d-aspartate and N-methyl-d-aspartate promote proliferative activity in mouse spermatocyte GC-2 cells

Falvo, Sara; Santillo, Alessandra; Baccari, Gabriella Chieffi; Cioffi, Federica; Fiore, Maria Maddalena Di

doi:10.1016/j.repbio.2021.100601

Cited by 11 publications

(10 citation statements)

References 65 publications

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“…We observed that the enhanced IVF rate and hormone level elicited by D-Asp is not associated with an increase in sperm concentration in both young and adult males. These results are in contrast to those from two studies, which reported that 200 µM D-Asp for 30 min, 2 h, or 4 h stimulates proliferative pathways in GC-1 [ 34 ] and in GC-2 cells, two mouse spermatocyte-derived cell lines [ 35 ]. Furthermore, a negative effect of 10 mM, but not 1 or 5 mM D-Asp, added to the culture medium on mitosis in premeiotic germ cells was reported [ 13 ].…”

Section: Discussioncontrasting

confidence: 99%

Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice

Raspa

Paoletti²,

Peltier

et al. 2022

Animals

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D-Aspartate (D-Asp) treatment improved the fertility of young male C57BL/6N mice in vivo revealing a direct role on capacitation, acrosome reaction, and fertility in vitro in young males only. We investigated whether the positive effect of D-Asp on fertility could be extended to adult males and evaluated the efficacy of a 2- or 4-week-treatment in vivo. Therefore, 20 mM sodium D-Asp was supplied in drinking water to males of different ages so that they were 9 or 16 weeks old at the end of the experiments. After sperm freezing, the in vitro fertilization (IVF) rate, the birth rate, hormone levels (luteinizing hormone (LH), epitestosterone, and testosterone), the sperm quality (morphology, abnormalities, motility, and velocity), the capacitation rate, and the acrosome reaction were investigated. Oral D-Asp treatment improves the fertilizing capability in mice regardless of the age of the animals. Importantly, a short D-Asp treatment of 2 weeks in young males elevates sperm parameters to the levels of untreated adult animals. In vivo, D-Asp treatment highly improves sperm quality but not sperm concentration. Therefore, D-Asp plays a beneficial role in mouse male fertility and may be highly relevant for cryorepositories to improve mouse sperm biobanking.

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Section: Discussioncontrasting

confidence: 99%

Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice

Raspa

Paoletti²,

Peltier

et al. 2022

Animals

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show abstract

“…Antioxidant drugs can alleviate function impairment of sperm mitochondria induced by proline catabolism disorder ( Yen and Curran 2021 ). More studies of amino acids have shown that homocysteine level is increase d in sperm from patients with severe disruption of semen proteins ( Kralikova, et al, 2017 ), that d -Aspartate (D-Asp) and N-methyl- d -aspartate (NMDA) promote spermatogenesis by stimulating the biosynthesis of sex steroid hormones ( Di Fiore, et al, 2014 ), that D-Asp and NMDA can increase the expression of OXPHOS complex ( Falvo, et al, 2022 ). Additionally, tyrosine phosphorylation is regulated by redox signaling ( Chiarugi and Cirri 2003 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of genes related to sexual differentiation and sterility in embryonic gonads of Mule ducks by transcriptome analysis

Yang

Li²,

Ye³

et al. 2022

Front. Genet.

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The key genes of avian gonadal development are of great significance for sex determination. Transcriptome sequencing analysis of Mule duck gonad as potential sterile model is expected to screen candidate genes related to avian gonad development. In this study, the embryonic gonadal tissues of Mule ducks, Jinding ducks, and Muscovy ducks were collected and identified. Six sample groups including female Mule duck (A), male Mule duck (B), female Jinding duck (C), male Jinding duck (D), female Muscovy duck (E), and male Muscovy duck (F) were subjected to RNA sequencing analysis. A total of 9,471 differential genes (DEGs) and 691 protein-protein interaction pairs were obtained. Totally, 12 genes (Dmrt1, Amh, Sox9, Tex14, Trim71, Slc26a8, Spam1, Tdrp, Tsga10, Boc, Cxcl14, and Hsd17b3) were identified to be specifically related to duck testicular development, and 11 genes (Hsd17b1, Cyp19a1, Cyp17a1, Hhipl2, Tdrp, Uts2r, Cdon, Axin2, Nxph1, Brinp2, and Brinp3) were specifically related to duck ovarian development. Seven genes (Stra8, Dmc1, Terb1, Tex14, Tsga10, Spam1, and Plcd4) were screened to be specifically involved in the female sterility of Mule ducks; eight genes (Gtsf1, Nalcn, Tat, Slc26a8, Kmo, Plcd4, Aldh4a1, and Hgd) were specifically involved in male sterility; and five genes (Terb1, Stra8, Tex14 Tsga10 and Spam1) were involved in both female and male sterility. This study provides an insight into the differential development between male and female gonads of ducks and the sterility mechanism of Mule ducks through function, pathway, and protein interaction analyses. Our findings provide theoretical basis for the further research on sex determination and differentiation of birds and the sterility of Mule ducks.

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“…However, in aging, ERK activation controls mitochondrial fusion and StAR localization in mitochondria results in steroidogenesis disturbance in Leydig cells. Therefore, the role of ERK1/2 in influencing steroidogenesis is still being explored and articulated, as it has been found to be inhibitory in some studies, while other investigations have shown a beneficial influence of MAPK activation on the synthesis of sex steroids [ 62 , 63 ].…”

Section: Effects Of Bpa and Its Analogues On Egfr-mapk-erk Signaling ...mentioning

confidence: 99%

Knowledge Gap in Understanding the Steroidogenic Acute Regulatory Protein Regulation in Steroidogenesis Following Exposure to Bisphenol A and Its Analogues

et al. 2022

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The use of bisphenols has become extremely common in our daily lives. Due to the extensive toxic effects of Bisphenol A (BPA), the industry has replaced this endocrine-disrupting chemical (EDC) with its analogues, which have been proven to decrease testosterone levels via several mechanisms, including targeting the steroidogenic acute regulatory (StAR) protein. However, when exposed to BPA and its analogues, the specific mechanism that emerges to target StAR protein regulations remains uncertain. Hence, this review discusses the effects of BPA and its analogues in StAR protein regulation by targeting cAMP-PKA, PLC-PKC, EGFR-MAPK/ERK and Ca2+-Nur77. BPA and its analogues mainly lead to decreased LH in blood and increased ERK expression and Ca2+ influx, with no relationship with the StAR protein regulation in testicular steroidogenesis. Furthermore, the involvement of the cAMP-PKA, PLC-PKC, and Nur77 molecules in StAR regulation in Leydig cells exposed to BPA and its analogues remains questionable. In conclusion, although BPA and its analogues have been found to disrupt the StAR protein, the evidence in connecting the signaling pathways with the StAR regulations in testicular steroidogenesis is still lacking, and more research is needed to draw a solid conclusion.

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d-aspartate and N-methyl-d-aspartate promote proliferative activity in mouse spermatocyte GC-2 cells

Cited by 11 publications

References 65 publications

Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice

Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice

Identification of genes related to sexual differentiation and sterility in embryonic gonads of Mule ducks by transcriptome analysis

Knowledge Gap in Understanding the Steroidogenic Acute Regulatory Protein Regulation in Steroidogenesis Following Exposure to Bisphenol A and Its Analogues

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