Cytotoxicity and anti-microbial analysis of silver and graphene oxide bio nanoparticles

Nasim, Iffat; Kumar, Shobhit; Vishnupriya, V.; Jabin, Zohra

doi:10.6026/97320630016831

Cited by 21 publications

(16 citation statements)

References 18 publications

(17 reference statements)

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“…Although graphene and its derivatives can inhibit cariogenic bacteria, most of them are studied together with antimicrobial metals or non-metal or polymer nanoparticles, such as copper, silver, zinc, peptides, and polymer nanoparticles, to improve the antibacterial properties or facilitate the sustainable release of incorporated nanoparticles [ 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. They are also studied with existing dental materials, especially incorporated into restorative cements, either to improve antibacterial properties against Streptococcus mutans , reduce dental hard tissue demineralization, or facilitate remineralization [ 60 , 63 , 64 , 66 , 72 , 73 , 74 ].…”

Section: Resultsmentioning

confidence: 99%

“…Simultaneously, graphene oxide nanosheets were demonstrated effective in reducing Streptococcus mutans [ 60 , 61 ]. Subsequently, metal functionalized graphene materials, graphene-silver nanoparticles, were also found effective against Streptococcus mutans without any significant cytotoxicity [ 13 , 57 , 64 , 65 ]. Similarly, reduced graphene-silver nanoparticles and graphene-nanoplatelets doped silver nanoparticles showed an antibiofilm effect against Streptococcus mutans biofilm [ 58 ].…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review

et al. 2022

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Graphene is a single-layer two-dimensional carbon-based nanomaterial. It presents as a thin and strong material that has attracted many researchers’ attention. This study provides a concise review of the potential application of graphene materials in caries and periodontal disease management. Pristine or functionalized graphene and its derivatives exhibit favorable physicochemical, mechanical, and morphological properties applicable to biomedical applications. They can be activated and functionalized with metal and metal nanoparticles, polymers, and other small molecules to exhibit multi-differentiation activities, antimicrobial activities, and biocompatibility. They were investigated in preventive dentistry and regenerative dentistry. Graphene materials such as graphene oxide inhibit cariogenic microbes such as Streptococcus mutans. They also inhibit periodontal pathogens that are responsible for periodontitis and root canal infection. Graphene-fluorine promotes enamel and dentin mineralization. These materials were also broadly studied in regenerative dental research, such as dental hard and soft tissue regeneration, as well as periodontal tissue and bone regeneration. Graphene oxide-based materials, such as graphene oxide-fibroin, were reported as promising in tissue engineering for their biocompatibility, bioactivity, and ability to enhance cell proliferation properties in periodontal ligament stem cells. Laboratory research showed that graphene can be used exclusively or by incorporating it into existing dental materials. The success of laboratory studies can translate the application of graphene into clinical use.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review

et al. 2022

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“…1 g of the powdered leaves was dissolved in 100 ml of distilled water and mixed well and then the solution was boiled for 5 min at 60°C–80°C and filtered and stored in a refrigerator for later use. [ 11 ]…”

Section: Methodsmentioning

confidence: 99%

Green synthesis of calcium hydroxide-coated silver nanoparticles using Andrographis paniculata and Ocimum sanctum Linn. leaf extracts: An antimicrobial and cytotoxic activity

