Data to the sex determination in Pistacia species using molecular markers

Abstract

Sex identification in Pistacia species during the long juvenile stage is an economically desirable objective. Due to the lack of morphological methods to identify sex at this stage, the application of molecular markers is expected to facilitate breeding programs. The aim of our study was to identify a marker closely linked to sex loci in Pistacia atlantica Desf subsp. mutica, P. khinjuk, and P. vera subsp. Sarakhs. Samples were collected from both male and female plants of each species, and their band patterns were analyzed according to the presence or absence of specific bands. Thirty random amplified polymorphic DNA (RAPD) primers and a pair of sequence characterized amplified region (SCAR) primers were tested as potential markers of sex in wild Pistacia species. Among the RAPD primers, only BC 1200 was found to amplify a specific sex band present in female plants. Based on our analysis of all individual samples, a fragment of approximately 300 bp was amplified in female trees but absent in male ones. Although sex determination mechanisms in Pistacia are still unknown, they may be controlled by a single locus that acts as a trigger. The SCAR technique has proved to be a reliable technique in gender determination of pistachio genotypes at the seedling phenophase. This method could reduce both the time and costs associated with breeding programs.

Proteomic Identification of Differentially Expressed Proteins between Male and Female Plants in Pistacia chinensis

“…These techniques need to design and try different sets of random primers [4]–[7] and the function of amplified products is often unclear [5], [6], while proteomic analysis directly identify the changes of gene products (proteins) in abundance [10] and in most cases the functions of these proteins identified are known. As for sex determination, both techniques are appropriate if they can distinguish male and female plants.…”

“…For this purpose, a range of sex-linked molecular markers such as RAPD, RFLP, AFLP and microsatellite have been generated [3]. In Pistacia species, male- or female-specific RAPD markers have been identified [4]–[6]. In S. latifolia , a gene encoding a male-specific protein is linked to the X chromosome [1].…”

Risk assessment of exposure to aflatoxin B1 and ochratoxin A through consumption of different Pistachio ( Pistacia vera L.) cultivars collected from four geographical regions of Iran

“…Remarkably, native Pistacia vera var. sarakhs is used as rootstock (Esfandiyari et al, 2012 ▶). Pistachio kernel is known as a rich source of natural antioxidants, sterols, vitamins, minerals, fatty acids and phenolic compounds.…”

Sex-oriented research on dioecious crops of Indian subcontinent: an updated review

“…mutica, P. khinjuk, and P. vera subsp. sarakhs) using thirty different RAPD primers and out of them only BC1200 showed a female-specific amplification at around 1200 bp (Esfandiyari et al 2011). Additionally, an SCAR marker was identified in that study which was able to amplify a 300 bp region from the female plant only.…”