Cytotoxicity of tamoxifen in normal and tumoral cell lines and its ability to induce cellular transformation in vitro

Petinari, Leandro

doi:10.1016/j.cellbi.2004.04.008

Cited by 37 publications

(35 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…According to the latest report from the International Agency for Research on Cancer/WHO, the incidence was estimated at 12.7 million new cases annually, with cancer causing the deaths of 7.6 million people worldwide per year 8 . Tamoxifen (TAM) is a synthetic no steroidal antiestrogen, it is commonly prescribed to treat patients with breast cancer 9 . TAM belongs to the family of selective modulators of estrogen receptors.…”

Section: Introductionmentioning

confidence: 99%

Effect of the o-methyl catechols apocynin, curcumin and vanillin on the cytotoxicity activity of tamoxifen

Pedroso

Fávero

Camargo

et al. 2012

Journal of Enzyme Inhibition and Medicinal Chemistry

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Effect of the o-methyl catechols apocynin, curcumin and vanillin on the cytotoxicity activity of tamoxifen

Pedroso

Fávero

Camargo

et al. 2012

Journal of Enzyme Inhibition and Medicinal Chemistry

View full text Add to dashboard Cite

“…Tamoxifen may be having same effect in colon carcinoma. Tamoxifen also demonstrates its effect through growth factors and immune system, and thus, it provides certain benefits for estrogen-neutral patients (11)(12)(13). The aim of our study is to investigate the effects of quercetin and tamoxifen on apoptosis in CaCo-2 human colon adenocarcinoma cell line.…”

Section: Introductionmentioning

confidence: 99%

“…Quercetin can be also modulate estrogen receptor activation (6)(7)(8)(9)(10). Another agent we used in our study, tamoxifen is a non-steroidal anti-estrogen drug commonly used in the treatment of estrogen receptor positive breast cancer (11,12). Among the effect mechanisms of tamoxifen is to bind to estrogen receptor in order to prevent the recurrence of breast carcinoma and to inhibit the growth of cancer cells.…”

Section: Introductionmentioning

confidence: 99%

How Does Quercetin and Tamoxifen Affect the Apoptosis of Colon Cancer Cells?

Coşan

Soyocak

Başaran

et al. 2015

Osmangazi̇ Journal of Medicine

View full text Add to dashboard Cite

It is a known fact that compounds found in various vegetables and fruits taken by diet may be of help in treating cancer. A polyphenolic compound, quercetin was reported to have a possible impact on some types of cancer through a variety of molecules. However, there are ongoing studies to prove the effect mechanisms and it has yet to be clarified. Tamoxifen is a nonsteroidal anti-estrogen drug used in the treatment of estrogen-positive breast cancer. We already have some information regarding occasional use of tamoxifen in treatment of some cancers other than breast cancer, and thus we began investigating the effects of these two components on apoptosis in colon cancer cells in our study considering that it may also be effective in colon cancer. The effect of quercetin and tamoxifen on apoptotic index was evaluated by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method at the 24th, 48th, and 72nd hours. In colon cancer cells, the effects of various concentrations (25, 50, and 100 µM) of quercetin and tamoxifen on apoptotic index in colon cancer cells were established. Apoptotic index increased in groups that were administered quercetin and tamoxifen compared to control groups. The current study shows that quercetin and tamoxifen may play an important role in the treatment of cancer by inducing apoptosis in colon adenocarcinoma. KEYWORD: Apoptosis, CaCo-2, Quercetin, Colon Cancer, Tamoxifen KOLON KANSER HÜCRELERİNİN APOPTOZUNU KERSETİN VE TAMOKSİFEN NASIL ETKİLER?ÖZET: Diyetle alınan çeşitli sebze ve meyvelerde bulunan bileşiklerin kanser tedavisine yardımcı olabileceği bilinmektedir. Polifenolik bir bileşik olan kersetinin de bazı kanserlerde çeşitli moleküller üzerinden etkili olabildiği hakkında mevcut araştırmalar vardır. Ancak etki mekanizmalarının belirlenmesi için çalışmalar devam etmekte olup, tam olarak açıklığa kavuşmamıştır. Tamoksifen ise östrojen pozitif meme kanserinin tedavisinde kullanılan bir nonsteroid anti-östrojen ilaçtır. Zaman zaman meme kanseri dışında diğer bazı kanserlerde de kullanıldığı hakkında çeşitli bilgilere sahip olduğumuz tamoksifenin, kolon kanserinde de etkili olabileceğini düşünerek araştırmamızda kolon kanser hücrelerinde bu iki bileşenin apoptoz üzerine etkilerini incelemeyi amaçladık. Kersetin ve tamoksifenin 24, 48 ve 72. saatlerde apoptotik indekse olan etkileri TUNEL metodu belirlendi. Kolon kanser hücrelerinde kersetin ve tamoksifenin çeşitli konsantrasyonlarda (25, 50 ve 100 µM) apoptotik indekse olan etkileri belirlendi. Bulgular: Apoptotik indeksin kersetin ve tamoksifen uygulanan gruplarda kontrol grubuna göre arttığı bulundu. Apoptotic index increased in groups that were administered quercetin and tamoxifen compared to control groups. Kolon adeno karsinomada kersetin ve tamoksifenin apoptozu indükleyerek kanser tedavisinde önemli bir rol oynayabileceğini bulunmuştur.

