Physical and biological properties of a novel root canal sealer modified by polyhexamethylene guanidine

Abstract

The key of the root canal therapy is to eliminate the microorganism infection, fill the root canal tightly and reduce the stimulation to the periapical tissues. However, it is quite difficult to meet all the conditions due to the defect of the material. Here we develop a novel root canal sealer (MZOE), in which zinc oxide eugenol (ZOE) were fabricated with polyhexamethylene guanidine (PHMG), and the PHMG's concentration is 0.8, 1.0, 1.2 and 1.4%. Our investigation tested its physical properties, antibacterial effect to E. faecalis, C. albicans, E. coli, S. aureus and cytotoxicity to human periodontal ligament fibroblasts (HPDLFs). The physical properties of the MZOE conformed to the ISO 6876:2001, and its antibacterial effect was stronger than ZOE (p<0.05), the RGR of HPDLFs was tested between 1 to 24%, belonging to moderate cytotoxicity. It was suggested that MZOE had good physical properties, high antibacterial effect, and moderate cytotoxicity.

Irrigation in Endodontics: Polyhexanide Is a Promising Antibacterial Polymer in Root Canal Treatment

“…The largest growth retardation zone after incubation at 37 °C was demonstrated for E. coli —46.24 mm—which the authors associated with the structure of the cell wall of Gram-negative bacteria. Smaller growth retardation zones were demonstrated for Gram-positive microorganisms— E. Faecalis and S. Aureus —at 41.52 mm and 42.18 mm, respectively [ 62 ]. Perhaps polyhexanide binds worse to the components of the Gram-positive bacteria cell wall, such as teichoic and lipoteichoic acids, which can explain the smaller diameter of the “clean zones”.…”