Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS

Şımşek, Neslihan; Akinci, Levent; Alan, Hilal; Geçör, Orhan; Özan, Ülkü

doi:10.1080/13102818.2017.1282327

“…Inductive coupled plasma mass spectrometry (ICP-MS) is a robust technique for molecular analysis of element and physiochemical compound with separation techniques that makes it suitable for detecting elements in pharmaceutical research and for specific investigation of element present in molecule [44]. ICP-MS is a multi-element system techniques in the analysis of biological fluids with greater sensitivity and selectivity when compared to inductive coupled plasma-optical emission spectrometry (ICP-OES) and graphite furnace atomic absorption spectrometry (GF-AAS) [45].…”

Section: Discussionmentioning

confidence: 99%

“…Concentration of trace elements beyond physiological limits in organs can be toxic in both animals and humans. Likewise, concentration of heavy metals such as Pb in the biological system are known to be toxic thus, affecting biochemical reactions [45]. Further, bones remain a vital site for Pb accumulation after exposure, although circulating Pb in the blood can be distributed to various vital organs such as the brain, kidney and liver [46,47].…”

Section: Discussionmentioning

confidence: 99%

Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Inhibition of Oxidative Stress and Chelating Activity

Abubakar¹,

Mailafiya²,

Danmaigoro³

et al. 2019

Preprint

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Lead (Pb) is a toxic environmental heavy metal that induces serious clinical defect on all organs with the nervous system being the primary target. Curcumin is the main active constituent of turmeric rhizome (Curcuma longa) with strong antioxidant and anti-inflammatory properties. This study is aimed at evaluating the therapeutic potentials of curcumin on Pb-induced neurotoxicity. Thirty six male Sprague Dawley rats were randomly assigned into five (5) groups with 12 rats in the control (normal saline) and 6 rats for the lead treated group (LTG) (50 mg/kg lead acetate for 4 weeks), recovery group (RC) (50 mg/kg lead acetate for 4 weeks), treatment group 1 (Cur100) (50 mg/kg lead acetate for 4 weeks, followed by 100 mg/kg curcumin for 4 weeks) and treatment group 2 (Cur200) (50 mg/kg lead acetate for 4 weeks, followed by 200 mg/kg curcumin for 4 weeks) groups each. All experimental groups received the oral treatments through orogastric-tube on alternate days. Motor function was assessed using horizontal bar method while Pb concentration in the cerebellum of the rats were evaluated using ICP-MS techniques. Pb-administered rats showed significant decrease in motor scores, SOD activity with increase MDA levels and Pb concentration in their cerebellum with marked alterations in the histological architecture of the cerebellar cortex layers. However, treatment with curcumin improved the motor score, reduced Pb concentration in the cerebellum and ameliorates the markers of oxidative stress as well as restored the histological architecture of the cerebellum. The results this in study suggested that curcumin attenuates Pb-induced neurotoxicity via inhibition of oxidative stress and chelating activity.

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“…Inductive coupled plasma mass spectrometry (ICP-MS) is a robust technique for molecular analysis of element and physiochemical compound with separation techniques that makes it suitable for detecting elements in pharmaceutical research and for specific investigation of element present in molecule [50]. ICP-MS is a multi-element system techniques in the analysis of biological fluids with greater sensitivity and selectivity when compared to inductive coupled plasma-optical emission spectrometry (ICP-OES) and graphite furnace atomic absorption spectrometry (GF-AAS) [24].…”

Section: Discussionmentioning

confidence: 99%

“…Concentration of trace elements beyond physiological limits in organs can be toxic in both animals and humans. Likewise, concentration of heavy metals such as Pb in the biological system are known to be toxic thus, affecting biochemical reactions [24]. Further, bones remain a vital site for Pb accumulation after exposure, although circulating Pb in the blood can be distributed to various vital organs such as the brain, kidney and liver [51,52].…”

Section: Discussionmentioning

confidence: 99%

Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Inhibition of Oxidative Stress and Chelating Activity

