Cytotoxicity evaluation of eluates from universal adhesives by real-time cell analysis

Abstract

The aim of this study was to evaluate the cytotoxicity of universal adhesives on L929 mouse fibroblast cell line by using a realtime cell analysis. In order to obtain extract, six different cured dental adhesives were immersed in Dulbecco's Modified Eagle's Medium (DMEM) at 37°C for 24 h. A real-time cell analysis system was used to assess cytotoxicity of the dental adhesives. After seeding 25,000 cells/300 μL/well cell suspensions into the wells of an e-plate, fibroblasts were exposed to extracts of tested adhesives at varying dilutions (1:1, 1:2, and 1:10) and observed at every 30 min intervals for 72 h. Three-way ANOVA one factor repeated measures were used to analyze the results (α=0.05). All tested adhesives induced cell viability loss, cell morphology alteration, and cell death depending on extract concentration and time. Cell viability of L929 cells to between 44 and 10% for 1:1 diluted extracts, at 72 h, when compared to the negative control.

Functional evaluation of mineral trioxide aggregate cement with choline dihydrogen phosphate

“…This result may be due to the effect of the CDHP being added to the MTA cement. L929 cells were observed to be elongated into a spindle shape with a size of about 100 μm, which is a typical morphology of L929 as an adhesive cell 22) . Elliott et al 17,18) also reported a decrease in cell viability above a certain level with increasing CDHP concentrations, but no decrease in cell viability was observed in the 0.2-0.8 g/mL range in the present study.…”

Long-term, real-time and label-free live cell image processing and analysis based on a combined algorithm of CellPose and watershed segmentation

“…Moreover, these methods cannot meet the needs of high-throughput data analysis because of the cumbersome relevant statistical analysis [ 6 ]. In order to solve these problems, researchers have proposed a new approach that can provide detailed information on the entire lifecycle of cells by observing and tracking them in real-time and in situ [ [7] , [8] , [9] ]. This method not only greatly deepens our understanding of the physiological properties of cells but can also accelerate drug development processes as it allows us to assess drug cytotoxicity directly and rapidly [ 3 ].…”