The influence of heart valve leaflet matrix characteristics on the interaction between human mesenchymal stem cells and decellularized scaffolds

Iop, Laura; Renier, Vera; Naso, Filippo; Piccoli, Martina; Bonetti, Antonella; Gandaglia, Alessandro; Pozzobon, Michela; Paolin, Adolfo; Ortolani, Fulvia; Marchini, Maurizio; Spina, Michele; Coppi, Paolo De; Sartore, Saverio; Gerosa, Gino

doi:10.1016/j.biomaterials.2009.04.031

Cited by 77 publications

(107 citation statements)

References 36 publications

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“…Recently, new methodologies of decellularization have been experimented in tissue engineering for improving the in vivo performance of HVs. 39 Mimicking the three-dimensional structure and nanotopography of native tissues, the porcine valve conduits prepared accordingly to the TriCol protocol 16,25,26 demonstrated in preclinical models a great chance of success in 1) promoting a spontaneous guided tissue regeneration 39 and 2) overcoming the typical drawbacks of valve graft transplantation, that is, high risk of calcification, fibrosis, and immunological reaction. 19 The successful cell integration and differentiation into tissuespecific phenotypes are demonstrated to be driven by the optimized hemodynamic profile, mechanical stress, and matrikine signaling of HVs.…”

Section: Discussionmentioning

confidence: 99%

“…Combining Triton X-100 and cholate, the so-called TriCol procedure, has been reported to guarantee 1) the complete tissue decellularization, that is functional to control the development of calcification process; 24 2) the removal of alpha-gal xenoantigens for avoiding the rejection of xenogenic grafts; 25 3) the preservation of valve hemodynamic profile and performance; and 4) in vitro full biocompatibility with valve interstitial cells 26 and bone marrow mesenchymal stem cells (BM-MSCs). 16 Largely characterized by their composition of glycosaminoglycans, proteoglycans, ECM filaments, 24,27 and nonpreconditioned TriCol heart valves showed a good hemodynamic profile and stable structural integrity in a long-term follow-up performed in allogeneic animal model. 18 There is a general agreement that bone marrow stromal cells are recruited into circulation upon tissue damage or graft implantation and are involved in tissue repair and regeneration.…”

Section: Introductionmentioning

confidence: 95%

“…4,15 In contrast, sodium cholate-and deoxycholate-based methods are useful to achieve fully nude matrices, with high in vitro and/or in vivo repopulation potentialities. [16][17][18][19] Recently, the development of decellularized xenografts or homografts with self-regeneration potential is considered as an attractive option to obtain novel viable replacements granting longer durability and avoiding the extensive in vitro cell conditioning. Various studies have already reported in situ recellularization of decellularized xenografts and homografts in animal models highlighting variable success rate in dependence on the adopted decellularization method or used graft typology (xenograft or homograft).…”

Section: Introductionmentioning

confidence: 99%

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Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells

Liddo¹,

Andrea²,

Barbon³

et al. 2016

IJN

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Considerable progress has been made in recent years toward elucidating the correlation among nanoscale topography, mechanical properties, and biological behavior of cardiac valve substitutes. Porcine TriCol scaffolds are promising valve tissue engineering matrices with demonstrated self-repopulation potentiality. In order to define an in vitro model for investigating the influence of extracellular matrix signaling on the growth pattern of colonizing blood-derived cells, we cultured circulating multipotent cells (CMC) on acellular aortic (AVL) and pulmonary (PVL) valve conduits prepared with TriCol method and under no-flow condition. Isolated by our group from Vietnamese pigs before heart valve prosthetic implantation, porcine CMC revealed high proliferative abilities, three-lineage differentiative potential, and distinct hematopoietic/endothelial and mesenchymal properties. Their interaction with valve extracellular matrix nanostructures boosted differential messenger RNA expression pattern and morphologic features on AVL compared to PVL, while promoting on both matrices the commitment to valvular and endothelial cell-like phenotypes. Based on their origin from peripheral blood, porcine CMC are hypothesized in vivo to exert a pivotal role to homeostatically replenish valve cells and contribute to hetero- or allograft colonization. Furthermore, due to their high responsivity to extracellular matrix nanostructure signaling, porcine CMC could be useful for a preliminary evaluation of heart valve prosthetic functionality

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells

Liddo¹,

Andrea²,

Barbon³

et al. 2016

IJN

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“…Acellularization process can also affect the efficiency of cell culture on the scaffold. In the study of Iop [21] human and porcine heart valves were acellularized, then HBM-MSC (Human Bone Morrow-Mesenchymal Stem Cells) seeded statically on the ventricularis fibrosa layers and grown for 30 days. It was observed that the characteristics of the cell growth on both layers (fibrosa and ventricularis) was different suggesting that cell growth may be determined by changes in the microstructure of the ECM.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility Issue Of Tissue Engineered Heart Valves

