Cytotoxicity and gene expression profiling of polyhexamethylene guanidine hydrochloride in human alveolar A549 cells

Jung, Hwa Jin; Zerin, Tamanna; Podder, Biswajit; Song, Ho‐Yeon; Kim, Yong-Sik

doi:10.1016/j.tiv.2014.02.004

Cited by 49 publications

(41 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, changes in gene expression relevant to the progression of cell death included genes related to apoptosis, autophagy, fibrosis, and cell cycle. The generation of ROS and the reduction of cellular defenserelated antioxidant genes might be one of the reasons for PHMG-induced cytoxicity 35) .…”

Section: Discussionmentioning

confidence: 99%

Physical and biological properties of a novel root canal sealer modified by polyhexamethylene guanidine

et al. 2020

View full text Add to dashboard Cite

The key of the root canal therapy is to eliminate the microorganism infection, fill the root canal tightly and reduce the stimulation to the periapical tissues. However, it is quite difficult to meet all the conditions due to the defect of the material. Here we develop a novel root canal sealer (MZOE), in which zinc oxide eugenol (ZOE) were fabricated with polyhexamethylene guanidine (PHMG), and the PHMG's concentration is 0.8, 1.0, 1.2 and 1.4%. Our investigation tested its physical properties, antibacterial effect to E. faecalis, C. albicans, E. coli, S. aureus and cytotoxicity to human periodontal ligament fibroblasts (HPDLFs). The physical properties of the MZOE conformed to the ISO 6876:2001, and its antibacterial effect was stronger than ZOE (p<0.05), the RGR of HPDLFs was tested between 1 to 24%, belonging to moderate cytotoxicity. It was suggested that MZOE had good physical properties, high antibacterial effect, and moderate cytotoxicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Physical and biological properties of a novel root canal sealer modified by polyhexamethylene guanidine

et al. 2020

View full text Add to dashboard Cite

show abstract

“… 24 The production of oxidative stress and changes in the expression of oxidative stress-related genes after exposure to toxic chemicals in HD, such as PHMG/PGH and CMIT/MIT, might be a common pathway in the development of HDLIs. 12 25 26 …”

Section: Discussionmentioning

confidence: 99%

Two Cases of Chloromethylisothiazolinone and Methylisothiazolinone-associated Toxic Lung Injury

et al. 2018

View full text Add to dashboard Cite

Previous animal studies have not conclusively determined the association between exposure to humidifier disinfectants (HDs) containing 5-chloro-2-methyl-4-isothiazolin-3-one (CMIT) and/or 2-methyl-4-isothiazolin-3-one (MIT) and development of HD-associated lung injuries. Nonetheless, patients exposed to HDs containing only CMIT and/or MIT showed clinically similar lung injuries to those exposed to HDs containing polyhexamethylene guanidine (PHMG) or oligo (2-[2-ethoxy]ethoxyethyl) guanidinium chloride (PGH). Here, we report twin sisters with lung injuries associated with exposure to CMIT/MIT-containing HDs. At 6 months of age, a younger twin sister presented with the 3-day history of cough, sputum, and respiratory difficulty. Chest radiography revealed multiple patchy consolidation and ground-glass opacities with pneumothorax and pneumomediastinum. Thoracostomy was performed due to pneumothorax at admission and she was discharged at 11 days of hospitalization. At 5 years of age, multiple tiny nodules and faint centrilobular ground-glass opacities were observed with the small pneumatocele. The elder sister visited a tertiary hospital due to dyspnea at 12 months of age. Chest radiography showed consolidation, pneumomediastinum, and pulmonary interstitial emphysema. There was no response to the administration of immunosuppressant drugs and antifibrotic agents. At 5 years of age, chest CT revealed ground-glass opacity and multiple tiny centrilobular ground-glass opacities nodules in both lungs with exercise intolerance.

show abstract

“…PHMG has been reported to cause cytotoxicity through the production of intracellular reactive oxygen species through alterations in gene expression. 17,[23][24][25] Although most published studies have identified the health effects of PHMG and PGH, studies on health effects of inhalational exposure to CMIT and MIT are lacking. In real clinical situations in humans, HD-ILDs had been occurred after exposure to CMIT and MIT, which showed similar patterns of pulmonary inflammation and fibrosis as those associated with exposure to PHMG and PGH.…”

Section: Health Effects Of Toxic Chemicals In Hdsmentioning

confidence: 99%

The past, present, and future of humidifier disinfectant-associated interstitial lung diseases in children

Lee

Hong

2020

Clin Exp Pediatr

View full text Add to dashboard Cite

Exposure to environmental factors can cause interstitial lung diseases (ILDs); however, such types of ILDs are rare. From 2007 to 2011, an ILD epidemic occurred in South Korea owing to inhalational exposure to toxic chemicals in humidifier disinfectants (HDs). HD-associated ILDs (HD-ILDs) are characterized by rapidly progressing respiratory failure with pulmonary fibrosis and a high mortality rate of 43.8%−58.0%. Although 18.1%−31.1% of the general population used HDs, only a small proportion of HD users were diagnosed with HD-ILDs. This finding suggests that investigation of the pathophysiologies underlying HD-ILDs is needed in addition to the identification of susceptibility to HD-ILDs. Further, there have been several concerns regarding the diverse health effects of exposure to toxic chemicals in HDs, including those that have not been identified, and long-term prognoses in terms of pulmonary function and residual pulmonary lesions observed on follow-up chest images. In this review, we summarize the clinical features, pathologic findings, and changes in radiologic findings over time in patients with HD-ILDs and the results of previous experimental research on the mechanisms underlying the effects of toxic chemicals in HDs. Studies are currently underway to identify the pathophysiologies of HD-ILDs and possible health effects of exposure to HDs along with the development of targeted therapeutic strategies. The experience of identification of HD-ILDs has encouraged stricter control of safe chemicals in everyday life.

show abstract

Cytotoxicity and gene expression profiling of polyhexamethylene guanidine hydrochloride in human alveolar A549 cells

Cited by 49 publications

References 21 publications

Physical and biological properties of a novel root canal sealer modified by polyhexamethylene guanidine

Physical and biological properties of a novel root canal sealer modified by polyhexamethylene guanidine

Two Cases of Chloromethylisothiazolinone and Methylisothiazolinone-associated Toxic Lung Injury

The past, present, and future of humidifier disinfectant-associated interstitial lung diseases in children

Contact Info

Product

Resources

About