Decellularization of rat aortic valve allografts reduces leaflet destruction and extracellular matrix remodeling

Abstract

Damage to the valves caused by decellularization technique is much less than the damage caused by the recipient's immune response. In vitro removal of viable cells in (cryopreserved) homografts may decrease graft failure. Seeding with autologous or major histocompatibility complex-matched donor endothelial cells will be necessary to diminish damage induced by an absent blood-tissue barrier.

The influence of heart valve leaflet matrix characteristics on the interaction between human mesenchymal stem cells and decellularized scaffolds

“…2G). On the other hand, it may be that detergent procedures used for heart valve decellularization could affect the antigenic stability of the collagen-elastic matrix (or induce cryptic epitopes) in scaffolds from xeno-and (to a lesser extent) allogeneic donors [36][37][38]. Ongoing studies at our laboratory on immunocompetent and immunodeficient rats transplanted with intact or decellularized porcine leaflets Fig.…”

Preparation of Ex Vivo–Based Biomaterials Using Convective Flow Decellularization

“…9,15,[23][24][25][26][27][28] Human saphenous veins have been decellularized by placing the specimens in a 378C sodium dodecyl sulfate (SDS) solution …”

“…29 Following similar technologies, others have produced acellular liver stroma, 8 rat aortic valve, 24,26 and iliac artery. 30 Although the molecular details are not clear, some methods are more effective than others.…”

Pericardial Processing: Challenges, Outcomes and Future Prospects

“…Chemical protocols involve use of alkaline and acid treatments (Freytes et al, 2004), ionic detergents, sodium dodecyl sulfate (SDS), sodium deoxycholate and Triton X-200 (Rieder et www.intechopen.com al. 2004;Hudson et al, 2004), non-ionic detergents, such as Triton X-100 (Grauss et al, 2003), zwitterionic detergents (Dahl et al, 2003), tri(n-butyl)phosphate (Woods & Gratzer, 2005) as well as hypertonic or hypotonic solutions (Goissis et al, 2000;Woods & Gratzer, 2005;Vyavahareet al, 1997;Dahl et al, 2003). These modalities will either mediate lysis of the cells or solubilization of the cellular components.…”