Uranium exposure induces nitric oxide and hydrogen peroxide generation in Arabidopsis thaliana

Uncovering the physiological and cellular effects of uranium on the root system of Arabidopsis thaliana

“…7). This is in agreement with a previous report showing that NO is produced in the roots of Arabidopsis plants challenged with U and that NO inducers or inhibitors of NO synthesis modulate the toxicity of the radionuclide (Tewari et al, 2015). The role of NO as a signal molecule during U stress is also supported by the demonstration that (i) an excess of Fe reduces root tip growth through a NO-mediated mechanism (Zhang et al, 2018), (ii) lignin is synthesized in response to high NO levels (Böhm et al, 2010;Corti Monzón et al, 2014), and (iii) NO influences the cell cycle in a dose-dependent manner, from stimulation to inhibition (Novikova et al, 2017).…”

“…Vanhoudt et al, 2008;Vanhoudt et al, 2011bc;Aranjuelo et al, 2014). Also, it has been shown that the production of hydrogen peroxide and nitric oxide (NO) is linked with the toxicity of the radionuclide in roots and leaves (Tewari et al, 2015). To investigate the pattern of ROS production in the primary root of young Arabidopsis seedlings challenged with U, we used the fluorogenic reagent 2′,7′-dichlorodihydrofluorescein diacetate that produces a green fluorescence when oxidized in the cytosol.…”

“…It has been shown that U can stimulate or inhibit the primary root growth as a function of its concentration and speciation (Misson et al, 2009). Also, U induces the accumulation of hydrogen peroxide and NO at the primary root apex (Tewari et al, 2015). These data are not sufficient per se to understand the cascade of cellular events that trigger changes in root development during U stress.…”

“…Uranium interferes with plant nutrition, photosynthesis, and induces an oxidative stress (e.g. Vanhoudt et al, 2011aVanhoudt et al, ,b,c, 2014Aranjuelo et al, 2014;Saenen et al, 2014Saenen et al, , 2015Tewari et al, 2015). Also, it has been shown that U perturbs iron and phosphate homeostasis and may lead to phosphate starvation (Misson et al, 2009;Doustaly et al, 2014;Berthet et al, 2018).…”

Exploitation Contradictions Concerning Multi-Energy Resources among Coal, Gas, Oil, and Uranium: A Case Study in the Ordos Basin (Western North China Craton and Southern Side of Yinshan Mountains)

“…Improper exploration of coal and uranium is likely to cause a major disaster to the local environment and ecosystem, especially for unscientific uranium mining. Uranium (U) is a nonessential and toxic heavy metal and radioactive element, and a possible leak of this element will certainly lead to the extinction of animals and plants [26,27]. Currently, to not import security and environment risks to local residents, it has been decided to abandon the original coal mining face under the uranium for the following main reasons: a) coal mining will induce a water downtrend phenomenon in the upper Jurassic confined aquifer, which is not allowed in the uranium mining horizon, and b) U 4+ will be oxidized into U 6+ under the strong oxidization environment of the leaching agent, dissociative uranium will migrate with the water flow, and it is possible for it to permeate into the lower coal mining face through the fractured cracks induced by strata caving, thus bringing radioactive contamination to the mine water and to the surface.…”