d-aspartate treatment in vitro improves mouse sperm fertility in young B6N mice

Raspa, Marcello; Paoletti, Renata; Mahabir, Esther; Scavizzi, Ferdinando

doi:10.1016/j.theriogenology.2020.02.031

Cited by 9 publications

(17 citation statements)

References 28 publications

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“…An increase in serum androstenedione and progesterone levels has also been detected [93,103,117,123,124]. Similarly, treatment with D-Asp in mice induced a significant increase in serum and testis LH, testosterone, and epitestosterone [18,19]. The increase in sex steroid hormone levels results from the effect of this D-amino acid on the transcription of the acute steroidogenic regulatory protein (StAR), which is a primary regulatory protein for the biosynthesis of testosterone in the testis as well as of steroidogenic enzymes (Figure 1) [117,124].…”

Section: D-asp Administration On the Hypothalamus-pituitary-testis Axismentioning

confidence: 82%

“…Experimental studies performed in various animal models have shown that D-Asp acts at all levels of the hypothalamic-pituitary-testis axis, which suggests that this D-amino acid plays a role also in vertebrate reproductive processes [16][17][18][19]91]. D-Asp has been found in substantial amounts in the mammalian pituitary gland [92,100,110,119,120].…”

Section: D-asp Administration On the Hypothalamus-pituitary-testis Axismentioning

confidence: 99%

“…Finally, experiments on isolated mouse spermatozoa showed that D-Asp improved total and progressive sperm motility [18,19]. Furthermore, treatment with a commercial mixture of Coenzyme Q10, zinc, and D-Asp (CZA) exerts a protective effect on bovine spermatozoa, since it counteracts the loss of motility by acting on the mitochondrial membrane potential (MMP) and DNA fragmentation [17,139].…”

Section: D-asp Administration On the Hypothalamus-pituitary-testis Axismentioning

confidence: 99%

“…Furthermore, D-Asp regulates the synthesis and release of testosterone by promoting the release of gonadotropin-releasing hormone (GnRH) in the hypothalamus and luteinizing hormone (LH) in the pituitary. D-Asp can promote animal reproduction also by directly activating spermatogonia proliferation and improving sperm quality [18,19].…”

Section: Introductionmentioning

confidence: 99%

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New Evidence on the Role of D-Aspartate Metabolism in Regulating Brain and Endocrine System Physiology: From Preclinical Observations to Clinical Applications

Usiello

Fiore

Rosa

et al. 2020

IJMS

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The endogenous amino acids serine and aspartate occur at high concentrations in free D-form in mammalian organs, including the central nervous system and endocrine glands. D-serine (D-Ser) is largely localized in the forebrain structures throughout pre and postnatal life. Pharmacologically, D-Ser plays a functional role by acting as an endogenous coagonist at N-methyl-D-aspartate receptors (NMDARs). Less is known about the role of free D-aspartate (D-Asp) in mammals. Notably, D-Asp has a specific temporal pattern of occurrence. In fact, free D-Asp is abundant during prenatal life and decreases greatly after birth in concomitance with the postnatal onset of D-Asp oxidase expression, which is the only enzyme known to control endogenous levels of this molecule. Conversely, in the endocrine system, D-Asp concentrations enhance after birth during its functional development, thereby suggesting an involvement of the amino acid in the regulation of hormone biosynthesis. The substantial binding affinity for the NMDAR glutamate site has led us to investigate the in vivo implications of D-Asp on NMDAR-mediated responses. Herein we review the physiological function of free D-Asp and of its metabolizing enzyme in regulating the functions of the brain and of the neuroendocrine system based on recent genetic and pharmacological human and animal studies.

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Section: D-asp Administration On the Hypothalamus-pituitary-testis Axismentioning

confidence: 82%

Section: D-asp Administration On the Hypothalamus-pituitary-testis Axismentioning

confidence: 99%

Section: D-asp Administration On the Hypothalamus-pituitary-testis Axismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

New Evidence on the Role of D-Aspartate Metabolism in Regulating Brain and Endocrine System Physiology: From Preclinical Observations to Clinical Applications

Usiello

Fiore

Rosa

et al. 2020

IJMS

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“…However, most of them have focused on in vitro strategies using antioxidants or agents that enhance membrane fluidity [ 8 , 9 ]. Recently, our group reported that the addition of D-Aspartate (D-Asp) to cryopreserved-thawed spermatozoa in vitro improves the fertility of young mice by increasing both the sperm capacitation and the acrosome reaction [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice

Raspa

Paoletti²,

Peltier

et al. 2022

Animals

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D-Aspartate (D-Asp) treatment improved the fertility of young male C57BL/6N mice in vivo revealing a direct role on capacitation, acrosome reaction, and fertility in vitro in young males only. We investigated whether the positive effect of D-Asp on fertility could be extended to adult males and evaluated the efficacy of a 2- or 4-week-treatment in vivo. Therefore, 20 mM sodium D-Asp was supplied in drinking water to males of different ages so that they were 9 or 16 weeks old at the end of the experiments. After sperm freezing, the in vitro fertilization (IVF) rate, the birth rate, hormone levels (luteinizing hormone (LH), epitestosterone, and testosterone), the sperm quality (morphology, abnormalities, motility, and velocity), the capacitation rate, and the acrosome reaction were investigated. Oral D-Asp treatment improves the fertilizing capability in mice regardless of the age of the animals. Importantly, a short D-Asp treatment of 2 weeks in young males elevates sperm parameters to the levels of untreated adult animals. In vivo, D-Asp treatment highly improves sperm quality but not sperm concentration. Therefore, D-Asp plays a beneficial role in mouse male fertility and may be highly relevant for cryorepositories to improve mouse sperm biobanking.

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d-aspartate and N-methyl-d-aspartate promote proliferative activity in mouse spermatocyte GC-2 cells

Falvo

Santillo

Baccari

et al. 2022

Reproductive Biology

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d-aspartate treatment in vitro improves mouse sperm fertility in young B6N mice

Cited by 9 publications

References 28 publications

New Evidence on the Role of D-Aspartate Metabolism in Regulating Brain and Endocrine System Physiology: From Preclinical Observations to Clinical Applications

New Evidence on the Role of D-Aspartate Metabolism in Regulating Brain and Endocrine System Physiology: From Preclinical Observations to Clinical Applications

Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice

d-aspartate and N-methyl-d-aspartate promote proliferative activity in mouse spermatocyte GC-2 cells

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