Cytotoxic test of extract and fractions from Blumea balsamifera leaves using Brine Shrimp Lethality Test (BSLT)

Rahmi, Azimatur; Afriani, Tika; Aini, Aini

doi:10.20885/jif.vol18.iss1.art3

Cited by 4 publications

(5 citation statements)

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“…The percentage of mortality of Artemia salina larvae can be calculated using the equation (Sumihe, et al 2014). Using the linear regression equation is y = bx + a; y = 50% mortality in probit percentage, a = intercept or value obtained from the linear regression equation, x = log concentration, so that the LC50 value is obtained (Rahmi, et al, 2022). If LC50 < 30 ppm it is said to be very toxic, LC50 < 1000 ppm is said to be toxic, while LC50 > 1000 ppm then the test preparation is not toxic (Dona, et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

“…The BSLT method cytotoxic test used Artemia salina larvae that were 48 hours old because at this time the larvae already had a high level of sensitivity due to their complete organs (Rahmi, et al, 2022). Based on its morphology, Artemia salina larvae that are 48 hours old have started to have mouths and digestive tracts but their food reserves have started to run out so they start looking for food (Fadhli & Hasanah, 2019).…”

Section: Resultsmentioning

confidence: 99%

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Cytotoxic Test of Ethanol Extract of Bintaro Fruit and Peel (Cerbera odollam Gaertn.) against Artemia salina Leach Larvae

Handayani¹,

Syarif²,

Rahma³

2023

ijcsrr

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Bintaro (Cerbera odollam Gaertn). Plants belonging to the Apocynaceae family have properties such as antifungal, insecticide, antioxidant and antitumor. Bintaro fruit contains alkaloids, flavonoids, terpenoids, tannins and saponins. Bintaro plants are poisonous because they contain alkaloid group compounds which are toxic. This study aims to obtain data and information regarding the toxicity ratio of bintaro rind and bintaro fruit (Cerbera odollam Gaertn.) to Artemia salina Leach larvae by determining the value of Lethal Concentration 50 (LC50). This type of research was experimental with quantitative methods, using Artemia salina Leach larvae. Each extract was divided into 4 concentration groups. In the ethanol extract of bintaro fruit peel, namely at concentrations of 500, 1000, 1500 and 2500 ppm. Meanwhile, bintaro fruit extract at concentrations of 1000, 1500, 2000 and 2500 ppm, each of which was carried out 3 replications with 10 larvae. Data analysis using probit analysis showed that the ethanol extract of bintaro fruit peel (Cerbera odollam Gaertn.) had LC50 = 701.455 μg/mL which was categorized as toxic. Meanwhile, the ethanol extract of bintaro fruit (Cerbera odollam Gaertn.) has LC50 = 1199.5 μg/mL which is categorized as nontoxic.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Cytotoxic Test of Ethanol Extract of Bintaro Fruit and Peel (Cerbera odollam Gaertn.) against Artemia salina Leach Larvae

Handayani¹,

Syarif²,

Rahma³

2023

ijcsrr

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“…Golongan senyawa metabolit sekunder seperti alkaloid, flavonoid, tanin, dan terpenoid yang terkandung pada ekstrak etanol, fraksi air, fraksi etil astetat, dan fraksi n-heksan dapat bertindak sebagai stomach poisoning atau racun perut, sehingga apabila senyawa-senyawa tersebut masuk ke dalam tubuh larva udang maka alat pencernaanya akan terganggu, dapat menghambat reseptor perasa pada daerah mulut larva udang, dan tidak bisa mendapatkan stimulus rasa sehingga tidak mampu mengenali makanannya. Hal ini menyebabkan larva udang mengalami kematian (Rahmi et al, 2022).…”

Section: Grafik 2 Grafik Antara Log Konsentrasi Dan Nilai Probit Frak...unclassified

