Efek Ko-Kemoterapi Fraksi Etil Asetat Akar Pasak Bumi Dan Doxorubicin Terhadap Proliferasi Dan Ekpresi Bax Jaringan Payudara Tikus Sd

Zakinah, Tria; Nurani, Laela Hayu; Widyarini, Sitarina

doi:10.24071/jpsc.141559

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Mekanisme apoptosis yang diinduksi flavonoid pada sel kanker dengan menghambat aktivitas DNA topoisomerase I/II, mengatur jalur pensinyalan, mengurangi ekspresi gen Bcl-2 dan Bcl-XL, meningkatkan ekspresi gen Bax dan Bak, dan mengaktifkan endonuclease (Pebriana, 2008). Selain itu, kandungan flavonoid dapat menginduksi kerusakan DNA irreversible dan apoptosis sel T47D dapat terjadi melalui jalur p53 yang ditandai dengan interaksi flavonoid dengan DNA topoisomerase (Zakinah et al, 2017). Cell cycle arrest atau cell cycle delay merupakan salah satu model pengamatan kinetika proliferasi sel untuk mengetahui terjadinya penghambatan proliferasi sel.…”

Section: Pendahuluanunclassified

STUDI IN SILICO SENYAWA KUARSETIN DAUN KENCANA UNGU (Ruellia tuberosa L.) SEBAGAI AGEN ANTIKANKER PAYUDARA

Shofi

2022

Jurnal Sintesis

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Penyakit kanker merupakan salah satu penyakit degeneratf yang sangat membahayakan, salah satunya kanker payudara yang banyak menyerang wanita. Pengobatan selama ini masih menggunakan kemoterapi, operasi dan radioterapi. Salah satu alternatif pengobatan kanker yaitu menggunakan tanaman herbal yaitu daun kencana ungu (Ruellia tuberosa L.). pada daun tanaman ini banyak terkandung kuarsetin yang dapat menghambat pertumbuhan sel kanker. Penelitian ini bertujuan untuk menganalisis kandungan kuarsetin pada daun kencana ungu sebagai agen anti kanker payudara melalui penghambatan Enzim Sirtuin1. Metode penelitian ini adalah struktur 3D enzim Sirtulin1 (pdb id : 4I5I), senyawa kuarsetin (ID : 5280343) dipreparasi dengan program PyRx. Molekular doking dianalisis dengan interaksi kuarsetin dengan enzim Sirtulin1 menggunakan software Autodock 4.2. dan divisualisasikan dengan discovery studio versi 4.1. Analisis data menggunakan secara deskriptif. Hasil penelitian kuarsetin dengan Sirtulin1 memiliki 16 residu asam amino (Glu420, Leu418, Lys377, Glu410, Glu 416, Lys375, Ser370, Cys371, Leu372, Lys408, Pro409, Asn417, Ile411, Val412, Pro419, Gln412) dan ikatan hidrogen. Kuarsetin daun kencana ungu memiliki potensi aktivitas sebagai anti kanker panyudara karena memiliki afinitas dengan Sirtulin1 yang dapat mencegah terjadinya pengaktifan proses gen dalam pembentukan antioksidan.

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Section: Pendahuluanunclassified

STUDI IN SILICO SENYAWA KUARSETIN DAUN KENCANA UNGU (Ruellia tuberosa L.) SEBAGAI AGEN ANTIKANKER PAYUDARA

Shofi

2022

Jurnal Sintesis

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“…As mentioned in equation ( 4), the calculation of pro tein expression based on research conducted by Zakinah et al (2017) was carried out on a minimum of 100 cells from each point of view. Furthermore, the observation of the samples was carried out from three different points of view for each sample and documented with a camera.…”

Section: Percentage Of Cells With Protein Expression =mentioning

confidence: 99%

Cytotoxic effects of parijoto (Medinilla speciosa Reinw. Ex. Bl.) methanol extract combined with cisplatin on WiDr colon cancer cells through apoptosis induction

Artanti

Prihapsara

Susanto

2022

Indones. J. Biotechnol.

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Parijoto (Medinilla speciosa Reinw. Ex. Bl.) is a medicinal plant with cytotoxic effects on cancer cells in vitro. As only a limited number of studies have reported the effect of parijoto on colon cancer cells, this study initially aimed to measure the total flavonoid levels and potential cytotoxic effects of parijoto methanol extract (PME) through cell viability assays and expression of the apoptotic protein on WiDr colon cancer cells as a model. PME cytotoxic activity was determined by conducting a cytotoxicity test on WiDr colon cancer cells using the MTT assay. The synergistic cytotoxic effects of the PME and cisplatin were tested to obtain the combination index (CI) value. Apoptosis was analyzed by flow cytometry, and the apoptotic protein expression was observed by immunocytochemical tests. Furthermore, quercetin as a major flavonoid in PME was measured using a UV–Vis spectrophotometer. The results showed that PME had a moderate cytotoxic activity with an IC50 of 198.64±1.6 µg/mL, whereas the IC50 of cisplatin was 2.34±0.7 µg/mL. The PME with cisplatin combination test showed a strong synergistic effect with a CI value of <1 (0.1‐0.4). The combination showed increased apoptosis properties compared to PME treatment alone. In addition, immunocytochemistry showed that PME alone or in combination with cisplatin increased the pro‐apoptosis proteins (p53 and caspase‐9) and suppressed Bcl‐2 expression. Moreover, the cell viability value increased as the PME concentration decreased. The administration of PME led to changes in cell morphology, lower cell density, and a decreasing number of living cells. Therefore, the combination of PME and cisplatin had a strong synergistic effect in inducing apoptosis.

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Efek Ko-Kemoterapi Fraksi Etil Asetat Akar Pasak Bumi Dan Doxorubicin Terhadap Proliferasi Dan Ekpresi Bax Jaringan Payudara Tikus Sd

Cited by 2 publications

References 17 publications

STUDI IN SILICO SENYAWA KUARSETIN DAUN KENCANA UNGU (Ruellia tuberosa L.) SEBAGAI AGEN ANTIKANKER PAYUDARA

STUDI IN SILICO SENYAWA KUARSETIN DAUN KENCANA UNGU (Ruellia tuberosa L.) SEBAGAI AGEN ANTIKANKER PAYUDARA

Cytotoxic effects of parijoto (Medinilla speciosa Reinw. Ex. Bl.) methanol extract combined with cisplatin on WiDr colon cancer cells through apoptosis induction

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