Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis

Abstract

PURPOSETo evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs).MATERIALS AND METHODSFour different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 µL of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments.RESULTSAll tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results.CONCLUSIONThe cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.

Cytotoxic effects of contemporary bulk-fill dental composites: A real-time cell analysis

“…Cell types used for in vitro cytotoxicity studies could play a significant role in the outcomes. Pulp fibroblasts, gingival fibroblasts, cells obtained from cattle dental papilla, odontoblast-like cells collected from mice and human dental pulp stem cells are most commonly used in studies investigating the cytotoxicity of dental materials 9,[13][14][15][24][25][26] . In the present study, L929 cells were selected, as they are easily available and have efficient in-vitro growth due to their homogenous morphology and growth characteristics 9) .…”

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In Vitro Cytotoxicity of Two Categories of Dental Cements

“…It may be suggested that the concentration of HEMA could be one of the factors responsible for the higher cytotoxicity of Fuji PLUS compared to Fuji I . (12,25,26) However, the concentrations of HEMA that can diffuse into the pulpal tissue are significantly lower than the concentrations which cause acute toxicity. Therefore, for RM-GIC, the leaching of the combination of toxic components including resin monomers and fluoride ions can be suspected.…”

Cytotoxicity evaluation of eluates from universal adhesives by real-time cell analysis

“…Unlike above-mentioned endpoint approaches, realtime assay systems provide for the tracking of cellular growth over the entire time course of an experiment. RTCA was used by researchers to assess the cytotoxicity of different dental materials 23,55,56) . One of the RTCA devices is iCELLigence system (ACEA Biosciences, San Diego, CA, USA).…”