Chlorpyrifos induces genotoxic effects in human leukocytes in &lt;i&gt;vitro&lt;/i&gt; at low concentrations

Serpa, Élvio Adílio; Schmitt, Elisandra Gomes; Zuravski, Luísa; Machado, Michel Mansur; Oliveira, Luís Flávio Souza de

doi:10.4025/actascihealthsci.v41i1.44291

Cited by 6 publications

(3 citation statements)

References 22 publications

(26 reference statements)

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“…The results obtained by Serpa et al [116] confirm the genotoxic potential of CPF. They demonstrated the genotoxic effect of CPF in vitro in human leukocytes at a concentration of 35 µg/mL by causing the following alterations: micronuclei, numerical chromosomal abnormalities, and apoptotic cells [117].…”

Section: Genotoxicity Of Cpfmentioning

confidence: 60%

Chlorpyrifos Occurrence and Toxicological Risk Assessment: A Review

Wołejko

Łozowicka

Jabłońska-Trypuć

et al. 2022

IJERPH

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Chlorpyrifos (CPF) was the most frequently used pesticide in food production in the European Union (EU) until 2020. Unfortunately, this compound is still being applied in other parts of the world. National monitoring of pesticides conducted in various countries indicates the presence of CPF in soil, food, and water, which may have toxic effects on consumers, farmers, and animal health. In addition, CPF may influence changes in the population of fungi, bacteria, and actinomycete in soil and can inhibit nitrogen mineralization. The mechanisms of CPF activity are based on the inhibition of acetylcholinesterase (AChE) activity. This compound also exhibits reproductive toxicity, neurotoxicity, and genotoxicity. The problem seems to be the discrepancy between the actual observations and the final conclusions drawn for the substance’s approval in reports presenting the toxic impact of CPF on human health. Therefore, this influence is still a current and important issue that requires continuous monitoring despite its withdrawal from the market in the EU. This review traces the scientific reports describing the effects of CPF resulting in changes occurring in both the environment and at the cellular and tissue level in humans and animals. It also provides an insight into the hazards and risks to human health in food consumer products in which CPF has been detected.

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Section: Genotoxicity Of Cpfmentioning

confidence: 60%

Chlorpyrifos Occurrence and Toxicological Risk Assessment: A Review

Wołejko

Łozowicka

Jabłońska-Trypuć

et al. 2022

IJERPH

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“…For instance, and with regard to the pesticides used most frequently by the exposed individuals included in our study, associations between mancozeb exposure with a significant increase in the frequencies of structural chromosomal alterations and genotoxic damage were reported ( Jablonicka et al, 1989 ; Srivastava et al, 2012 ). In addition, in vitro studies in human lymphocytes demonstrated associations between exposure to paraquat and the production of isochromatic breaks ( Jovtchev et al, 2010 ), as well as between high concentrations of chlorpyrifos with an increase in the number of numerical chromosomal alterations ( Serpa et al, 2019 ), and between sublethal concentrations of profenofos with the induction of chromatid breaks and gaps ( Prabhavathy Das et al, 2006 ). In the same way, in vivo cytogenetic analysis demonstrated the induction of chromosomal alterations and micronucleus (MN) formation in mouse bone marrow cells exposed to furadan ( Chauhan et al, 2000 ).…”

Section: Discussionmentioning

confidence: 99%

Profile of Chromosomal Alterations, Chromosomal Instability and Clonal Heterogeneity in Colombian Farmers Exposed to Pesticides

et al. 2022

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Pesticides are a group of environmental pollutants widely used in agriculture to protect crops, and their indiscriminate use has led to a growing public awareness about the health hazards associated with exposure to these substances. In fact, exposure to pesticides has been associated with an increased risk of developing diseases, including cancer. In a study previously published by us, we observed the induction of specific chromosomal alterations and, in general, the deleterious effect of pesticides on the chromosomes of five individuals exposed to pesticides. Considering the importance of our previous findings and their implications in the identification of cytogenetic biomarkers for the monitoring of exposed populations, we decided to conduct a new study with a greater number of individuals exposed to pesticides. Considering the above, the aim of this study was to evaluate the type and frequency of chromosomal alterations, chromosomal variants, the level of chromosomal instability and the clonal heterogeneity in a group of thirty-four farmers occupationally exposed to pesticides in the town of Simijacá, Colombia, and in a control group of thirty-four unexposed individuals, by using Banding Cytogenetics and Molecular Cytogenetics (Fluorescence in situ hybridization). Our results showed that farmers exposed to pesticides had significantly increased frequencies of chromosomal alterations, chromosomal variants, chromosomal instability and clonal heterogeneity when compared with controls. Our results confirm the results previously reported by us, and indicate that occupational exposure to pesticides induces not only chromosomal instability but also clonal heterogeneity in the somatic cells of people exposed to pesticides. This study constitutes, to our knowledge, the first study that reports clonal heterogeneity associated with occupational exposure to pesticides. Chromosomal instability and clonal heterogeneity, in addition to reflecting the instability of the system, could predispose cells to acquire additional instability and, therefore, to an increased risk of developing diseases.

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“…Raj and Kumar, 2022;Serpa et al, 2019; USEPA, 2022) and also declared carcinogenic substances by the International Agency for Research on Cancer (IARC). Notably, Nigerian environmental legislation does not specify maximum permissible limits for pesticides in river water.…”

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confidence: 99%

Exploring the Relationship Between Land Use and Pesticide Contamination in a Tropical Rural Catchment: A Case Study of River Chanchaga

Ikayaja,

Tshimanga,

Ndatimana

et al. 2024

Preprint

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Freshwater ecosystems are vulnerable to various land uses, resulting in concern for aquatic organisms and humans. Understanding the occurrence of pesticide contamination is necessary to safeguard aquatic biodiversity and human health. Against this background, our study aimed to assess the effect of various land uses on pesticide contamination by evaluating three selected sites according to land use class: a control site (CON), an agricultural area (S1), and an urban area (S2). Various classes of land use around the catchment were determined using ArcGIS 10.8 GIS software, while analysis of pesticide residues was carried out using the liquid-liquid extraction method, followed by gas chromatography-mass spectrometry (GC/MS). A total of 10 herbicides and 12 insecticides were recorded in each sampled sub-catchment, with a statistically significant difference across the sampled sub-catchment. Sampled sites located in agricultural and urban areas recorded higher concentrations of pesticide residues compared to sites located in natural areas, with less anthropogenic influence, reflecting differences in pesticide usage. Redundancy analysis (RDA) revealed crops and urbanized land use were the main sources of pesticide contamination in the water, as pesticides are predominantly used on farms and in various households. Their presence in the water may have potentially detrimental effects on aquatic biodiversity because pesticides have been associated with various health issues. It was evident that all sampled sub-catchments had pesticide concentrations exceeding the WHO permissible limit for human consumption. Implementing alternative methods for managing pests and weeds without relying on pesticides is crucial for sustainable agriculture and environmental preservation.

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Chlorpyrifos induces genotoxic effects in human leukocytes in <i>vitro</i> at low concentrations

Cited by 6 publications

References 22 publications

Chlorpyrifos Occurrence and Toxicological Risk Assessment: A Review

Chlorpyrifos Occurrence and Toxicological Risk Assessment: A Review

Profile of Chromosomal Alterations, Chromosomal Instability and Clonal Heterogeneity in Colombian Farmers Exposed to Pesticides

Exploring the Relationship Between Land Use and Pesticide Contamination in a Tropical Rural Catchment: A Case Study of River Chanchaga

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