Cytotoxicity of chlorpyrifos to human liver hepatocellular carcinoma cells: effects on mitochondrial membrane potential and intracellular free Ca<sup>2+</sup>

Zhou, Chune; Li, Xiaoyü

doi:10.1080/15569543.2017.1386686

Cited by 15 publications

(9 citation statements)

References 30 publications

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“…Numerous studies have shown that increased levels of pollutants both in the environment and in foods effect on liver tissue . One of the most important pollutants used in agriculture is CPF, which has increased penetration into the body due to inappropriate life style and can affect most tissues, including the liver . Because, due to the unique position of the liver in the circulatory system and chemical transmission, the most important risk of CPF toxicity is on this tissue .…”

Section: Discussionmentioning

confidence: 99%

Aerobic exercise and eugenol supplementation ameliorated liver injury induced by chlorpyrifos via modulation acetylcholinesterase activation and antioxidant defense

Nikbin

Tajik

Allahyari

et al. 2020

Environmental Toxicology

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The primary metabolize of chlorpyrifos (CPF) is in the liver tissue, which it can cause oxidative damage and apoptosis in liver cells. The use of exercise with antioxidant supplements could have a protective effects in the liver tissue especially by improve mitochondria function. The aim of the present study was to investigate the protective effect of aerobic exercise and eugenol (Eu) supplementation on destructive effects of CPF in liver tissue. Sixty-four adult male albino rats were randomly divided into eight groups (eight rats in each group). Four experimental groups received intraperitoneal injection of either 3.0 mg/kg body weight CPF in dimethyl sulfoxide for six consecutive weeks. Aerobic exercise was performed 5 days per week over 4 weeks for exercise groups. Finally, the animals were sacrificed for the histomorphometric analysis and biochemical measurement in the liver tissue. The result of this study show that consumption of CPF alone, caused collagen deposition, increased apoptosis, tumor necrosis factor α, malondialdehyde, and decreased catalase, superoxide dismutase, acetylcholinesterase (AChE) compared to control and exercise groups

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Section: Discussionmentioning

confidence: 99%

Aerobic exercise and eugenol supplementation ameliorated liver injury induced by chlorpyrifos via modulation acetylcholinesterase activation and antioxidant defense

Nikbin

Tajik

Allahyari

et al. 2020

Environmental Toxicology

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“…The literature describes the mechanisms involved in the genotoxic effect on different human cells, both normal and pathological. One of such studies has demonstrated that CPF treatment increased cell membrane injury in HepG2 cells, increased the intracellular free Ca 2+ concentration, and decreased the mitochondrial transmembrane potential, in addition to inducing slight apoptosis (Zhou & Li, 2018). CPFinduced apoptotic effect was also observed in three testicular cell lines through the generation of reactive oxygen species at a concentration range of 3.5-17.5 μg mL -1 (Chen et al, 2018), as well as in HEK293 human embryonic kidney cells, but in this case, it was intermediated by IL6 (van Emmon, Pan, & van Breukelen, 2018).…”

Section: Discussionmentioning

confidence: 99%

Chlorpyrifos induces genotoxic effects in human leukocytes in <i>vitro</i> at low concentrations

Serpa

Schmitt

Zuravski

et al. 2019

Acta Sci. Health Sci.

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Chlorpyrifos (CPF) is an organophosphate widely marketed as a pesticide. However, only few studies have investigated its genotoxic potential, especially in human tissues or cells, or the minimal concentration capable of inducing damage. This study analyzed the in vitro genotoxic potential of CPF in cultured human leukocytes at concentrations of 3, 35, and 350 μg mL-1. Results demonstrated that the two highest concentrations exhibited an increase (~2-fold) in the micronucleus frequency (p < 0.01), the number of numerical chromosomal instabilities (~10-12 times, p < 0.05), and the number of apoptotic cells (~10-to 20-fold, p < 0.001). These results demonstrate the genotoxic potential of CPF and could open new discussions regarding its safety and toxicology.

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“…The stability of mitochondrial membrane preserves the dynamic equilibrium of intracellular free calcium concentrations, and their decline may also lead to apoptosis (29). A recent study (30) showed higher free Ca 2+ concentrations, higher plasma membrane potential, and lower mitochondrial transmembrane potential in Hepg2 cells after a 24-hour exposure to chlorpyrifos. In another in vitro study (31) chlorpyrifos-ethyl induced lipid peroxidation and disturbed the activity of antioxidant enzymes in erythrocytes, which suggests an involvement of ROS in the toxic effects of OP pesticides.…”

Section: Organophosphatesmentioning

confidence: 99%

Redox imbalance caused by pesticides: a review of OPENTOX-related research

Čermak

Pavičić

Želježić

2018

Archives of Industrial Hygiene and Toxicology

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Pesticides are a highly diverse group of compounds and the most important chemical stressors in the environment. Mechanisms that could explain pesticide toxicity are constantly being studied and their interactions at the cellular level are often observed in well-controlled in vitro studies. Several pesticide groups have been found to impair the redox balance in the cell, but the mechanisms leading to oxidative stress for certain pesticides are only partly understood. As our scientific project "Organic pollutants in environment - markers and biomarkers of toxicity (OPENTOX)" is dedicated to studying toxic effects of selected insecticides and herbicides, this review is focused on reporting the knowledge regarding oxidative stress-related phenomena at the cellular level. We wanted to single out the most important facts relevant to the evaluation of our own findings from studies conducted on in vitro cell models.

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Cytotoxicity of chlorpyrifos to human liver hepatocellular carcinoma cells: effects on mitochondrial membrane potential and intracellular free Ca²⁺

Cited by 15 publications

References 30 publications

Aerobic exercise and eugenol supplementation ameliorated liver injury induced by chlorpyrifos via modulation acetylcholinesterase activation and antioxidant defense

Aerobic exercise and eugenol supplementation ameliorated liver injury induced by chlorpyrifos via modulation acetylcholinesterase activation and antioxidant defense

Chlorpyrifos induces genotoxic effects in human leukocytes in <i>vitro</i> at low concentrations

Redox imbalance caused by pesticides: a review of OPENTOX-related research

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Cytotoxicity of chlorpyrifos to human liver hepatocellular carcinoma cells: effects on mitochondrial membrane potential and intracellular free Ca2+

Cited by 15 publications

References 30 publications

Aerobic exercise and eugenol supplementation ameliorated liver injury induced by chlorpyrifos via modulation acetylcholinesterase activation and antioxidant defense

Aerobic exercise and eugenol supplementation ameliorated liver injury induced by chlorpyrifos via modulation acetylcholinesterase activation and antioxidant defense

Chlorpyrifos induces genotoxic effects in human leukocytes in <i>vitro</i> at low concentrations

Redox imbalance caused by pesticides: a review of OPENTOX-related research

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Cytotoxicity of chlorpyrifos to human liver hepatocellular carcinoma cells: effects on mitochondrial membrane potential and intracellular free Ca²⁺