show abstract

“…Tamoxifen is a non-steroidal anti-estrogen drug widely used in the treatment of patients with estrogen receptor (ER)-positive breast cancer (12).…”

Section: Introductionmentioning

confidence: 99%

“…By binding to ER, tamoxifen decreases the expression of estrogen-dependent gene stimulated by estrogen (12,13).…”

Section: Introductionmentioning

confidence: 99%

Evaluation Of Effects Of Quercetin (3,3',4',5,7-pentohidroxyflavon) On Apoptosis And Telomerase Enzyme Activity In Mcf-7 And Nih-3t3 Cell Lines To Compared With Tamoxifen

Hatipoglu¹,

Başaran²,

Dikmen³

et al. 2010

TUTFD

View full text Add to dashboard Cite

Objective: Quercetin has been shown to inhibit the proliferation of cancer cells. Tamoxifen is used for breast cancer. In this study, we have aimed to investigate the effects of quercetin and tamoxifen on telomerase enzyme activity and apoptosis in two cell lines. Methods:In this study, 10, 50 and 100 µM of quercetin and tamoxifen were used to treat MCF-7 and NIH-3T3. Apoptosis was determined by the TUNEL method (Terminal Deoxynucleotide Transferase dUTP Nick End Label) and telomerase enzyme activity was determined by ELISA (Enzyme-Linked Immunosorbent Assay).Results: In the NIH3T3 cell line, only 100 µM quercetin and tamoxifen induced significant apoptosis. In the MCF-7 cell line 10 µM and 100 µM quercetin in the 24 th hour and 100 µM quercetin in the 72 nd hour induce apoptosis. In 24 th and 48 th hours, 50 µM tamoxifen and 100 µM tamoxifen in the 24 th and 48 th hours induce apoptosis in the MCF-7 cell line. In MCF-7 and NIH-3T3 cell lines, all doses of quercetin and tamoxifen reduced telomerase enzyme activity compared to the control group. Conclusion:In this study, it was shown that quercetin has similar effects to tamoxifen. However quercetin induces apoptosis more than decreasing telomerase enzyme activities, being different from tamoxifen. We hope that the findings will assist in developing new therapeutic pathways for preventing breast cancer. However, there should be many more studies in order to discover quercetin and other potential drugs.

show abstract

Cytotoxicity of tamoxifen in normal and tumoral cell lines and its ability to induce cellular transformation in vitro

Cited by 37 publications

References 25 publications

Effect of the o-methyl catechols apocynin, curcumin and vanillin on the cytotoxicity activity of tamoxifen

Effect of the o-methyl catechols apocynin, curcumin and vanillin on the cytotoxicity activity of tamoxifen

How Does Quercetin and Tamoxifen Affect the Apoptosis of Colon Cancer Cells?

Evaluation Of Effects Of Quercetin (3,3',4',5,7-pentohidroxyflavon) On Apoptosis And Telomerase Enzyme Activity In Mcf-7 And Nih-3t3 Cell Lines To Compared With Tamoxifen

Contact Info

Product

Resources

About