Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells

Abstract

This study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements. For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h. The cytotoxicity of the same materials was also evaluated in a dentin barrier test device using three-dimensional cell cultures of bovine pulp-derived cells. The results of the cytotoxicity studies with two-dimensional cultures of bovine dental pulp-derived cells revealed that cell survival with the extracts of Rely X Temp E, Provicol, PreVISION CEM, and Rely X Temp NE was 89.1%, 84.9%, 92.3%, and 66.8%, respectively. Rely X Temp NE and Provicol showed cytotoxic effects on bovine dental pulp-derived cells (P < 0.05). The results of the dentin barrier test revealed that cell survival with the above-mentioned temporary cement was 101.5%, 91.9%, 93.5%, and 90.6%, respectively. None of the temporary luting cements significantly reduced cell survival compared with the negative control in the dentin barrier test (P > 0.05). Biologically active materials released from temporary luting cements may not influence the dentine-pulp complex if the residual dentine layer is at least 0.5 mm thick.

Citotoxicity evaluation of three dental adhesives on vero cells in vitro

“…Although the components (HEMA, TEGDMA, phosphate monomer) might seem toxic after the initial monomer release ( 22 ), they did not interfere in cell growth, which may have led them to proliferate as they usually would in favorable conditions. Similar findings have been observed in previous studies using Easy Bond, with percentages of cell viability from 103.37% to 110.39%, depending on the methodology ( 23 , 24 ). Cell viability over 100% observed in some experimental groups can be attributed to the accumulation of cell waste products and metabolites in the control negative cells, as the media had not been changed for the last 48h whilst our cells seemed almost 100% confluent at 24h.…”

Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis

“…The present study demonstrated that Ultratemp, which contains zinc oxide, was cytotoxic to bDPCs. The test design and investigation conditions like as xCELLigence system and bDPCs cultures of present study are different from Ülker et al 25 However, Kwon et al 21 studied cytotoxicity of commercially available ZOE and ZONE cements by MTT test. They demonstrated that both ZOE and ZONE cements showed varying degrees of cytotoxicity in accordance with our data.…”

“…Ülker et al 25 reported that zinc oxide-containing temporary cement is not cytotoxic to bovine pulp-derived cells when tested using MTT method, even if dentin barrier does not exist. The present study demonstrated that Ultratemp, which contains zinc oxide, was cytotoxic to bDPCs.…”

Comparison of the Effects of Cytotoxicity and Antimicrobial Activities of Self-adhesive, Eugenol and Noneugenol Temporary and Traditional Cements on Gingiva and Pulp Living Cells

“…Also, Malkoç et al 24 reported that temporary cements with and without eugenol showed cytotoxic effects at different ratios in their study in which they investigated the cytotoxicity of temporary cements on animal pulp cells. However, Ülker et al 27 have reported that the eugenol-containing temporary cement is not cytotoxic to animal-derived pulp cells without the dentin barrier.…”