 citation statements)

References 69 publications

(76 reference statements)

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“…Bacterial strains showing catalase activity must be highly resistant to environmental, mechanical and abiotic stress [31]. Plant growth promotion properties, resistance to environmental stress support the rhizobia in their saprophytic survival (free-living state), effective nodulation with legumes, and consequently their symbiotic efficiency as stated by Singh et al [32].…”

Section: Discussionmentioning

confidence: 94%

Characterization of Indigenous Rhizobia Strains Associated to Soybean [Glycine max (L.) Merrill] in Benin

Zoundji¹,

Houngnandan²,

Boko

et al. 2020

IJPSS

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Legumes such as soybean establish symbiotic relation with nitrogen fixing bacteria such as Rhizobia. Nitrogen fixation via legume-rhizobium symbiosis is the most important source of Nitrogen in agro-ecosystems. But environmental stresses are important limiting factors for this process. Hence, the aim of this study is to evaluate the physiological characteristics and Plant Growth Promoting (PGP) properties of soybean rhizobia. A total of 28 Rhizobia strains obtained from soybean root nodules collected in from three Agro-Ecological Zones (zones 3, 4 and 5) producers of soybean in Benin were used. The physiological characteristics include utilization of carbon source, tolerance to temperature, salinity and pH, resistance to antibiotics and heavy metals. The PGP properties were relative to production of indole, hydrogen cyanide and ammonia and catalase test. The results revealed that, irrespective of their geographical regions, the 28 isolates were grouped into five Clusters. Most of them tolerated neutral to alkaline pH and high salt stress and 17% of them could grow at 40°C. Most of them showed resistance to heavy metal and antibiotics. These isolates tested were able to use a broad range of carbohydrates as sole source of carbon. Production of indole, hydrogen cyanide and ammoniac were respectively found on 56%, 41% and 44% of isolates but all isolates gave positive reaction to catalase test. These rhizobial isolates showing best physiological and PGP properties could be good candidates to establish a successful symbiosis with soybean under the variation of environmental conditions that prevail in Benin.

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Section: Discussionmentioning

confidence: 94%

Characterization of Indigenous Rhizobia Strains Associated to Soybean [Glycine max (L.) Merrill] in Benin

Zoundji¹,

Houngnandan²,

Boko

et al. 2020

IJPSS

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“…Isolated strains were identified according to Singh et al (2019) . Briefly, genomic DNA was isolated using a Wizard® Genomic DNA Purification Kit (Promega) and 16S rRNA was amplified by PCR using 27f (5'-AGAGTTTGATCMTGGCTCAG-3') and 1492r (5'-GGTT-ACCTTGTACGACTT-3') universal primers with an Applied Biosystems PCR system.…”

Section: Methodsmentioning

confidence: 99%

“…The ability to fix nitrogen is widely, but sporadically, distributed among archaea and bacteria, including the families Proteobacteria, Firmicutes, Cyanobacteria, Actinobacteria, and Chlorobi ( Dos Santos et al, 2012 ). NifH is used as a marker gene to detect nitrogen-fixing microorganisms in the environment ( Igai et al, 2016 ; Fan et al, 2019 ), and nitrogen-fixing Rhizobiales have been identified in the special root nodules of crop legumes, such as alfalfa, beans, peas, and soy, which provide 20% of food protein worldwide ( Mnasri et al, 2012 ; Egamberdieva et al, 2013 ; de Almeida Lopes et al, 2016 ; Sharma et al, 2016 ; Singh et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Nodule and Root Zone Microbiota of Salt-Tolerant Wild Soybean in Coastal Sand and Saline-Alkali Soil

