Cytotoxicity difference of 316L stainless steel and titanium reconstruction plate

Sumarta, Ni Putu Mira; Danudiningrat, Coen Pramono; Rachmat, Ester Arijani; Soesilawati, Pratiwi

doi:10.20473/j.djmkg.v44.i1.p7-11

Cited by 7 publications

(9 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Research involving cytotoxic tests of variable concentrations of Aloe vera against fibroblast cell culture shows that a concentration of Aloe vera 90% is nontoxic against fibroblast cell culture. 7 The research result states that Aloe vera promotes wound healing by improving cell regeneration and increasing the number of phagocytic cells, that it has a hypoglycemic effect, is antiinflammatory, anti-bacterial, anti-viral, anti-fungal, and is an immunomodulator. 8,9 Aloe vera contains mannose-6-phospates, chromones, β-sitosterol, and bradykinase as an anti-inflammation agent.…”

Section: Introductionmentioning

confidence: 99%

The possibility of polymorphonuclear leukocyte activation in dental socket healing by freeze-dried Aloe vera induction

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background: Research has proved that Aloe vera has anti-inflammatory, anti-bacterial, and immunomodulator properties that can accelerate the healing process. These properties could be beneficial in the tooth extraction wound-healing process. Neutrophils are the first inflammatory cells to invade and are the predominant cell markers in the wound for 24 hours after injury. Polymorphonuclear leukocytes (PMN) provide initial protection against micro-organisms, as they engulf and digest foreign bodies. Purpose: The aim of this study was to prove the effect of Aloe vera 90% gel application on the PMN count in the healing process of Cavia cobaya following tooth extraction. Methods: This laboratory experimental research was done using Post-Test Only Control Group Design. The lower incisive of twelve male Cavia cobayas were extracted then divided into four groups. Group P1 and P3 had 90% Aloe vera gel applied into the socket and sutured; group K1 and K3, received no 90% Aloe vera gel application and acted as controls. Samples from group P1 and K1 were terminated and had their mandibula taken one day after tooth extraction, and those from group P3 and K3 were terminated after three days. Tissues were then processed into histology slides, and PMN cells were counted. Results: There were significant differences in the PMN count between groups K1, P1, and K3 compared with P3 (p < 0.05). Higher PMN count was shown on the group containing Aloe vera 90% gel compared with control group, both on day one and three after tooth extraction. Conclusion: Aloe vera 90% gel can increase the PMN count in the inflammation phase of the tooth extraction wound-healing process.

show abstract

Section: Introductionmentioning

confidence: 99%

The possibility of polymorphonuclear leukocyte activation in dental socket healing by freeze-dried Aloe vera induction

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The particle concentration ranging from 0.5 to 50mg/L did not play a significant role. [ 13 ] Cell viability study by Lin et al .…”

Section: Discussionmentioning

confidence: 99%

“…Viable cell percentage was calculated using the IC 50 formula. [ 13 ] The amount of IL-6 on culture medium was quantified by an immunoassay kit (ELISA; Koma Biotech, Yeongdeungpo-gu, Seoul, Korea).…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expression

Anwar¹,

Santoso²,

Saputra

et al. 2018

J Pharm Bioall Sci

View full text Add to dashboard Cite

Introduction:Ultrahigh-molecular-weight polyethylene (UHMWPE) is a thermoplastic polymer useful in biomaterial applications, especially in orthopedic field. Yet, little is known concerning its initial effect on human bone marrow stem cells (hBMSCs) after implantation.Materials and Methods:A cytotoxicity analysis was performed with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium assay after 24, 48, and 72h of incubation of hBMSC culture. Expression of interleukin-6 (IL-6) was measured using enzyme-linked immunosorbent assay. Cell viability was measured with Inhibitory concentration 50% (IC50) formula.Results:All treatment groups showed a cell viability of >50% ranging from 78% to >100%. Lower expression of IL-6 of hBMSC compared to control group was found in 48h of incubation period.Conclusion:hBMSC showed high cell viability after initial contact with UHMWPE material. Modulation of IL-6 expression was present at the initial stage as a response to foreign material.

show abstract

“…Optical density data were tabulated and analyzed using the t -test (SPSS v17, Chicago, USA) at a significance of 95%. Percentage of viable hBMC were calculated using the IC 50 formula: 5 …”

Section: Methodsmentioning

confidence: 99%

Human Bone Marrow-Derived Mesenchymal Cell Reactions to 316L Stainless Steel: An in Vitro Study on Cell Viability and Interleukin-6 Expression

Anwar

Santoso²,

Saputra

et al. 2017

Adv Pharm Bull

View full text Add to dashboard Cite

Purpose: Human bone marrow-derived mesenchymal cell (hBMC) reactions to 316L stainless steel (316L-SS) have never been evaluated. The objective of this study was to assess cell viability and interleukin-6 expression of hBMC cultures upon treatment with a 316L-SS implant. Methods: A cytotoxicity analysis was conducted with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium (MTT) assay after a period of 24, 48 and 72 hours of incubation. Expression of interleukin-6 was measured using enzyme-linked immunosorbent assay (ELISA). Results: Cell viability measurement was performed via IC50 formula. All treatment group showed a > 50 % cell viability with a range of 56,5 - 96,9 % at 24 hours, 51,8-77,3% at 48 hours and 70,1- 120 % at 72 hours. Interleukin-6 expression was downregulated subsequent to treatment with 316L-SS compared to the control group. Conclusion: We found that 316L-SS did not exhibit toxicity towards hBMC culture.

show abstract

Cytotoxicity difference of 316L stainless steel and titanium reconstruction plate

Cited by 7 publications

References 10 publications

The possibility of polymorphonuclear leukocyte activation in dental socket healing by freeze-dried Aloe vera induction

The possibility of polymorphonuclear leukocyte activation in dental socket healing by freeze-dried Aloe vera induction

Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expression

Human Bone Marrow-Derived Mesenchymal Cell Reactions to 316L Stainless Steel: An in Vitro Study on Cell Viability and Interleukin-6 Expression

Contact Info

Product

Resources

About