The incidence of grass pollen, estimated by using a Burkard spore trap sited above a city building, is
correlated with meteorological factors. Pollen incidence was greatest on days with high maximum
temperatures and reduced on days of high humidity or rainfall. Winds from the north, north-west
and south-east carried the highest concentrations of pollen. Remote sensing image analysis has
been used to identify the grasslands over which these winds may have passed before entering the city.
An analysis of pollen yield and relative abundance of grasses in pastures and roadsides north of
Melbourne has implicated ryegrass (Lolium perenne and L. rigidum) and canary grass (Phalaris
tuberosa) as the major sources of atmospheric pollen.
The development of monoclonal mouse antibodies against ryegrass (Lolium perenne) pollen allergens is described. Hybridoma colonies secreting antibodies specific for allergenic components were detected using an enzyme-linked immunosorbent assay. Positive colonies were cloned and expanded. The pollen components with which the monoclonal antibodies interact were identified and characterised following sodium dodecyl sulphate polyacrylamide gel electrophoresis and electrophoretic transfer to nitrocellulose. In this paper six monoclonal mouse antibodies are described. Three antibodies interact with a single molecule of between 30,000 and 35,000 daltons. One antibody interacts with a component of 16,000 daltons whereas the remaining two antibodies react with more than one component, one reacting with two components at 28,000 and 30,000, and the other with five components having molecular weights between 18,000 and 71,000 daltons.
A series of four monoclonal antibodies has been prepared by hybridization of mouse myeloma cells with spleen cells from mice immunized with human blood granulocytes. The four antibodies all react specifically with granulocytes, failing to stain lymphocytes and other blood cells. Lymphocytic leukaemia cells are not stained, whereas myeloid leukaemia cells give a varied reaction with the antibodies. Studies on marrow, leukaemic cells and cell lines suggest that the four antibodies react with distinct differentiation antigens which are absent from myeloblasts, appear at the promyelocyte, myelocyte or metamyelocyte stage (depending on the antibody in question) and are expressed on all mature granulocytes.
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