et al. 2020

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Soil salinization limits crop growth and yield in agro-ecosystems worldwide by reducing soil health and altering the structure of microbial communities. Salt-tolerant plant growthpromoting rhizobacteria (PGPR) alleviate plant salinity stress. Wild soybean (Glycine soja Sieb. and Zucc.) is unique in agricultural ecosystems owing to its ability to grow in salinealkali soils and fix atmospheric nitrogen via symbiotic interactions with diverse soil microbes. However, this rhizosphere microbiome and the nodule endosymbionts have not been investigated to identify PGPR. In this study, we investigated the structural and functional rhizosphere microbial communities in saline-alkali soil from the Yellow River Delta and coastal soil in China, as well as wild soybean root nodule endosymbionts. To reveal the composition of the microbial ecosystem, we performed 16S rRNA and nifH gene amplicon sequencing on root nodules and root zones under different environmental conditions. In addition, we used culture-independent methods to examine the root bacterial microbiome of wild soybean. For functional characterization of individual members of the microbiome and their impact on plant growth, we inoculated isolates from the root microbiome with wild soybean and observed nodulation. Sinorhizobium/Ensifer accounted for 97% of the root nodule microbiome, with other enriched members belonging to the phyla Actinobacteria, Bacteroidetes, Chloroflexi, Acidobacteria, and Gemmatimonadetes; the genera Sphingomonas, Microbacterium, Arthrobacter, Nocardioides, Streptomyces, Flavobacterium, Flavisolibacter, and Pseudomonas; and the family Enterobacteriaceae. Compared to saline-alkali soil from the Yellow River Delta, coastal soil was highly enriched for soybean nodules and displayed significant differences in the abundance and diversity of β-proteobacteria, δ-proteobacteria, Actinobacteria, and Bacteroidetes. Overall, the wild soybean root nodule microbiome was dominated by nutrient-providing Sinorhizobium/ Ensifer and was enriched for bacterial genera that may provide salt resistance. Thus, this reductionist experimental approach provides an avenue for future systematic and functional studies of the plant root microbiome.

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“…Chickpea is a rich source of dietary protein, providing as much as 17-23% protein as compared with cereals which provide only about 8-10%. Chickpea plays a significant role in improving soil fertility by fixing the atmospheric nitrogen; it can fi x up to 60kgN -ha per year [8]. The plant growth promotion and symbiotic performances of the isolates revealed the causes and highlighted the factors for effi cient nodulation in chickpea [8].…”

Section: Background and Justifi Cationmentioning

confidence: 99%

“…Chickpea plays a significant role in improving soil fertility by fixing the atmospheric nitrogen; it can fi x up to 60kgN -ha per year [8]. The plant growth promotion and symbiotic performances of the isolates revealed the causes and highlighted the factors for effi cient nodulation in chickpea [8]. Chickpea has high potential for domestic and export market.…”

Section: Background and Justifi Cationmentioning

confidence: 99%

Effect of integrating chickpea varieties with insecticides for the management of pod borer (Helicoverpa armigera Hubner) (Lepidoptera: Noctudae)

Dagne¹,

Teshome²

2021

J Agric Sc Food Technol

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Chickpea (Cicer arietinum L.) is the world's second most important grain legume after common bean (Phaseolus vulgaris L.). Ethiopia is considered as a secondary center of genetic diversity for chickpea. The fi eld experiment was conducted at Ginir district with the objectives to evaluate the effective management option against chickpea pod borer and to determine the optimum frequencies of the insecticide for the management of chickpea pod borer. The experiment was conducted using two chick pea varieties; Habru (more preferred) and Arerit (less preferred) and two insecticide Highway 50% EC (400ml/ha) and Nimbicidine (3lt/ha). The result revealed that both insecticides are effective against pod borer even if they have slight percent larval reductions. The pod borer damage reduction ranged from 56.83% to 69.94% and on Highway 50% EC treated plots as compared to the control on Habru variety. On the variety Arerit up to 76.30% larval reduction on the Highway 50% EC treated plot was occurred as compared to control. The minimum 36.17% larval reduction was occurred on the plot treated with Nimbicidine on the variety Habru. The Maximum percent of seed yield (57.95% and 57.95%) increased over check was occurred by Highway 50% EC one and two times treaded plots on the variety Arerit, respectively. The plot sprayed one and two times with Highway 50%EC gave the maximum net return ETB 178,959.8ha-1 and ETB 178,402ha-1 on the variety Arerti and the unsprayed plot of the variety Arerti gave the minimum net returns ETB 15,054ha-1. It is recommended that these insecticides with specially Arerit variety are suggested to the growers for management of the pod borer population below economic threshold level under fi eld conditions.

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Deciphering the Factors for Nodulation and Symbiosis of Mesorhizobium Associated with Cicer arietinum in Northwest India

Cited by 20 publications

References 69 publications

Characterization of Indigenous Rhizobia Strains Associated to Soybean [Glycine max (L.) Merrill] in Benin

Characterization of Indigenous Rhizobia Strains Associated to Soybean [Glycine max (L.) Merrill] in Benin

Nodule and Root Zone Microbiota of Salt-Tolerant Wild Soybean in Coastal Sand and Saline-Alkali Soil

Effect of integrating chickpea varieties with insecticides for the management of pod borer (Helicoverpa armigera Hubner) (Lepidoptera: Noctudae)

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