Cytotoxicity and antibacterial property of new resin-based sealer

Park, Soyoung; Lee, Woo-Cheol; Lim, Sung-Sam

doi:10.5395/jkacd.2003.28.2.162

Cited by 10 publications

(6 citation statements)

References 12 publications

(13 reference statements)

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“…In accordance with the current finding, the epoxy-resin-based sealer previously displayed a slightly neutral pH throughout the experimental period [12,23], which may attribute to its low solubility [24]. Despite Adseal displaying a neutral pH, its previously determined high antibacterial effect against black pigmented bacteria was due to its calcium phosphate content [25]. This premise was reinforced by Faria-Júnior et al [14] who concluded that the neutral pH and low solubility of sealer would eliminate its antibacterial activity.…”

Section: Discussionsupporting

confidence: 88%

Solubility, pH Changes and Releasing Elements of Different Bioceramic and Mineral Trioxide Aggregate Root Canal Sealers Comparative Study

Zeid¹,

Saleh²

2014

J Trauma Treat

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Introduction: Bioceramic technology has been introduced recently in endodontics to benefit from the formation of hydroxyapatite during the setting reaction in the presence of tissue fluid and establish a chemical bond at the dentin interface. Objectives: To evaluate some of physiochemical properties of new different Bioceramic (iRoot-SP, EndoSequence, Smartpastebio) versus mineral trioxide aggregate (MTA-Fillapex) root canal sealers. Adseal and ActiV-GP sealers were used as control. Methods: Standard discs (10 for each) were prepared and immersed in 20 ml deionized water. After 1, 7, 14 and 28 days, solubility, pH changes and released elements were calculated and statistically analyzed with ANOVA test. Results: A significantly greater solubility and higher alkalinity were displayed by the tested sealers (in descending order) Smartpastebio, iRoot-SP, EndoSequence and MTA-Fillapex (P<0.05). Their solubility exceeded the acceptable limit (3%). Their maximum alkaline pH was exhibited after 7 days. Adseal and ActiV-GP exhibited initial neutral and acidic pH respectively. Finally both had neutral pH. EndoSequence exhibited the significantly greatest calcium release followed by iRoot-SP, Smartpastebio and MTA-Fillapex, whereas, ActiV-GP and Adseal exhibited the significantly lowest values (P<0.05). There was no silicon released from iRoot-SP, Smartpastebio and MTA-Fillapex. ActiV-GP exhibited the greatest silicon, aluminum and iron release. The greatest phosphorous, manganese and magnesium content was obtained by Adseal, MTA-Fillapex and EndoSequence respectively. Conclusion: Under the condition of this study, the prolonged alkalinity of calcium silicate sealers was synchronized with their ongoing solubility. The higher calcium ions released indicated strong alkalinity. The inequality in aluminum, iron, manganese and magnesium released by the tested sealers may expect the variance in their behaviors.

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Section: Discussionsupporting

confidence: 88%

Solubility, pH Changes and Releasing Elements of Different Bioceramic and Mineral Trioxide Aggregate Root Canal Sealers Comparative Study

Zeid¹,

Saleh²

2014

J Trauma Treat

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“…6 A previous study reported a very low inhibitory effect of Adseal against E. faecalis. 22 This supported our findings that showed high optical density (OD) of the unmodified sealer group (Table 2). However, a significant decrease in the OD was observed after sealer impregnation with GTE-CSNPs, indicating the considerable improvement in the antibacterial activity of Adseal.…”

Section: Discussionsupporting

confidence: 89%

Antimicrobial Activity, Physical Properties and Sealing Ability of Epoxy Resin-Based Sealer Impregnated with Green Tea Extract-Chitosan Microcapsules: An In-Vitro Study

