The role of mitochondrial uniporter in calcium-homeostasis of the exorbital lacrimal gland secretory cells

The role of intracellular organelles in Ca2+-homeostasis in secretory cells of the lacrimal gland

“…This suggests on the influence of these two inhibitors Ca 2+ is passively released from different stores. Similarly, effects of IP 3 (2 µM) and ruthenium red in permeabilized cells, and M-cholinergic receptors agonist carbacholine (10 µM) and ruthenium red on intact cells were additive [37]. Beside this, adding carbacholine to the polarographic chamber caused an intensification of respiration of the studied cells.…”

“…Ruthenium red (10 µM) -an inhibitor of Ca 2+ -uniporter and thapsigargin (1 µM)an inhibitor of SERCA reduced the Ca 2+ content in intact and permeabilized cells of lacrimal glands. The total effect of ruthenium red and thapsigargin on Ca 2+ content in cells was additive [37]. This suggests on the influence of these two inhibitors Ca 2+ is passively released from different stores.…”

“…However, the effects of ryanodine (0.1 µM) and ruthenium red on total Ca 2+ -content in the lacrimal cells were non-additive ( Fig. 5) [37].…”

“…The unaccounted factor amounted to only 3.49 % [32]. Simultaneous activation of RyRs and IP 3 Rs in these cells caused Ca 2+ release from the same store, since the effects of IP 3 (2 µM) and ryanodine (100 nM) on the total Ca 2+content in permeabilized secretory cells were statistically significantly non-additive [37].…”

“…For its inhibition ruthenium red is often used [20,28]. Today the functional activity of mitochondrial Ca 2+ -uniporter in the lacrimal gland secretory cells is confirmed [4] and its interaction with other Ca 2+ -transport systems in cells was studied on basis of the estimation of additivity of the effects of appropriate inhibitors [37].…”