Cytotoxic effects of the root extracts ofEurycoma longifolia Jack

Abstract

The methanol, n-butanol, chloroform and water extracts obtained from the root of Eurycoma longifolia Jack were assayed using methylene blue assay to evaluate its cytotoxic effect against KB, DU-145, RD, MCF-7, CaOV-3, MDBK cell lines. The results showed that all the root extracts except the water extract of E. longifolia produced significant cytotoxic effect on these cell lines. However, no significant cytotoxic effect was detected on MDBK (kidney) normal cell line. 9-methoxycanthin-6-one, an alkaloid, was detected in each extract with different intensities by reversed-phase high performance liquid chromatography.

Habitat Preferences, Distribution Pattern, and Root Weight Estimation of Pasak Bumi (Eurycoma longifolia Jack.)

“…Certain studies report that pharmacologically, E. longifolia Jack. contains anticancer compound (Nurhanan et al 2005;Tee et al 2007), anti-bacteria (Farouk & Benafri 2007), osteoporosis therapy for men (Effendi et al 2012), cyto-toxic (Kuo et al 2003;Kuo et al 2004), aphrodisiac (Ang et al 2003;, antileukemia, anti-malaria, and dysentery (Chan et al 2005). Due to its various benefits, there has been a substantial increase in its demands for medicinal ingredients.…”

In vitro anti-microbial activity of the Cuban medicinal plants Simarouba glauca DC, Melaleuca leucadendron L and Artemisia absinthium L

“…A significant cytotoxicity observed in the present study was also reflected in a toxic action after intraperitoneal injection in mice (Rodríguez et al 2006). Several constituents have been isolated from Simaroubaceae, mainly alkaloids with high cytotoxicity and quassinoids with antimalarial and cytotoxic characteristics (Nurhanan et al 2005). The antiplasmodial and cytotoxic properties of quassinoids are both linked to protein synthesis inhibition (Fukamiya et al 2005), and it is likely that parasite and host cell ribosomes are too similar to allow for the development of selective inhibitors (Wright 2005).…”

Efek Ko-Kemoterapi Fraksi Etil Asetat Akar Pasak Bumi Dan Doxorubicin Terhadap Proliferasi Dan Ekpresi Bax Jaringan Payudara Tikus Sd

“…Senyawa antikanker dan kokemoterapi sebagai alternatif pengobatan dapat diperoleh dari akar pasak bumi (Eurycoma longifolia Jack). Akar pasak bumi (Eurycoma longifolia Jack) mengandung kuasinoid (Bedir et.al.,2003), alkaloid 9-metoksisantin-6-on (Nurhanan, et al, 2005), alkaloid canthinone (Chan et al, 2002), dan flavonoid (Nurani, 2011). Tsui Tee (2005) menyatakan bahwa ekstrak dan fraksi E. longifolia memiliki aktivitas antiproliferatif pada MCF-7 dengan menginduksi apoptosis melalui modulasi protein famili Bcl-2.…”