Cа2+ transporting systems in secretory cells of the rat exorbital lacrimal gland. І. Ca2+ pumps of plasma membrane and endoplasmic reticulum

The role of intracellular organelles in Ca2+-homeostasis in secretory cells of the lacrimal gland

“…It should be noted that the results obtained by the ATPase activity measurement of permeabilized cell under the influence of these inhibitors [34] are in good agreement with the results obtained from measuring changes of the Ca 2+ content in cells [35]. Reduction of Ca 2+ content in permeabilized cells by thapsigargin (1 µM) -specific SERCA inhibitor was the same as by eosin Y (5 mM).…”

“…Reduction of Ca 2+ content in permeabilized cells by thapsigargin (1 µM) -specific SERCA inhibitor was the same as by eosin Y (5 mM). In contrast to permeabilized cells, the Ca 2+ content in intact cells did not change under the influence of eosin Y (5-20 µM), indicating the PMCA inhibition [35].…”

“…In our first article, published in Studia Biologica [35], we addessed the identification and features of SERCA and PMCA. The purpose of this review is to analyze the contribution of other intracellular Ca 2+ -transport systems in the Ca 2+ -homeostasis of the lacrimal gland secretory cells.…”

“…The only system that provides Ca 2+ transport from the cytosol to the endoplasmic reticulum is SERCA (Ca 2+ , Mg 2+ -ATPase of endoplasmic reticulum) [41]. Primarily, thapsigargin [54] and eosin Y [30,35,53] belong to the inhibitors of Ca 2+ -pumps. It was shown that 2-APB is also capable to inhibit SERCA of the lacrimal gland secretory cells [31].…”

The role of mitochondrial uniporter in calcium-homeostasis of the exorbital lacrimal gland secretory cells

“…Додавання тапсигаргіну до інтактних і пермеабілізованих клітин спричиняло ста-тистично достовірне зменшення вмісту Са 2+ у них на 17,72 ± 5,82 і 21,06 ± 6,52 % відпо-відно -за рахунок пригнічення кальцієвої помпи ендоплазматичного ретикулума, що узгоджується з отриманими раніше даними [9]. Як в інтактних, так і у пермеабілізованих клітинах сумарні ефекти рутенію червоного і тапсигаргіну становили 29,62 ± 5,37 i 33,15 ± 7,73 % відповідно (Р < 0,01) і були такими самими, як сума ефектів кожної з цих речовин окремо, тобто адитивними (Р а = 0,74 і 0,19).…”