Cytotoxicity of Insecticide Temik 15G (Decarb) in Mitotic and Meiotic Cells of Vicia faba Plant.

Ghareeb, A.; George, N. M.

doi:10.1508/cytologia.62.259

Cited by 9 publications

(5 citation statements)

References 7 publications

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“…A significance increase in the percentage of abnormalities was recorded at 30 and 60% concentrations 12 and 24 h treatment . Strongly we could conclude that there is strong negative relationship between MI and percentage of abnormalities as previously recorded for many extracts treatments (Banerjee 1992, Abderrhman 1998, Anis and Wani 1997, herbicides (Badr et al 1985) and insecticide (Ghareeb and George 1997).…”

supporting

confidence: 71%

Cytogenetic Effects of Calotropis procera Extract on Vicia faba L.

Haroun

Shehri

2001

CYTOLOGIA

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Summary The present investigation study the morphological and cytogenetic effects of Calotropis procera extract on Vicia faba L. The control plants being normal while treated ones are significantly affected. Four concentrations (5, 15, 30, 60%) of this extract were applied for 3 duration time. For treated seeds, low doses caused increase in mitotic indix (MI) and exhibit stimulatory effect on percentage of germination and plant height. On the other hand high concentratiosn gave rise to substantial reduction in MI, percentage of germination and plant height and all were found dose and time dependent. In contrast, all parameters tested for treated roots were found negatively affected with no exception and found more harmful and effective compared to seed treatment experiment. Various types of mitotic and meiotic abnormalities were observed. Their percentage of these abnormalities was also found dose and time dependent. Stickiness type was found more frequent compared with other types. The extract also affect chromosome association during meiosis. Chromosome pairing, chiasma frequency and pollen fertility were found negatively affected and dose dependent. The present data confirm the use of this extract as insect control at high concentrations is harmful to plants as non-target organism. It also recommend the use of lower concentration (15%), which has a significant affect on insect with very low side effects on plants. Key words Calotropis procera extract, Cytogenetic effects, Vicia faba The use of synthetic chemicals for plant protection and their cytogenetic effects have been well documented (Shehab 1980, Mohammed 1986, Badr et al. 1985, Qureshi et al. 1988. No doubt, that most of synthetic chemical used surround the world resulted in disturbances of environment and lethal on non target organisms e.g., plant, animal etc. For these reasons, it has become necessary to search alternative natural compounds for environmental safety.Extracts of many plants have been tested for the above reasons (El Gammal et al. 1988, Nassar 1995, Nassar et al. 1997, Soliman and El Sherif 1995, Mohammed 1986. Although many studies were carried on the cytogenetic effects of many plant extracts (Kumar and Kumar 1972, Kabarity and Malallah 1980, Adams and Rashad 1984, Abderrhman 1998, Shapira et al. 1989, Li et al. 1995, Abou El Kheir and Abou El Kheir 1992, Omani et al. 1996, El Khodary 1980, and some others are still in need for such investigation.Extract of Calotropis procera (Family Asclepiadaceae) has been used as botanical insect growth inhibitor by Nassar (1995) and Giridhar et al. (1984). The extract shows effective results as insecticide at certain concentrations.The aim of this study is to investigate the effect of different concentrations (5, 15, 30, 60%) of this extract on percentage of germination, plant growth and cytogenetic of Vicia faba as non target organism. Parameters measured were plant height, percentage of germination, mitotic index, aberrations and mitotic behaviour.

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supporting

confidence: 71%

Cytogenetic Effects of Calotropis procera Extract on Vicia faba L.

Haroun

Shehri

2001

CYTOLOGIA

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“…The presence of micronuclei, bridges, unequal distribution or multipolar separations can directly lead to the production of aneuploid cells (Novak, 1981). Ghareeb and George (1997) further reported that these abnormalities occur as a result of partial spindle failure. Amer and Ali (1974) had earlier noted that micronuclei result from lagging chromosomes at anaphase or telophase stages.…”

Section: Discussionmentioning

confidence: 99%

Mutagenic Potentials of the Sterilizing Fluid – Puritil on Root Tip Mitosis of <i>Allium cepa</i>

Akaneme¹,

Iyioke²

2009

Bio-Research

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The mutagenic potentials of puritil which is used for sterilizing babies feeding utensils were assessed using the Allium cepa root meristem assay. The sterilizing fluid was found to exhibit both mitoclassic and chromatoclassic effects and thus induced a number of abnormalities. These abnormalities included disturbed interphase involving nuclear lesions and nuclear dissolutions, micronuclei and binucleate cells, sticky metaphase, disturbed anaphase involving unequal distribution of chromosomes and sticky bridges, and precocious chromosomes. These increased with increase in concentration and duration of treatment. Thus the mutagenic potentials of puritil exist and it should be of great concern since babies are exposed to it for at least the first two years of life.

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“…Fragments, micronuclei and tripolar cells were also observed. These types of anomalies were observed also in the mitosis of Vicia faba after treatment with other chemicals, insecticides (Amer andFarah 1985, Ghareeb andGeorge 1997) and heavy metals (Georg 1998).…”

Section: Effect On Mitosismentioning

confidence: 87%

“…The molluscicide used in this study resemble other chemicals regarding the ability to inhibit cell division, for instance trichlorphon (Amer and Ali 1968) and Temik (Ghareeb and George 1997). Van't Hoff (1968) suggested that the inhibition of mitotic activity by chemical compounds is due to an increase in the G2 period.…”

Section: Effect On Mitosismentioning

confidence: 98%

The Mutagenic Activities of Uccmaluscide Using Vicia faba as a Biological System.

George

El-Haleem

2001

CYTOLOGIA

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Summary The effect of the molluscicide "Uccmaluscide" on mitosis and meiosis of Vicia faba has been studied. Mitotic activity of Vicia faba root tip cells decreased and produced different kinds of chromosomal abnormalities after treatment with different concentrations. Vicia faba plants were sprayed at flowering stage with the different concentrations of uccmaluscide solution. The percentage of abnormal pollen mother cells increased as the concentration increased. The most dominant types of abnormalities were stickiness, bridges, fragments of chromosomes, disturbance of mitotic stage and littlepolar or multipolar cells. Less than 4 spores were the dominant type of abnormalities observed in the quartet stage. Nonviable pollen grains were observed in addition to the small pollen grains.

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Cytotoxicity of Insecticide Temik 15G (Decarb) in Mitotic and Meiotic Cells of Vicia faba Plant.

Cited by 9 publications

References 7 publications

Cytogenetic Effects of Calotropis procera Extract on Vicia faba L.

Cytogenetic Effects of Calotropis procera Extract on Vicia faba L.

Mutagenic Potentials of the Sterilizing Fluid – Puritil on Root Tip Mitosis of <i>Allium cepa</i>

The Mutagenic Activities of Uccmaluscide Using Vicia faba as a Biological System.

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