Abubakar¹,

Mm²,

Danmaigoro³

et al. 2019

Preprint

View full text Add to dashboard Cite

Lead (Pb) is a toxic environmental heavy metal that induces serious clinical defect on all organs with the nervous system being the primary target. Curcumin is the main active constituent of turmeric rhizome (Curcuma longa) with strong antioxidant and anti-inflammatory properties. This study is aimed at evaluating the therapeutic potentials of curcumin on Pb-induced neurotoxicity. Thirty six male Sprague Dawley rats were randomly assigned into five (5) groups with 12 rats in the control (normal saline) and 6 rats for the lead treated group (LTG) (50 mg/kg lead acetate for 4 weeks), recovery group (RC) (50 mg/kg lead acetate for 4 weeks), treatment group 1 (Cur100) (50 mg/kg lead acetate for 4 weeks, followed by 100 mg/kg curcumin for 4 weeks) and treatment group 2 (Cur200) (50 mg/kg lead acetate for 4 weeks, followed by 200 mg/kg curcumin for 4 weeks) groups each. All experimental groups received the oral treatments through orogastric-tube on alternate days. Motor function was assessed using horizontal bar method while Pb concentration in the cerebellum of the rats were evaluated using ICP-MS techniques. Pb-administered rats showed significant decrease in motor scores, SOD activity with increase MDA levels and Pb concentration in their cerebellum with marked alterations in the histological architecture of the cerebellar cortex layers. However, treatment with curcumin improved the motor score, reduced Pb concentration in the cerebellum and ameliorates the markers of oxidative stress as well as restored the histological architecture of the cerebellum. The results this in study suggested that curcumin attenuates Pb-induced neurotoxicity via inhibition of oxidative stress and chelating activity.

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“…Median arsenic concentrations (in dry weight) in the organs of healthy people range from 0.012 µg/g in the brain to 0.46 µg/g in hair, while concentrations in the organs of healthy people from Japan (who generally consume relatively high amounts of seafood) range from 0.02 µg/g wet weight to 0.89 µg/g in nails (24). In fatal cases of arsenic poisoning, the concentrations of arsenic in tissues changed and exceeded the above values, showing widespread distribution of arsenic in all organs, the highest levels (in decreasing order) were observed in liver, kidney, brain, lungs, heart, pancreas, spleen, and muscles (25). However, comparative studies of trace elements and heavy metals in cardiovascular tissues are yet difficult due to the lack of -normal" values and arsenic speciation in human tissues is scarce (26,27).…”

Section: Issn (Online)mentioning

confidence: 99%

Environmental Contamination of Arsenic and its Toxicological Impact in Cardiovascular Tissues of Patients with Coronary Heart Disease

2017

IJSR

0

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Background: The concentration levels of As in the Chilean II Region of Antofagasta produces non cancer health outcomes such as cardiovascular diseases and in last term heart attack. On this study, the determination of total As content and main inorganic and organoarsenic species found in three heart tissues (auricle, mammary artery and fat) and the saphenous vein of people living in the Chilean II Region, suffering coronary thrombosis has been carried out. Comparison with similar tissues of patients from other noncontaminated areas has been undertaken. Methods: Total As determination has been performed by HG-AAS and ICP-MS. For As species have been used the tandem HPLC-ICP-MS using the Hamilton PRP X100 anion column and methanol:water (1:1) extraction. Results: The auricle and in less extend the saphenous vein support the higher As concentration (mean values of 7.7 and 2.5 µg g -1 , respectively), being As(III) the predominant species. Methylation towards MMA and DMA is not a favoured mechanism. Conclusions: The presence of high total As and high As(III) species content in the auricle and saphenous vein of more contaminated people, the damage found in the saphenous vein tissue and the global characteristics of the people under study in which the As stigmas are present in all of them, suggests that As could be involved in the cardiovascular diseases.

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Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS

Cited by 7 publications

References 34 publications

Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Inhibition of Oxidative Stress and Chelating Activity

Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Inhibition of Oxidative Stress and Chelating Activity

Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Inhibition of Oxidative Stress and Chelating Activity

Environmental Contamination of Arsenic and its Toxicological Impact in Cardiovascular Tissues of Patients with Coronary Heart Disease

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