DAF-fluorescence without NO: Elicitor treated tobacco cells produce fluorescing DAF-derivatives not related to DAF-2 triazol

Rümer, Stefan; Krischke, Markus; Fekete, Ágnes; Mueller, Martin J.; Kaiser, Werner M.

doi:10.1016/j.niox.2012.05.007

Cited by 36 publications

(25 citation statements)

References 25 publications

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“…NO induction by NUBS-4190 in potato is sharp and reproducible, and NUBS-4190 could be a good synthetic compound for NO induction in potato. Although there has been criticism about the specificity of DAFfluorescence for detecting NO (Rümer et al 2012), we confirmed NO generation by showing high expression of nitrate reductase genes StNR1 and StNR5 in NUBS-4190-treated potato (Fig. 6).…”

Section: Discussionsupporting

confidence: 65%

Defense induced by a bis-aryl methanone compound leads to resistance in potato against Phytophthora infestans

2013

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Plants recognize certain microbial compounds as elicitors of their active defense mechanisms. In the present study, NUBS-4190, a synthetic bis-aryl-methanone compound elicited NO and ROS generation in potato suspension cultured cells and intact potato leaves. Hypersensitive cell death was found in these cultured cells and in potato leaves without the accumulation of phytoalexins in the tubers. Defense-related genes such as StrbohB, StrbohC, StNR1, StNR5, Sthsr203J and StPR1 were expressed in potato suspension cultured cells treated with NUBS-4190. Resistance against Phytophthora infestans also increased in NUBS-4190-treated potato leaves.

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Section: Discussionsupporting

confidence: 65%

Defense induced by a bis-aryl methanone compound leads to resistance in potato against Phytophthora infestans

2013

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“…Since at least two distinct methods, based upon different chemical reactions, are recommended for the estimation of NO levels (Rümer et al 2012), NO status was investigated in citrus plants using electrochemical study, Griess reaction and CLMS analysis. Contents of nitrite and NOx along with DAF-2DA fluorescence were found to increase in leaves and roots exposed to PEG treatment compared with control ( Fig.…”

Section: Effects Of Snp and Nahs On The Nitrosative Status Of Citrus mentioning

confidence: 99%

Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants

Ziogas

Tanou

Belghazi³

et al. 2015

Plant Mol Biol

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Emerging evidence suggests that the gaseous molecules hydrogen sulfide (H2S) and nitric oxide (NO) enhances plant acclimation to stress; however, the underlying mechanism remains unclear. In this work, we explored if pretreatment of citrus roots with NaHS (a H2S donor) or sodium nitroprusside (SNP, a NO donor) for 2 days (d) could elicit long-lasting priming effects to subsequent exposure to PEG-associated drought stress for 21 d following a 5 d acclimation period. Detailed physiological study documented that both pretreatments primed plants against drought stress. Analysis of the level of nitrite, NOx, S-nitrosoglutahione reductase, Tyr-nitration and S-nitrosylation along with the expression of genes involved in NO-generation suggested that the nitrosative status of leaves and roots was altered by NaHS and SNP. Using a proteomic approach we characterized S-nitrosylated proteins in citrus leaves exposed to chemical treatments, including well known and novel S-nitrosylated targets. Mass spectrometry analysis also enabled the identification of 42 differentially expressed proteins in PEG alone-treated plants. Several PEG-responsive proteins were down-regulated, especially photosynthetic proteins. Finally, the identification of specific proteins that were regulated by NaHS and SNP under PEG conditions provides novel insight into long-term drought priming in plants and in a fruit crop such as citrus in particular.

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“…These results are comparable to the response of Panax ginseng exposed to 50 mM Cu where a similar increase in DAF-2T fluorescence and in nitrite concentration was observed in the adventitious roots . However, the specificity of DAFs reaction with NO has recently been questioned as DAF-reactive compounds were prevalently identified as reaction products of H 2 O 2 plus apoplastic peroxidase and only minor peak was detected for DAF-2T, a reaction product of DAFs with NO (Rumer et al, 2012). On the other hand, O-diamine moiety of DAF-2DA has been shown to be more selective for NO over NO 3 À , ONOO À , H 2 O 2 , and O 2 À under aerobic conditions and maintains a 5 nM NO detection limit (Pluth et al, 2011).…”

Section: Uranium Exposure Induces No Generationmentioning

confidence: 99%

Uranium exposure induces nitric oxide and hydrogen peroxide generation in Arabidopsis thaliana

Tewari

Horemans

Nauts

et al. 2015

Environmental and Experimental Botany

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DAF-fluorescence without NO: Elicitor treated tobacco cells produce fluorescing DAF-derivatives not related to DAF-2 triazol

Cited by 36 publications

References 25 publications

Defense induced by a bis-aryl methanone compound leads to resistance in potato against Phytophthora infestans

Defense induced by a bis-aryl methanone compound leads to resistance in potato against Phytophthora infestans

Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants

Uranium exposure induces nitric oxide and hydrogen peroxide generation in Arabidopsis thaliana

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