Nasim

Jabin²,

Kumar³

et al. 2022

J Conserv Dent

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Context: Silver is known for its antibacterial properties since ages. As nanoparticles have smaller size and greater surface area, silver has been utilized in the form of nanoparticles to enhance its antibacterial properties. Calcium hydroxide is a well-known intracanal medicament and serves as a gold standard for root canal disinfection. Using herbal extracts as reducing agents for nanoparticle synthesis appears to be an ecofriendly approach. Aim: The aim of this study was to synthesize calcium hydroxide-based silver nanoparticles using herbs as reducing agents and to test the cytotoxic levels and antimicrobial activity against oral microbes. Materials and Methods: The calcium hydroxide-based silver nanoparticles were synthesized using the leaves of Andrographis paniculata and Ocimum sanctum Linn. Various properties of the synthesized nanoparticles were also characterized by ultraviolet (UV) spectrophotometer analysis, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR) analysis. The cytotoxic effects of these nanoparticles were analyzed using brine shrimp and MTT assay. Antimicrobial activity was assessed by measuring the zone of inhibition. The statistical analysis was done using parametric independent t -test. P value was set at < 0.05. Results: The calcium hydroxide-based silver nanoparticles were successfully synthesized and were confirmed by UV spectrophotometer analysis, TEM, X-ray diffraction (XRD), and FTIR analysis and showed a minimal cytotoxic effect. They also showed a good antimicrobial activity and a remarkable antifungal activity. Conclusions: The green synthesis of CaOHAgNPs yielded an effective nanoparticle preparation that could be used against common oral pathogens as a potential therapeutic agent in the form of root canal irrigant or intracanal medicament in the field of dentistry.

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“…The consistency of being harmful to cells is known as cytotoxicity. The integrity of cell membranes is often impaired by cytotoxic compounds [15]. The main purpose of cancer chemotherapy is to kill cancer cells directly while avoiding toxicity of healthy cells.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Anti Inflammatory and Cytotoxic Effect of Copper Nanoparticles Synthesised Using Seed Extract of Mucuna pruriens

Anushya

Geetha

Kumar

2021

JPRI

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Introduction: Nanotechnology is a rapidly developing interdisciplinary area that has brought enormous changes in dentistry. Copper nanoparticle made from plant extract would be an environmental friendly, convenient and dependable way for providing therapeutic agents that are safe, free of side effects and useful for a wide range of diseases. Mucuna pruriens seed extract was selected for our study, due to its due to its anti- bacterial, anti-diabetic, anti- Parkinson, anti-cholesterol and anti- oxidant properties. Aim: To evaluate the anti-inflammatory properties of Mucuna pruriens, the green synthesis, characterization of CuNPs, and screening of their cytotoxic activity. Materials and methods: The collection and preparation of Mucuna pruriens extract was done and stored for further use. Synthesis of Cu nanoparticles was done with 30 milli molar of copper (II) sulfate. Centrifugation was done and characterisation of Copper nanoparticles using ultraviolet (UV)-visual spectrophotometer. Cytotoxic effect and anti-inflammatory activity of copper nanoparticles with Mucuna pruriens seed extract were assessed using Brine Shrimp Assay at 5µL, 10µL, 20µL, 40µL and 80 µL and Bovine Serum Albumin (BSA) at 5 µL, 10 µL, 20 µL, 30 µL, 50 µL. Results: The anti inflammatory activity of Copper Nanoparticles with Mucuna pruriens, increased with increase in concentrations. Percentage of inhibition was 17% at 10 µL concentration, 24% at 20 µL, 43% at 30 µL and 54% at 40 µL and highest at 50 µL (71%). Cytotoxicity of Copper Nanoparticles with Mucuna pruriens, at 5 µL concentration there was a death of 10% of nauplii, at 10 µL there was a death of 20% of nauplii, at 20 µL and 40 µL there was a death of 30% of nauplii and at 80 µL there was a death of 40% of nauplii. As the concentration increased, the cytotoxicity of the nanoparticles increased. Conclusion: Based on the results of the current study, it is concluded that Mucuna pruriens mediated Cu Nps can be used as a potential source of anti inflammatory agent and also as an anti cancer drug for the treatment of tumours and cancers.

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Cytotoxicity and anti-microbial analysis of silver and graphene oxide bio nanoparticles

Cited by 21 publications

References 18 publications

In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review

In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review

Green synthesis of calcium hydroxide-coated silver nanoparticles using Andrographis paniculata and Ocimum sanctum Linn. leaf extracts: An antimicrobial and cytotoxic activity

Evaluation of Anti Inflammatory and Cytotoxic Effect of Copper Nanoparticles Synthesised Using Seed Extract of Mucuna pruriens

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