Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice

Raspa, Marcello; Paoletti, Renata; Peltier, Manon; Majjouti, Mohamed; Protti, Michele; Mercolini, Laura; Mahabir, Esther; Scavizzi, Ferdinando

doi:10.3390/ani12111350

Cited by 2 publications

(1 citation statement)

References 58 publications

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“…Additionally, the developmental ability of bovine sperm embryos treated with aspartic acid was significantly improved (45,46). Oral aspartate was shown to improve the fertility of young male C5BL/6N mice in vivo, increase freeze-thaw sperm quality in sexually immature mice, and play a direct role in the sperm capacitation process and acrosome reaction (47)(48)(49).…”

Section: Discussionmentioning

confidence: 99%

Metabolomic analysis of seminal plasma to identify goat semen freezability markers

Bai²,

Zhang³

et al. 2023

Front. Vet. Sci.

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Factors affecting sperm freezability in goat seminal plasma were investigated. Based on the total motility of thawed sperm, goats were divided into a high-freezability (HF) group with >60% total motility (n = 8) and a low-freezability (LF) group with <45% total motility (n = 8). Sperm and seminal plasma from the HF and LF groups were separated, HF seminal plasma was mixed with LF spermatozoa, LF seminal plasma was mixed with HF sperm, and the products were subjected to a freeze-thaw procedure. Semen from individual goats exhibited differences in freezability. HF semen had higher sperm motility parameters and plasma membrane and acrosome integrity after thawing; this difference could be related to the composition of seminal plasma. Seminal plasma from the HF and LF groups was evaluated using metabolomic analysis, and multivariate statistical analysis revealed a clear separation of metabolic patterns in the seminal plasma of goats with different freezability classifications. Forty-one differential metabolites were identified using the following screening conditions: variable importance in the projection > 1 and 0.05 < P-value < 0.1. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed significant enrichment of central carbon metabolism in cancer, protein digestion and absorption, aminoacyl-tRNA, and other pathways and significant differences in the abundance of seven differential metabolites, including L-glutamine, L-aspartate, L-arginine, phenylpyruvate, benzoic acid, ketoisocaproic acid, and choline between seminal plasma from the HF and LF groups (P-value < 0.05). These significantly differentially-expressed metabolites may be potential biomarkers for sperm freezability. L-glutamine, L-aspartate, and L-arginine may directly affect sperm freezability. Benzoic acid, ketoisocaproic acid, and choline may regulate sperm freezability by participating in anabolic processes involving phenylalanine, leucine, and phosphatidylcholine in sperm.

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Section: Discussionmentioning

confidence: 99%

Metabolomic analysis of seminal plasma to identify goat semen freezability markers

Bai²,

Zhang³

et al. 2023

Front. Vet. Sci.

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Potential role of mitochondria and endoplasmic reticulum in the response elicited by D-aspartate in TM4 Sertoli cells

Falvo,

Grillo,

Latino

et al. 2024

Front. Cell Dev. Biol.

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D-Aspartic Acid (D-Asp) affects spermatogenesis by enhancing the biosynthesis of the sex steroid hormones acting either through the hypothalamus-pituitary–testis axis or directly on Leydig cells. Recently, in vitro studies have also demonstrated the direct effects of D-Asp on the proliferation and/or activity of germ cells. However, although D-Asp is present in Sertoli cells (SC), the specific role of the amino acid in these cells remains unknown. This study investigated the effects of D-Asp on the proliferation and activity of TM4 SC, focusing on the mitochondrial compartment and its association with the endoplasmic reticulum (ER). We found that D-Asp enhanced the proliferation and activity of TM4 cells as evidenced by the activation of ERK/Akt/PCNA pathway and the increase in the protein levels of the androgen receptor. Furthermore, D-Asp reduced both the oxidative stress and apoptotic process. An increase in mitochondrial functionality and dynamics, as well as a reduction in ER stress, were also found in D-Asp-treated TM4 cells. It is known that mitochondria are closely associated with ER to form the Mitochondrial-Associated Endoplasmic Reticulum Membranes (MAM), the site of calcium ions and lipid transfer from ER to the mitochondria, and vice versa. The data demonstrated that D-Asp induced stabilization of MAM in TM4 cells. In conclusion, this study is the first to demonstrate a direct effect of D-Asp on SC activity and to clarify the cellular/molecular mechanism underlying these effects, suggesting that D-Asp could stimulate spermatogenesis by improving the efficiency of SC.

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Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice

Cited by 2 publications

References 58 publications

Metabolomic analysis of seminal plasma to identify goat semen freezability markers

Metabolomic analysis of seminal plasma to identify goat semen freezability markers

Potential role of mitochondria and endoplasmic reticulum in the response elicited by D-aspartate in TM4 Sertoli cells

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