Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Kim, Ryan Jin Young; Shin, Joo Hee

doi:10.4012/dmj.2013-171

Cited by 28 publications

(35 citation statements)

References 25 publications

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“…Sealers may be classified according to their main chemical content, which usually includes calcium hydroxide (CaOH) or an epoxy resin [1]. Because of the physical limitations of core materials, sealers play an important role in root canal obturation, as they fill all voids and spaces within the tooth [2].…”

Section: Introductionmentioning

confidence: 99%

Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS

Şımşek

Akinci

Alan

et al. 2017

Biotechnology & Biotechnological Equipment

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Following root canal treatment, sealers may contact periapical tissue. The purpose of this study was to evaluate the systemic toxic effects of epoxy resin-based sealers (AH Plus and Obtuseal). Inductively coupled plasma-mass spectrometry (ICP-MS) was used to measure levels of trace elements (beryllium, magnesium, aluminium, calcium, chromium, arsenic and lead) in the brain, kidney and liver of rats. Twenty sterilized polyethylene tubes were then filled with AH Plus and Obtuseal and implanted into the dorsal subcutaneous tissue of 10 rats; three unoperated animals were used as a control group. After 45 days, the rats were sacrificed by cervical dislocation following anaesthesia, and brains, kidneys and livers were removed from all experimental animals. ICP-MS analysis was used to determine levels of trace elements. Data were analysed using KruskalWallis and Connover post hoc tests. No significant differences were found in aluminium and calcium levels, but brains, kidneys and livers showed significantly higher amounts of magnesium and chromium than the corresponding controls. In the kidney and liver samples, arsenic levels were found to be higher than in the control group. Lead was detected at higher levels only in liver samples from the AH Plus group. Beryllium was not detected in any organ. It was concluded that AH Plus and Obtuseal release minimal quantities of trace elements when in contact with subcutaneous tissue, and further studies are needed to understand the systemic effects of these materials.

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Section: Introductionmentioning

confidence: 99%

Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS

Şımşek

Akinci

Alan

et al. 2017

Biotechnology & Biotechnological Equipment

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“…To fulfill these requirements Mineral Trioxide Aggregate (MTA) was introduced by Torabinejad and White in 1990s 3) . Since its introduction, MTA has gained acceptance in variety of endodontic treatments for example root end fillings, repair of lateral perforations, direct pulp capping, pulpotomy and apexification 2,4) . MTA is marketed in two forms i.e.…”

Section: Introductionmentioning

confidence: 99%

Chemical and morphological characteristics of mineral trioxide aggregate and Portland cements

et al. 2016

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The purpose of this study was to investigate the chemical composition and particle morphology of white mineral trioxide aggregate (WMTA) and two white Portland cements (CEM 1 and CEM 2). Compositional analysis was performed by energy dispersive X-ray spectroscopy, X-ray fluorescence spectrometry and X-ray diffraction whereas, morphological characteristics were analyzed by scanning electron microscope and Laser scattering particle size distribution analyzer. The elemental composition of WMTA, CEM 1 and CEM 2 were similar except for the presence of higher amounts of bismuth in WMTA. Calcium oxide and silicon oxide constitute the major portion of the three materials whereas, tricalcium silicate was detected as the major mineral phase. The particle size distribution and morphology of WMTA was finer compared to CEM 1 and CEM 2. The three tested materials had relatively similar chemical composition and irregular particle morphologies.

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“…Devido às excelentes propriedades físico-químicas e biológicas do MTA, novos materiais compostos pelo agregado trióxido mineral surgiram recentemente. [13][14][15][16] Contudo as diversas composições diferentes e as proporções distintas de MTA nas formulações promovem resultados de citotoxicidade variados a estes cimentos com o mesmo componente base. 14,17 O MTA Fillapex foi o material que apresentou a maior citotoxicidade em valores absolutos em todas as diluições e entre todos os cimentos endodônticos testados.…”

Section: Discussionunclassified

Avaliação da citotoxicidade de dois cimentos à base de MTA: um estudo in vitro

Marins

Ronconi

Saavedra

et al. 2017

Rev. Bras. Odontol.

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• Os autores declaram que não há conflito de interesse. ResumoObjetivo: avaliar o potencial citotóxico de dois cimentos endodônticos à base de MTA (Endoseal e MTA Fillapex), em culturas de fibroblastos humanos, comparando-os com o cimento de referência à base de resina (AH Plus). Material e Métodos: os materiais obturadores foram preparados e eluídos em meio de cultura celular durante 24 horas à 37°C em estufa de CO 2 . Foram feitas quatro diluições distintas destes meios nas concentrações 1:1, 1:2, 1:4, 1:8 e expostos à cultura celular de fibroblastos humanos da linhagem MRC-5 por 24 horas. A citotoxicidade destes meios de cultura foi avaliada utilizando o ensaio MTT. Os resultados foram transformados em porcentagens de células viáveis com relação ao grupo controle negativo e analisados estatisticamente a partir da Análise de Variância (ANOVA) e pelo teste de Tukey utilizando o software SPSS 15.0 (SPSS Inc, Chicago,IL) (P < 0,05). Resultados: na diluição 1:1 não houve diferença estatística entre os cimentos testados (P > 0,05). Na diluição 1:2 o AH Plus apresentou menor toxicidade quando comparado aos demais cimentos testados (P < 0,05). Cytotoxicity evaluation of two MTA-based root canal sealers: an in vitro study IntroduçãoA obturação do sistema de canais radiculares tem como objetivo o total preenchimento do sistema de canais radiculares recém-descontaminados, a fim de impedir a microinfiltração bacteriana do meio oral, dos tecidos apicais e periapicais para o interior dos mesmos.1,2 Convencionalmente, a obturação é composta pela utilização de cones de guta-percha em combinação com algum cimento endodôntico. A principal função do cimento é preencher espaços existentes entre a guta-percha e as paredes do canal radicular. 2Os cimentos endodônticos podem ser encontrados comercialmente em diversas formulações. Contudo, independente de seus componentes, tais materiais devem contemplar algumas propriedades importantes, tais como a sua boa tolerância pelos tecidos periapicais, uma vez que os cimentos endodônticos podem permanecer em contato íntimo com os tecidos periapicais por um período prolongado, devido a sua possível extrusão através do ápice ou por subprodutos da sua degradação que podem percolar através de canais laterais, acessórios ou forame apical, alcançando assim, os tecidos circundantes. [3][4][5] O Agregado Trióxido Mineral (MTA) é um material que possui propriedades biológicas e fisico-químicas adequadas para o seu uso na Endodontia, 6,7 sendo considerado um material próxi-mo do ideal em termos de biocompatibilidade.6-8 Com base nas características biológicas favoráveis do MTA e buscando melhorar as desvantagens físicas da formulação do MTA convencional, novos cimentos obturadores a base de MTA foram lançados no mercado odontológico. O EndoSeal (Maruchi, Wonju, Coréia do Sul) e o MTA Fillapex (Angelus,Londrina, PR, Brasil) são exemplos de cimentos endodônticos à base de MTA. O MTA Filapex é um cimento endodôntico composto de duas pastas. Após sua manipulação, este cimento consiste em MTA, resina sal...

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Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Cited by 28 publications

References 25 publications

Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS

Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS

Chemical and morphological characteristics of mineral trioxide aggregate and Portland cements

Avaliação da citotoxicidade de dois cimentos à base de MTA: um estudo in vitro

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