Uji Sitotoksik Ekstrak Etanol Kulit Batang Awar-awar dengan Metode BSLT

Alya Hermawanfutri,

Siti Hazar

2023

JRF

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Abstract. Cancer is a disease that is a major health problem in the world. Pulai leaves (Alstonia scholaris (L.) R. Br.) are part of a plant that is thought to have anticancer potential because it’s contain secondary metabolites such as alkaloid. Alkaloid compounds are known to have activity that can inhibit growth and kill cancer cells. This study aims to determine the cytotoxic activity and LC50 value of the ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction of pulai leaves. The cytotoxic test was carried out using the Brine Shrimp Lethality Test (BSLT) method on Artemia franciscana Kellogg shrimp larvae and probit analysis to determine the LC50 value. The test concentrations used were 10 ppm, 100 ppm, 250 ppm, 500 ppm, 750 ppm, and 1000 ppm. Testing the cytotoxic activity of the ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction of pulai leaves produced LC50 values of 207,2526 ppm, 282,1630 ppm, 187,5858 ppm, and 484,2839 ppm, respectively. This implied that the ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction of pulai leaves have a cytotoxic effect and have anticancer potential as indicated by an LC50 value of less than 1000 ppm. Abstrak. Kanker merupakan salah satu penyakit yang menjadi masalah kesehatan utama di dunia. Daun pulai (Alstonia scholaris (L.) R. Br.) merupakan bagian tanaman yang diduga memiliki potensi sebagai antikanker karena mengandung golongan senyawa metabolit sekunder seperti alkaloid. Golongan senyawa alkaloid diketahui memiliki aktivitas yang dapat menghambat pertumbuhan dan membunuh sel kanker. Penelitian ini bertujuan untuk mengetahui aktivitas sitotoksik dan nilai LC50 dari ekstrak etanol, fraksi air, fraksi etil asetat, dan fraksi n-heksan daun pulai. Uji sitotoksik dilakukan dengan metode Brine Shrimp Lethality Test (BSLT) terhadap larva udang Artemia franciscana Kellogg dan analisis probit untuk mengetahui nilai LC50. Konsentrasi uji yang digunakan adalah 10 ppm, 100 ppm, 250 ppm, 500 ppm, 750 ppm, dan 1000 ppm. Pengujian aktivitas sitotoksik ekstrak etanol, fraksi air, fraksi etil asetat, dan fraksi n-heksan daun pulai menghasilkan nilai LC50 berturut-turut sebesar 207,2526 ppm, 282,1630 ppm, 187,5858 ppm, dan 484,2839 ppm. Hal ini menunjukkan bahwa ekstrak etanol, fraksi air, fraksi etil asetat, dan fraksi n-heksan daun pulai memiliki efek sitotoksik dan berpotensi sebagai antikanker yang ditandai dengan nilai LC50 kurang dari 1000 ppm.

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“…Inhibitory concentration (IC50) Meyer et al, (1982) dimana suatu sampel atau bahan uji (ekstrak) dikatakan toksik apabila nilai LC50 ≤ 1000 ppm, sedangkan dikatakan tidak toksik jika nilai LC50 ≥ 1000 ppm. Menurut Rahmi et al, (2022) mekanisme sitotoksik bekerja dengan cara menghambat reseptor indra perasa sekitar mulut Artemia salina (larva udang), sehingga larva tersebut tidak menerima rangsangan dan tidak mampu mengenali asupan makanan sehingga mengakibatkan kematian pada larva udang. Efek sitotoksik yang mengakibatkan kematian larva udang yang dihasilkan suatu bahan uji pada kadar tertentu dikarenakan adanya kelompok senyawa metabolit sekunder.…”

Section: Tabel 4 Klasifikasi Aktivitas Antioksidan Terhadapunclassified

Aktivitas antioksidan dan uji sitotoksik infusa daun jarak pagar (Jatropha curcas)

Rahman,

Putri,

Toepak

et al. 2023

SJP

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Antioxidants are crucial in preventing cell damage caused by oxidation from oxidants. The use of antioxidants from natural products such as Jatropha curcas leaves can counteract free radicals due to the presence of secondary metabolites. The main aims of the study were to determine the antioxidant and cytotoxic activity of the infusion of J. curcas leaves. The sample was extracted using the infusion method. A qualitative phytochemical screening was carried out using the test tube method, antioxidant assay using the DPPH method and cytotoxic using the BSLT method. The results showed that the infuse of J. curcas leaves contained secondary metabolites such as polyphenols and tannins, alkaloids, flavonoids, and saponins. In the antioxidant activity test, infuse of J. curcas leaves and vitamin C (control) the IC50 was 58.90±2.34 ppm and 8.78±0.21 ppm respectively. The infused cytotoxic test contained an active toxic compound in shrimp larvae with an LC50 of 200.28 ppm (LC50 ≤ 1000 ppm). Based on the results of the study, infuse of J. curcas leaves showed potential as an antioxidant and anticancer agent with a strong classification.

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Cytotoxic test of extract and fractions from Blumea balsamifera leaves using Brine Shrimp Lethality Test (BSLT)

Cited by 4 publications

References 0 publications

Cytotoxic Test of Ethanol Extract of Bintaro Fruit and Peel (Cerbera odollam Gaertn.) against Artemia salina Leach Larvae

Cytotoxic Test of Ethanol Extract of Bintaro Fruit and Peel (Cerbera odollam Gaertn.) against Artemia salina Leach Larvae

Uji Sitotoksik Ekstrak Etanol Kulit Batang Awar-awar dengan Metode BSLT

Aktivitas antioksidan dan uji sitotoksik infusa daun jarak pagar (Jatropha curcas)

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