Abstract:The appearance of activated lymphocytes and antibodies in the circulation of rats was monitored following allogeneic kidney, skin, or combined skin-spleen transplants. Lymphocyte activation was measured by 3H-thymidine incorporation, and antibody by a hemolytic assay. These assays have been developed so that small samples of blood could be used, thus maintaining recipient survival and allowing for daily correlation between the response observed and the fate of the graft. These responses were detected after all… Show more
“…All three untreated allograft recipients ( C , D, E) showed both proliferative and antibody responses. As previously shown in the Lewis strain (Smart et al 1978) the peak of the proliferative response as well as the rate of antibody formation was more variable than is seen in DA recipients.…”
Section: Passive Enhancement In the (Da X Lewis) Fl Into Lewis Modelsupporting
confidence: 65%
“…Studies on allogeneic and isogeneic renal transplant recipients in the (DA x Lewis) F1 into DA and Lewis combinations have indicated that the proliferative response of peripheral lymphocytes is specific, that it invariably precedes rejection, and that its magnitude may be related to the magnitide of subsequent rejection (Smart et al 1978). In 10 of the 11 recipients which have been treated with enhancing antiserum in this study the proliferative response of peripheral lymphocytes and the hemolytic antibody response have been depressed.…”
Section: Disczkssionmentioning
confidence: 58%
“…The renal and skin transplantation techniques have been described in a previous 30 1 T paper (Smart et al 1978). DA anti-Lewis and Lewis anti-DA enchancing sera were prepared as described by Fabre & Morris (1973), using one-tenth spleen equivalents for immunization.…”
Section: Transplatation and Passive Enhancementmentioning
confidence: 99%
“…The percentage cell death was measured following the addition of 4~1 of rabbit complement. These techniques have been described in detail in a previous paper (Smart et al 1978).…”
Section: Measztrement Of Lymphocyte Proliferation and Hemolytic Antibodymentioning
Specific suppression of renal allograft rejection in two strain combinations of rats by passive enhancement was associated with depression of both peripheral lymphocyte proliferation and hemolytic antibody production. Variations in these responses were related to allograft survival in individual rats. In general, these studies confirm the value of monitoring both the lymphocyte proliferation and hemolytic antibody responses for the prediction of subsequent rejection, and for evaluating the efficacy of the enhancement therapy.
“…All three untreated allograft recipients ( C , D, E) showed both proliferative and antibody responses. As previously shown in the Lewis strain (Smart et al 1978) the peak of the proliferative response as well as the rate of antibody formation was more variable than is seen in DA recipients.…”
Section: Passive Enhancement In the (Da X Lewis) Fl Into Lewis Modelsupporting
confidence: 65%
“…Studies on allogeneic and isogeneic renal transplant recipients in the (DA x Lewis) F1 into DA and Lewis combinations have indicated that the proliferative response of peripheral lymphocytes is specific, that it invariably precedes rejection, and that its magnitude may be related to the magnitide of subsequent rejection (Smart et al 1978). In 10 of the 11 recipients which have been treated with enhancing antiserum in this study the proliferative response of peripheral lymphocytes and the hemolytic antibody response have been depressed.…”
Section: Disczkssionmentioning
confidence: 58%
“…The renal and skin transplantation techniques have been described in a previous 30 1 T paper (Smart et al 1978). DA anti-Lewis and Lewis anti-DA enchancing sera were prepared as described by Fabre & Morris (1973), using one-tenth spleen equivalents for immunization.…”
Section: Transplatation and Passive Enhancementmentioning
confidence: 99%
“…The percentage cell death was measured following the addition of 4~1 of rabbit complement. These techniques have been described in detail in a previous paper (Smart et al 1978).…”
Section: Measztrement Of Lymphocyte Proliferation and Hemolytic Antibodymentioning
Specific suppression of renal allograft rejection in two strain combinations of rats by passive enhancement was associated with depression of both peripheral lymphocyte proliferation and hemolytic antibody production. Variations in these responses were related to allograft survival in individual rats. In general, these studies confirm the value of monitoring both the lymphocyte proliferation and hemolytic antibody responses for the prediction of subsequent rejection, and for evaluating the efficacy of the enhancement therapy.
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