Habib

Sadek

Hasanin

et al. 2021

Egyptian Dental Journal

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Aim: To assess the effect of prepared green tea-chitosan microcapsules on the antibacterial efficiency against Enterococcus faecalis, the physical properties, and the sealing ability of resinbased endodontic sealer impregnated with these microcapsules. Materials and Methods:Green tea extract (GTE) and chitosan solution were prepared, then GTE was dissolved into this solution followed by precipitation of chitosan nanoparticles (CSNPs) loaded with GTE. Microcapsulation of GTE-CSNPs was performed and the solution was prepared (3 % w/v concentration). Characterization of microcapsules was conducted using Fourier-Transform Infrared Spectroscopy (FTIR), Zeta potential, and transmission electron microscope (TEM). The release of GTE at different pH values was evaluated. GTE-CSNPs solution was added to an epoxy resin-based sealer and the antibacterial activity was evaluated via direct contact test. The physical properties of the modified sealer were evaluated according to American Dental Association (ADA) specifications. Sealing ability was evaluated stereomicroscopically. Data were statistically analyzed.Results: FTIR and Zeta potential ensured the attachment between GTE and Chitosan. The particle size of CSNPs and GTE-CSNPs was around 20 nm, while microcapsules were greater than 1000 nm. A significantly higher percentage of GTE was released at pH 5 than pH 7.4, (P < 0.001). After sealer modification, the antibacterial evaluation showed significant decrease in optical density (P < 0.001). Also, the setting time, film thickness and microleakage were significantly decreased, however, the solubility was significantly increased (P < 0.001) but still within the acceptable limit of ADA specifications.Conclusions: GTE-Chitosan microcapsule is a proper natural candidate for improvement of sealer's properties.

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“…Similar to the present findings, they showed that AdSeal was more biocompatible than the other two sealers; however, in contrast to the present results, they reported no cytotoxicity for AdSeal. Some other studies reported higher biocompatibility of AdSeal than AH Plus [37,38]. Although both of these sealers are epoxy resin sealers, higher biocompatibility of AdSeal compared with AH Plus may be due to the presence of calcium phosphate in its composition since calcium phosphate has high biocompatibility and induces bone formation [39].…”

Section: Discussionmentioning

confidence: 99%

In vitro comparison of the cytotoxicity of different endodontic sealers; AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal

Dastorani¹,

Aliee²,

Halabian³

et al. 2021

Preprint

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Background: This study aimed to assess the cytotoxicity of four commonly used endodontic sealers namely AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal against human gingival fibroblasts (HGFs). Methods: After culturing the HGFs, they were exposed to the respective sealers in set form and in five different weights, after sterilization. The cytotoxicity of the sealers was evaluated after 1, 3 and 7 days using the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed by repeated measures ANOVA. Results: After 24 h, all sealers showed low cytotoxicity. However, all sealers in 250 mg and 500 mg weights showed significantly higher cytotoxicity than the negative control group at 72 h, and 7 days (P<0.05) except for AdSeal in 80 mg weight (P>0.05). AH Plus was significantly more cytotoxic than other sealers at 3 and 7 days (P<0.05) while AdSeal had the closest results to the negative control group, and showed significantly higher biocompatibility than other sealers in 250 mg concentration. Conclusion: AdSeal showed the highest biocompatibility while AH Plus had the highest cytotoxicity among the tested sealers. Thus, its application may delay the healing of periapical lesions.

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Cytotoxicity and antibacterial property of new resin-based sealer

Cited by 10 publications

References 12 publications

Solubility, pH Changes and Releasing Elements of Different Bioceramic and Mineral Trioxide Aggregate Root Canal Sealers Comparative Study

Solubility, pH Changes and Releasing Elements of Different Bioceramic and Mineral Trioxide Aggregate Root Canal Sealers Comparative Study

Antimicrobial Activity, Physical Properties and Sealing Ability of Epoxy Resin-Based Sealer Impregnated with Green Tea Extract-Chitosan Microcapsules: An In-Vitro Study

In vitro comparison of the cytotoxicity of different endodontic sealers; AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal

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