Wilczek¹

2015

Archives of Metallurgy and Materials

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Tissue engineering is a new field of knowledge which creates the possibilities for producing bioactive cardiac prostheses that will characterize by biomechanical and morphological properties similar to native tissue. It is expected that it will be characterized by high durability, which is very important from the social and clinical point of view. The aim of the study was to compare the cytotoxic effect of enzymatic and detergent acellularization methods commonly used for the biological scaffold preparation. It seems that the use of enzymatic methods, allows efficient donor cells removal while maintaining the ability to autologous cell seeding. Heart valves bioprosthesis created using these techniques, may be a good alternative to the currently used prostheses.Keywords: tissue engineering, heart valve, cytotoxicity, scaffold.Inżynieria tkankowa jest nową dziedziną wiedzy, która stwarza możliwości wytwarzania protez serca bioaktywnych charakteryzujących się właściwościami biomechanicznymi i morfologicznymi zbliżonymi do tkanki rodzimej. Oczekuje się, że proteza serca będzie charakteryzować się wysoką trwałością, co jest bardzo ważne z punktu widzenia społecznego i klinicznego. Celem badania było porównanie efektu cytotoksycznego enzymatycznych i detergentowych metod usuwania komórek, powszechnie stosowanych do wytwarzania biologicznych rusztowań. Wydaje się, że stosowanie metod enzymatycznych, umożliwia wydajne usunięcie komórek dawcy przy zachowaniu zdolności do autologicznego posiewu komórek. Bioprotezy zastawek serca tworzone za pomocą tej techniki mogą być dobrą alternatywą dla obecnie stosowanych mechanicznych i biologicznych protez zastawek serca.

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“…In fact, it provides a suitable preservation of the texture of native valve interstitium and hemodynamic performance [15][16][17], with concurrent removal of the xenoantigen galactose-α-1,3-galactose (alpha-gal) [18]. In addition, TRICOL-treated porcine aortic valve conduits (AVCs) were shown to exhibit physiological performance for up to 15 months after implantation in Vietnamese Pigs (VPs) as well as permissivity to repopulation by recipient's cells and tissue remodeling capability, as revealed by ex vivo morphological analyses [19,20].…”

Section: Introductionmentioning

confidence: 99%

Decellularized aortic conduits: could their cryopreservation affect post-implantation outcomes? A morpho-functional study on porcine homografts

et al. 2016

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Explanted D grafts exhibited near-normal features, whereas the presence of calcification, inflammatory infiltrates, and disarray of elastic lamellae occurred in some DC grafts. In the unaltered regions of AVCs from both groups, almost complete re-endothelialization was observed for both valve cusps and aorta walls. In addition, side-by-side repopulation by recipient's fibroblasts, myofibroblasts, and smooth muscle cells was paralleled by ongoing tissue remodeling, as revealed by the ultrastructural identification of typical canals of collagen fibrillogenesis and elastogenesis-related features. Incipient neo-vascularization and re-innervation of medial and adventitial tunicae of grafted aortic walls were also detected for both D and DC groups. Cryopreservation did not affect post-implantation AVC hemodynamic behavior and was topically propensive to cell repopulation and tissue renewal, although graft deterioration including calcification was present in several areas. Thus, these preliminary data provide essential information on feasibility of decellularization and cryopreservation coupling in the perspective of treatment optimization and subsequent clinical Abstract Decellularized porcine aortic valve conduits (AVCs) implanted in a Vietnamese Pig (VP) experimental animal model were matched against decellularized and then cryopreserved AVCs to assess the effect of cryopreservation on graft hemodynamic performance and propensity to in vivo repopulation by host's cells. VPs (n = 12) underwent right ventricular outflow tract substitution using AVC allografts and were studied for 15-month follow-up. VPs were randomized into two groups, receiving AVCs treated with decellularization alone (D; n = 6) or decellularization/ cryopreservation (DC; n = 6), respectively. Serial echocardiography was carried out to follow up hemodynamic function. All explanted AVCs were processed for light and electron microscopy. No signs of dilatation, progressive stenosis, regurgitation, and macroscopic calcification were echocardiographically observed in both D and DC groups. Heart Vessels 1 3 trials using similarly treated human allografts as innovative heart valve substitutes.

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The influence of heart valve leaflet matrix characteristics on the interaction between human mesenchymal stem cells and decellularized scaffolds

Cited by 77 publications

References 36 publications

Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells

Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells

Biocompatibility Issue Of Tissue Engineered Heart Valves

Decellularized aortic conduits: could their cryopreservation affect post-implantation outcomes? A morpho-functional study on porcine homografts

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