Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation

Abstract

RGD-displaying phage, fibrin, and neural stem cells (NSCs) are integrated to form a fiber-like hydrogel, which promotes preferential differentiation of NSCs and serves as a nerve graft to repair the long-gap nerve defect by enhanced neurogenesis.

Harnessing filamentous phages for enhanced stroke recovery

“…Filamentous phage regenerative therapy presents a promising pathway for enhancing stroke recovery, unveiling novel mechanisms for safeguarding and restoring post-stroke brain tissue. Bacteriophages showcase varied capabilities, spanning pathogen clearance, immune modulation, promotion of neurogenesis, and enhancement of vascular function ( 22 – 24 ). These combined effects culminate in neuroprotection, diminished edema, reduction in infarct size, and improved neural functionality.…”

“…The modified filamentous bacteriophage promotes the generation of oligodendrocyte precursor cells, stimulates myelin regeneration, significantly enhances neural function and cognitive abilities, and facilitates neural repair. Additionally, it serves as a potential alternative to autologous nerve transplants for restoring extensive nerve damage ( 22 , 26 ). These investigations not only underscore the promising potential of phage therapy in the context of enhancing stroke recovery but also emphasize the need for meticulous parameter optimization encompassing aspects like selection, dosing, administration routes, and temporal considerations to ensure heightened safety and efficacy.…”

Gelatin-Based Injectable Hydrogels Loaded with Copper Ion Cross-linked Tannic Acid Nanoparticles for Irregular Wound Closure Repair

“…Many materials have been developed to prepare injectable hydrogel wound dressings, such as natural polymers (gelatin, chitosan, , alginate, , fibrin, , etc.) and synthetic polymers (poly­(vinyl alcohol), , poly­(acrylic acid), etc.).…”

Plasma fibrin membranes loaded with bone marrow mesenchymal stem cells and corneal epithelial cells promote corneal injury healing via attenuating inflammation and fibrosis after corneal burns

“…32 Its inherent advantages include the ability to obtain the raw material of PFMs from the patient's own blood, without the risk of rejection, and its natural biocompatibility. 33 The network structure formed by fibrin cross-linking can facilitate cell attachment and material exchange. Compared with the new material electro-spun poly(lactic-co-glycolic acid) (PLGA) membrane, its cell adhesion ability may not be as good as that of the electro-spun PLGA membrane, but the raw material of the plasma fibrin membrane is biomaterial plasma, which contains some growth factors, which can promote the repair of tissue defects.…”