Decellularized Extracellular Matrix Containing Electrospun Fibers for Nerve Regeneration: A Comparison Between Core–Shell Structured and Preblended Composites

Deng, Rongli; Luo, Ziling; Rao, Zilong; Lin, Zhuogeng; Chen, Shihao; Zhou, Jing; Zhu, Qian; Liu, Xiaolin; Bai, Ying

doi:10.1007/s42765-021-00124-5

Cited by 55 publications

(31 citation statements)

References 59 publications

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“…[ 42 ] Similar behavior of the cells was seen on the dBECM‐coated PBAT fibers, in addition, the cells on dBECM‐coated matrices produced longer filopodia as well as they formed slight blebbings (Figure 4D). [ 43 ]…”

Section: Resultsmentioning

confidence: 99%

“…[42] Similar behavior of the cells was seen on the dBECMcoated PBAT fibers, in addition, the cells on dBECM-coated matrices produced longer filopodia as well as they formed slight blebbings (Figure 4D). [43] To confirm the biocompatibility of random and aligned PBAT membranes w/wo dBECM modification, we conducted MTS testing. In all conditions, hFOB cells showed a good proliferating ability from day 3 to 7, confirming the cytocompatibility of the components (Figure 4E).…”

Section: Physical and Biological Assessment Of Dbecm-coated Electrosp...mentioning

confidence: 99%

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Decellularized Bone Extracellular Matrix‐Coated Electrospun PBAT Microfibrous Membranes with Cell Instructive Ability and Improved Bone Tissue Forming Capacity

Karakaya

Erdoğan

Arslan

et al. 2022

Macromolecular Bioscience

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Current approaches to develop bone tissue engineering scaffolds have some limitations and shortcomings. They mainly suffer from combining mechanical stability and bioactivity on the same platform. Synthetic polymers are able to produce mechanically stable sturctures with fibrous morphology when they are electrospun, however, they cannot exhibit bioactivity, which is crucial for tissue engineering and regenerative medicine. One current strategy to bring bioactivity in synthetic materials is to combine extracellular matrix (ECM)‐sourced materials with biologically inert synthetic materials. ECM‐sourced materials without any modifications are mechanically unstable; therefore, reinforcing them with mechanically stable platforms is indispensable. In order to overcome this bifacial problem, we have demonstrated that poly(butylene adipate‐co‐terephthalate) (PBAT) electrospun microfibrous membranes can be successfully modified with decellularized bone ECM to endow fibers with bioactive hydrogel and mimic natural micro‐features of the native bone tissue. The developed structures have been shown to support osteogenesis, confirmed by histochemical staining and gene expression studies. Furthermore, ECM‐coated PBAT fibers, when they were aligned, supplied an improved level of osteogenesis. The strategy demonstrated can be adapted to any other tissues, and the emerging microfibrous, mechanically stable, and bioactive materials can find implications in the specific fields of tissue engineering and regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

Section: Physical and Biological Assessment Of Dbecm-coated Electrosp...mentioning

confidence: 99%

Decellularized Bone Extracellular Matrix‐Coated Electrospun PBAT Microfibrous Membranes with Cell Instructive Ability and Improved Bone Tissue Forming Capacity

Karakaya

Erdoğan

Arslan

et al. 2022

Macromolecular Bioscience

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“…Liu et al ( 2021 ) prepared a porcine decellularized peripheral nerve matrix hydrogel, which exhibited high bioactivity in promoting axonal extension, SCs alignment, and nerve fiber remyelination. A consecutive study showed that the decellularized nerve matrix hydrogel coated electrospun PLLA nanofibers significantly promoted SCs migration and axonal extension (Chen et al, 2019 ; Deng et al, 2022 ). The synergistic effects of the decellularized nerve matrix hydrogel and the aligned nanofibers were further confirmed in vivo regarding nerve fiber fasciculation, myelination, and functionalization (Zheng et al, 2021 ).…”

Section: Schwann Cell-derived Biomaterialsmentioning

confidence: 99%

Biomaterial-Based Schwann Cell Transplantation and Schwann Cell-Derived Biomaterials for Nerve Regeneration

Rao

Lin

Song

et al. 2022

Front. Cell. Neurosci.

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Schwann cells (SCs) dominate the regenerative behaviors after peripheral nerve injury by supporting axonal regrowth and remyelination. Previous reports also demonstrated that the existence of SCs is beneficial for nerve regeneration after traumatic injuries in central nervous system. Therefore, the transplantation of SCs/SC-like cells serves as a feasible cell therapy to reconstruct the microenvironment and promote nerve functional recovery for both peripheral and central nerve injury repair. However, direct cell transplantation often leads to low efficacy, due to injection induced cell damage and rapid loss in the circulatory system. In recent years, biomaterials have received great attention as functional carriers for effective cell transplantation. To better mimic the extracellular matrix (ECM), many biodegradable materials have been engineered with compositional and/or topological cues to maintain the biological properties of the SCs/SCs-like cells. In addition, ECM components or factors secreted by SCs also actively contribute to nerve regeneration. Such cell-free transplantation approaches may provide great promise in clinical translation. In this review, we first present the current bio-scaffolds engineered for SC transplantation and their achievement in animal models and clinical applications. To this end, we focus on the physical and biological properties of different biomaterials and highlight how these properties affect the biological behaviors of the SCs/SC-like cells. Second, the SC-derived biomaterials are also reviewed and discussed. Finally, the relationship between SCs and functional biomaterials is summarized, and the trends of their future development are predicted toward clinical applications.

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“…8,9 Creative strategies are needed to promote axon regrowth for large-scale nerve defect treatment. Some novel biomaterials have been reported to replace ANGs and improve the PNI treatment, such as decellularized allografts, 10,11 artificial polymers and scaffolds, 12,13 and natural materials. 14 However, some drawbacks are found for these biomaterials for the repair of damaged nerves, such as immunological rejection, immune response, poor biodegradability, high complexity of the synthesis process, and more importantly, an unsatisfactory axon-promoting effect.…”

Section: Introductionmentioning

confidence: 99%

Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation

et al. 2023

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Decellularized Extracellular Matrix Containing Electrospun Fibers for Nerve Regeneration: A Comparison Between Core–Shell Structured and Preblended Composites

Cited by 55 publications

References 59 publications

Decellularized Bone Extracellular Matrix‐Coated Electrospun PBAT Microfibrous Membranes with Cell Instructive Ability and Improved Bone Tissue Forming Capacity

Decellularized Bone Extracellular Matrix‐Coated Electrospun PBAT Microfibrous Membranes with Cell Instructive Ability and Improved Bone Tissue Forming Capacity

Biomaterial-Based Schwann Cell Transplantation and Schwann Cell-Derived Biomaterials for Nerve Regeneration

Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation

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